Home G(5')ppp(5')G RNA Cap Structure Analog

G(5')ppp(5')G RNA Cap Structure Analog

  • Co-transcriptional capping with T7, SP6 and T3 RNA polymerases
  • Synthesis of unmethylated G-capped RNA

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Catalog # Concentration Size
S1407S Not Applicable 1 µmol
S1407L Not Applicable 5 µmol
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  • The 5´ terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

    A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (7). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5´)ppp(5´)G (6).

    Sequence

    G(5')ppp(5')G
    This product is related to the following categories:
    RNA Capping

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Supplied As

      Dried Sodium Salt

      Suggested Working Concentration

      10mM

      Recommended Resuspension Volume

      100 μl

    • Product Notes

      1. Addition of 100 µl of water gives approximately a 10 mM solution.

    • References

      1. Shatkin, A.J. (1978). Cell. 9, 645-653.
      2. Fillipowicz, W. (1978). FEBS Lett. . 96, 1-11.
      3. Banerjee, A.K. (1980). Microbiol. Rev.. 1980, 175-205.
      4. Miura, K. (1981). Adv. Biophys. . 14, 205-238.
      5. Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
      6. Konarska, M.M. et al. (1984). Cell. 38, 731-736.
      7. Contreas, R. et al. (1982). Nucl. Acids Res. 10, 6353-6363.
      8. Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.

  • Protocols, Manuals & Usage

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  • Citations & Technical Literature

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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