Home m7G(5')ppp(5')A RNA Cap Structure Analog

m7G(5')ppp(5')A RNA Cap Structure Analog

Co-transcriptional capping with T7 RNA polymerase from the phi2.5 promoter that contains an A at the transcription initiation site.

  • Synthesis of m7G capped RNA for in vitro splicing assays.
  • Synthesis of m7G capped RNA for transfection or microinjection.

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Catalog # Concentration Size
S1405S Not Applicable 1 µmol
S1405L Not Applicable 5 µmol
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  • Description: The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

    A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (7). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5´)ppp(5´)G (6).

    Sequence

    7mG(5')ppp(5')A
    This product is related to the following categories:
    RNA Capping

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Supplied As

      Dried Sodium Salt

      Suggested Working Concentration

      10mM

      Recommended Resuspension Volume

      100 μl

    • Product Notes

      1. Addition of 100 µl of water gives approximately a 10 mM solution.

    • References

      1. Shatkin, A.J. (1978). Cell. 9, 645-653.
      2. Fillipowicz, W. (1978). FEBS Lett.. 96, 1-11.
      3. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
      4. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
      5. Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
      6. Konarska, M.M. et al. (1984). Cell. 38, 731-736.
      7. Contreas, R. et al. (1982). Nucl. Acids Res.. 10, 6353-6363.
      8. Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.

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