NEBNext® Ultra™ II RNA Library Prep (Directional & Non-directional)

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Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.

Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the "dUTP method") and non-directional library prep and are compatible with poly(A) mRNA enrichment or rRNA depletion. The kits are available with the option of SPRISelect® beads for size-selection and clean-up steps.

Why consider the NEBNext Ultra II Directional RNA Library Prep Kit?

  • Get more of what you need, with the highest library yields
  • Generate high-quality libraries even with limited amounts of RNA:
    • 10 ng – 1 µg Total RNA  (poly(A) mRNA workflow)
    • 5 ng – 1 µg Total RNA (rRNA depletion workflow)
  • Minimize bias, with fewer PCR cycles required
  • Increase the complexity and transcript coverage of your libraries
  • Maximize flexibility by ordering reagents for your specific workflow needs, including directional and non-directional kits, rRNA depletion and poly(A) mRNA isolation reagents, and adaptors and primers (12- and 96-index) sold separately
  • Enjoy the reliability of the gold standard SPRIselect size selection and clean-up beads, supplied in just the amounts you need
  • Save time with streamlined workflows, reduced hands-on time, and automation compatibility
  • Rely on robust performance, even with low quality RNA, including FFPE

Are you looking for non-directional library prep?

View performance data for our NEBNext Ultra II RNA Library Prep Kit: 

The Ultra II RNA kit has allowed us to reduce the input for directional polyA+ RNAseq libraries by a factor of 10 or more. We can now make a library with only 10 ng high quality total RNA and get the same gene expression profile as for 1 µg input. We've even pushed the input as low as 1 ng for very high quality total RNA. The new Ultra II RNA kit makes RNAseq achievable for low yield samples, we actually need more RNA for quality control than for library prep! Furthermore, the library prep protocol is streamlined compared to the previous Ultra RNA kits, including a reduction in AMpure bead cleanups and PCR cycles, resulting in better libraries for less time and resources.

– Jen Grenier, Ph.D.
   Director of RNA Sequencing Core (RSC), Center for
   Reproductive Genomics, Department of Biomedical
   Sciences, College of Veterinary Medicine, Cornell University

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

ILLUMINA®, TRUSEQ® and NEXTSEQ® are registered trademarks of Illumina, Inc.
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.
RIBO-ZERO™ is a trademark of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems

Available kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.

NEBNext_portal_product_icon 

NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
Includes optimized mixes for non-directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

NEBNext_portal_product_icon 

NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads
Includes optimized mixes for non-directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.

Request a sample

NEBNext® Ultra™ II Directional RNA Workflow

Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.

Tips for Optimizing RNA Inputs in NGS Library Construction

Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.

NEBNext_portal_Selector_icon  NEBNext Selector
Find the right kits for your DNA or RNA sample preparation with our interactive online tool