Are there any special concerns with electroporation after ligation?
Yes, actually, so, certain additives in ligation buffers can lead to arcing and inhibition during electroporation transformation. Of particular concern is polyethylene glycol, aka PEG, which is found in ligation buffers, for example, in the Quick Ligation Kit ligation buffer. For this reason, a lot of people will cleanup buffers before transformation by electroporation.
However, as an alternative, you could try NEB’s ElectroLigase, which is T4 DNA ligase along with an optimized buffer that allows for efficient ligation of even difficult to ligate ends, but is also directly compatible with electroporation without any clean up steps.
And remember if you have any addition questions you can always contact us via email at info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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