N-Glycopeptide Binding Protein

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0872S     -20       N-Glycopeptide Binding Protein P0872SVIAL -20 1 x 1 ml 1 mg/ml

Features

• Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
• Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
• Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
• Versatile workflow
  
  

Companion Products

• Trypsin-ultra™, Mass Spectrometry Grade
• Endoproteinase LysC
• SNAP-Capture Magnetic Beads
• 6-Tube Magnetic Separation Rack

1. Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
2. Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
3. Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.

1. N-Glycopeptide Binding Protein Typical Reaction Protocol (NEB #P8072)

1. How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?
2. What is the specificity of N-Glycopeptide Binding Protein?
3. How strong does the N-Glycopeptide Binding Protein bind to a glycopeptide?
4. Can N-Glycopeptide Binding Protein bind and enrich free glycans?
5. Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
6. What influences the binding affinity of the N-Glycopeptide Binding Protein?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0872S_v1_10236187

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• N-Glycopeptide Binding Protein

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