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Phosphatases

NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.

Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Quick Dephosphorylation Kit (NEB #M0508), Shrimp Alkaline Phosphatase (NEB #M0371), Calf Intestinal Alkaline Phosphatase (NEB #M0290), and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)). 

NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right DNA phosphatase, choose "End Modification" on the tool to start. 

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Protocols for Phosphatases
Application Notes for Phosphatases
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  • DephosphorylationVideo_thumb

    The Mechanism of Dephosphorylation

    Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases.

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