Home FAQs How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?

FAQ: How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?

N-glycopeptides typically constitute only a small fraction of the total proteolyzed peptide pool of a complex proteome sample; therefore, N-glycopeptides are often overwhelmed by more abundant non-glycopeptides in an LC-MS experiment. Additionally, the ions with the highest ionization efficiency, which are typically non-glycopeptides, are selected for MS/MS fragmentation to produce glycan compositional information and enable glycopeptide discovery. This can lead to severe underrepresentation of glycopeptides in an LC-MS run. Enrichment by N-Glycopeptide Binding Protein significantly reduces the overall number of non-glycopeptide species in a sample. More MS/MS cycle time will be dedicated to the enriched N-glycopeptides, achieving drastically improved N-glycopeptide spectra match and glycoprotein identification and discovery.