No. The volume of NEBNext Primers for Epigenetics has been optimized for use with the NEBNext Enzymatic 5hmC-seq Kit (NEB #E3350). NEBNext Primers for Epigenetics products do not include an adaptor, and the required adaptor is supplied in the NEBNext Enzymatic 5hmC-seq Kit.
The i5 and i7 unique dual indices associated with individual wells for the NEBNext Primers for Epigenetics (Unique Dual Index Set 3) (NEB #E3404S), are the same as those within the 96-well plate format of NEBNext Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 3) (NEB #E6444). The indices in NEBNext Primers for Epigenetics (Unique Dual Index Set 2B) (NEB #E3392S) are a subset of NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2) (NEB #E6442).
