FAQ: Can I use dUTP and UDG for carryover contamination prevention with the WarmStart LAMP Kit (DNA & RNA)?
Yes, the WarmStart LAMP Master Mix is compatible with dUTP/UDG carryover prevention. No dUTP is contained in the mix, but 700 μM dUTP can be spike into the mix without significant effects on LAMP performance. Note that it is important to use Antarctic Thermolabile UDG (NEB #M0372), as the isothermal temperature (65 °C) of the LAMP reaction is insufficient to inactivate E. coli UDG and use of the E. coli form can result in inhibition of LAMP. Simply add 0.02 U/μL Antarctic Thermolabile UDG and set up reactions at room temperature to destroy contaminating LAMP products.
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