LOCUS pRS416 4891 bp DNA circular 05-DEC-2007 DEFINITION Cloning vector pRS416, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pRS416 ORGANISM Cloning vector pRS416 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4891) AUTHORS Sikorski,R.S. and Hieter,P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae JOURNAL Genetics 122 (1), 19-27 (1989) PUBMED 2659436 REFERENCE 2 (bases 1 to 4891) AUTHORS Christianson,T.W., Sikorski,R.S., Dante,M., Shero,J.H. and Hieter,P. TITLE Multifunctional yeast high-copy-number shuttle vectors JOURNAL Gene 110 (1), 119-122 (1992) PUBMED 1544568 REFERENCE 3 (bases 1 to 4891) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (05-DEC-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession U03450. FEATURES Location/Qualifiers source 1..4891 /organism="Cloning vector pRS416" /mol_type="other DNA" gene 410..1213 /gene="URA3" CDS 410..1213 /gene="URA3" /codon_start=1 /product="orotidine-5'-phosphate (OMP) decarboxylase" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" rep_origin complement(1344..1799) /note="f1 origin of replication (+ -)" gene complement(1582..2160) /gene="lacZalpha" CDS complement(1582..2160) /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD" misc_feature 1997..2104 /gene="lacZalpha" /note="multiple cloning site (KpnI-SacI)" rep_origin complement(2558..3145) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point); rop gene has been disrupted" gene complement(3317..4177) /gene="bla" CDS complement(3317..4177) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(4109..4177) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" misc_structure 4318..4436 /note="CEN6 (yeast chromosome VI centromere sequence)" rep_origin 4449..4822 /note="ARS209; histone H4 autonomously replicating sequence (ARS)" BASE COUNT 1359 a 1077 c 1166 g 1289 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc 241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag 301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt 361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc 421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat 481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt 541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat 601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta 661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca 721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt 781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc 841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata 901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc 961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt 1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt 1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc 1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg 1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag 1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg 1321 taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt 1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 1801 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 1861 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 1921 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc 1981 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga 2041 tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga 2101 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat 2161 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa 2221 gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc 2281 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 2341 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 2401 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 2461 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 3601 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 3901 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat 4321 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat 4381 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac 4441 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta 4501 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta 4561 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa 4621 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct 4681 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa 4741 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg 4801 tgtaagttac aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat 4861 aaaaataggc gtatcacgag gccctttcgt c //