The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.
Tags DNA Modifying Enzymes and Cloning Technologies, Cloning & Synthetic Biology, Usage Guideline
Related Products Quick Blunting™ and Quick Ligation™ Kits, Quick Blunting™ Kit, T4 Polynucleotide Kinase, T4 DNA Polymerase, DNA Polymerase I, Large (Klenow) Fragment, T4 Polynucleotide Kinase (3' phosphatase minus), Mung Bean Nuclease