Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for re-assembly. View a list of TypeIIS enzymes.
Tags Restriction Endonucleases, DNA Modifying Enzymes and Cloning Technologies, DNA Assembly, Cloning and Mutagenesis Kits, Golden Gate Assembly
Related Products NEB® Golden Gate Assembly Mix, BsaI, BbsI, BtgZI, BspQI, BsaI-HF®, BbsI-HF®, BsaI-HF®v2