This video discusses Domestication, or the removal of Type IIS cut sites naturally occurring in vector or insert sequences, as it relates to Golden Gate Assembly.
Tags DNA Modifying Enzymes and Cloning Technologies, Golden Gate Assembly
Related Products NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2), T7 DNA Ligase, T4 DNA Ligase, NEBridge® Ligase Master Mix, Hi-T4™ DNA Ligase, BsaI, BbsI, SapI, BtgZI, BspQI, Esp3I, BsmBI-v2, PaqCI®, BbsI-HF®, BsaI-HF®v2