See Dr. Eva Nogales present her work on the "Structure and Dynamics of Macromolecular Complexes using Cryo-EM."
Video Library
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NEB TV Ep. 23 – Colorimetric LAMP in point-of-care diagnostics
Learn the basics of Colorimetric LAMP, and how groups like NEB and the World Mosquito Program are using this isothermal amplification method in field tests and point-of-care diagnostics.
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What is Pulsed Field Gel Electrophoresis?
Learn the basics of how large DNA molecules are separated with Pulsed Field Gel Electrophoresis (PFGE).
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Tips for successful RNA Cleanup using the Monarch® RNA Cleanup Kits
Optimize the results of your RNA cleanups with these quick and simple tips.
NGS Sample Prep & Target Enrichment
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Quick Tips - Preventing sorting carryover for the NEBNext Single Cell/Low Input RNA Kit
Learn how to prevent media carryover in cell sorting if you are unable to sort directly into the NEBNext Cell Lysis Buffer with murine RNase inhibitor. more
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Quick Tips - Bead Reconstitution Buffer for NEBNext Single Cell/Low Input RNA Library Prep
Learn why it is important that the Bead Reconstitution Buffer be at room temperature for use. more
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Quick Tips - Preparing the NEBNext Ultra II FS DNA Reaction Buffer and Enzyme Mix
Here are some important things to keep in mind when preparing the NEBNext® Ultra II FS DNA reaction buffer and enzyme mix for use. more
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Quick Tips - Vortexing the NEBNext Single Cell/Low Input RNA Library Prep Kit RT Buffer
Learn why vortexing the NEBNext Single Cell RT Buffer is so important. more
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Quick Tips - Preparing NEBNext Ultra II FS DNA Reaction Buffer and Enzyme Mix Master Mix
Learn how to make a master mix of the NEBNext Ultra II FS DNA reaction buffer and enzyme mix. more
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Quick Tips - Adding the lysis buffer for the PCR in the NEBNext RNA workflow
During the NEBNext Single Cell/Low Input RNA library prep workflow, adding lysis buffer to the cDNA PCR reaction increases efficiency. more
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Quick Tips – Freezing cells after sorting in the NEBNext Single Cell/Low Input RNA workflow
Learn how to best preserve RNA in your samples when freezing cells in the NEBNext Single Cell/Low Input RNA library prep workflow. more
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NEBNext® Magnetic Separation Rack
The NEBNext® Magnetic Separation Rack enables efficient bead separation, quickly pulling beads to the side, and allowing elution in small volumes. more
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NEBNext® Enzymatic Methyl-seq Workflow
A workflow animation for an enzyme-based method for detection of 5mC and 5hmC, an alternative to bisulfite sequencing. more
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Library prep for NGS
Get a quick overview of NGS library preparation from an NEB scientist. more
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NEBNext Direct® Target Enrichment
Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing. more
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NEBNext Direct® Workflow
View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow. more
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Size Selection and Cleanup with NEBNext Ultra II and SPRI beads
This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA more
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NEBNext Ultra II DNA Library Prep Protocol
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points. more
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Behind the Paper: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues
Can microbiome-enrichment techniques be used to partition plant DNA? Erbay describes the findings of his recent paper. more
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Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent® Sequencing
Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab. more
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Biomek Automated NEBNext Ultra Directional RNA Library Prep Kit for Illumina
Learn how Biomek automation of NEBNext RNA library preparation enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Zach Smith from Beckman Coulter describes strand-specific RNA library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and discusses Biomek automation of this method. more
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NEBNext Ultra II Directional RNA Workflow
Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit. more
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How Library Preparation Affects Sequencing Accuracy
In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary. more
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Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification
NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants. more
