Customers frequently ask “Why is there a white precipitate in my T4 DNA ligase buffer?”
If you see a white precipitate in your T4 DNA ligase buffer it is most likely DTT falling out of solution. It is important not to heat the buffer to resuspend the precipitate, as this will degrade the ATP, which is necessary for ligation. DTT is found in excess in the buffer and the small amount lost in the precipitation will not affect your ligation efficiency.
Remember, if you have any questions you can contact us at [email protected]
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.