For fragments that are eight kb or larger, we recommend that you add 1.5 volumes of water after the agarose is dissolved. This mitigates tighter binding of the larger DNA pieces to the matrix, allowing easier elution off the column.
For plasmids that are larger than 10 kb, heating the elution buffer to 50 degrees Celsius prior to adding it to the column can improve yields.
Remember, you can always get additional technical support by contacting firstname.lastname@example.org.
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