This overview will walk you through how the Polymerase Chain Reaction (PCR) works.
The polymerase chain reaction, or PCR, is a method for amplifying a fragment of DNA. To begin, the DNA template of interest is mixed with a DNA polymerase, oligonucleotide primers, dNTPs, and an appropriate reaction buffer. This reaction is placed into a thermocycler that is pre-programmed to modulate the temperature during PCR.
The first step of amplification reaction is a heating step known as melting, or denaturation, during which the hydrogen bonds that hold the strands together are broken. This effectively separates the two strands. Next, the temperature is decreased during the annealing step. This allows the primers to anneal with the complimentary sequence on the template DNA strands. During the third step of PCR, the temperature is raised slightly and the DNA polymerase extends the primer template duplex by adding the complimentary nucleotides one by one to the nascent DNA strand.
Once the first strand is copied, the process is repeated by cycling back to the first reaction temperature for the next round of denaturation, annealing, and extension. This three step temperature cycle is repeated approximately 30 times, which results in exponential application of the sequence of interest. This method of DNA amplification can create up to two billion copies of the original DNA only after 30 cycles.
Visit ConfidentPCR.com for help with selecting the right DNA polymerase for your experiment.
Choose your country
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.