Abundant transcripts in your sample? Where they come from and how to focus on sequences that matter to you. Part I
The large dynamic range of transcript expression presents a challenge in whole-transcriptome sequencing. Highly expressed transcripts, their sources, and how they can hinder detection of informative transcripts will be discussed. We have optimized a method to enrich for transcripts of interest by eliminating specific RNAs before sequencing. This method is based on the hybridization of probes to unwanted RNAs followed by enzymatic degradation of the targeted RNAs and probes. In this webinar we present our latest solutions, including a customization option, to enrich for RNAs of interest across diverse species.
In part 1, Deyra N. Rodriguez and Bradley W. Langhorst will present advances in our method to eliminate cytoplasmic and mitochondrial ribosomal RNA (rRNA) from human, mouse, and rat total RNA samples. This method has been successfully applied to a range of total RNA samples including from formalin-fixed-paraffin-embedded (FFPE). Additionally, they will present a solution to remove abundant adult, fetal and embryonic globin transcripts from blood RNA samples in human, mouse and rat.
Please join us after the webinar for an opportunity to engage in discussion and ask questions of the NEB experts.