• My NEB
  • Print
  • PDF
  • Reduced Star Activities of HF® Enzymes

    The following table indicates the number of units of HF®-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.

    EnzymeBufferMax Units Without Star ActivityHF Factor
    AgeI (1) (2)NEBuffer 1.132
    AgeI-HF® (2)CutSmart® Buffer≥250≥8
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    BamHI (1)NEBuffer 3.132
    BamHI-HF®CutSmart® Buffer≥4,000≥125
    BmtI (1)NEBuffer 3.132
    BmtI-HF®CutSmart® Buffer100000062500
    BsaI (1)CutSmart® Buffer32
    BsaI-HF®CutSmart® Buffer≥8,000≥250
    BsrGI-HF®CutSmart® Buffer
    BstEII  (1) (2)NEBuffer 3.116
    BstEII-HF®CutSmart® Buffer>2000>125
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    DraIII (1)NEBuffer 32
    DraIII-HF®CutSmart® Buffer≥2,000≥1,000
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    EagI (1)NEBuffer 3.1250
    EagI-HF®CutSmart® Buffer5002
    EcoRI (1) (2)NEBuffer EcoRI250
    EcoRI-HF® (2)CutSmart® Buffer16,00064
    EcoRV (1) (2)NEBuffer 3.11,000
    EcoRV-HF® (2)CutSmart® Buffer≥64,000≥64
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    HindIII (1)NEBuffer 2.1250
    EnzymeBufferMax Units Without Star ActivityHF Factor
    HindIII-HF®CutSmart® Buffer≥500,000≥2,000
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    KpnI (1) (2)NEBuffer 1.116
    KpnI-HF® (2)CutSmart® Buffer≥1,000,000≥62,500
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    MfeI (1) (2)CutSmart® Buffer32
    MfeI-HF® (2)CutSmart® Buffer≥500≥16
    MluI-HF®CutSmart® Buffer
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    NcoI (1) (2)NEBuffer 3.1120
    NcoI-HF® (2)CutSmart® Buffer≥64,000≥530
    NheI (1) (2)NEBuffer 2.1120
    NheI-HF® (2)CutSmart® Buffer≥32,000≥266
    NotI (1) (2)NEBuffer 3.14,000
    NotI-HF® (2)CutSmart® Buffer≥64,000≥16
    NruI-HF®CutSmart® Buffer
    NsiI-HF®CutSmart® Buffer
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    PstI (1)NEBuffer 3.1120
    PstI-HF®CutSmart® Buffer4,00033
    PvuI (1)NEBuffer 3.1500
    PvuI-HF®CutSmart® Buffer≥16,000≥32
    PvuII (1)NEBuffer 3.116
    PvuII-HF®CutSmart® Buffer50032
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    SacI (1)NEBuffer 1.1120
    SacI-HF®CutSmart® Buffer≥32,000≥266
    SalI (1) (2)NEBuffer 3.14
    SalI-HF® (2)CutSmart® Buffer≥32,000≥8,000
    SbfI (1)CutSmart® Buffer8
    SbfI-HF®CutSmart® Buffer25032
    ScaI (1) (2)NEBuffer 34
    ScaI-HF® (2)CutSmart® Buffer25062
    SpeI (1) (2)CutSmart® Buffer
    SpeI-HF®CutSmart® Buffer
    SphI (1)NEBuffer 2.132
    SphI-HF®CutSmart® Buffer8,000250
    SspI (1)NEBuffer SspI32
    SspI-HF®CutSmart® Buffer50016
    StyI (1)NEBuffer 3.132
    StyI-HF®CutSmart® Buffer4,000125

    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.
    † Wild type enzymes were tested in supplied buffer for comparisons.
    * Wei, H. et al. (2008) Nucleic Acids Res. 36, e50.

    HF® is a registered trademark of New England Biolabs, Inc.