Reduced Star Activities of HF® Enzymes

The following table indicates the number of units of HF®-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.

Enzyme Buffer Max Units Without Star Activity HF Factor
AgeI § NEBuffer™ 1.1 32
AgeI-HF® CutSmart® Buffer ≥250 ≥8
ApoI § NEBuffer™ 3.1 20
ApoI-HF CutSmart® Buffer 500 25
Enzyme Back to top NEBuffer Sequence Properties
BamHI § NEBuffer™ 3.1 32
BamHI-HF® CutSmart® Buffer ≥4,000 ≥125
BbsI § NEBuffer™ 2.1 120
BbsI-HF® CutSmart® Buffer ≥500 ≥4
BmtI § NEBuffer™ 3.1 32
BmtI-HF® CutSmart® Buffer 1000000 62500
BsaI § CutSmart® Buffer 32
BsaI-HF® CutSmart® Buffer ≥8,000 ≥250
BsiWI § NEBuffer™ 3.1 100
BsiWI-HF® CutSmart® Buffer 100 1
BsrGI § NEBuffer™ 2.1 16 ≥62
BsrGI-HF® CutSmart® Buffer ≥2000
BstEII  § NEBuffer™ 3.1 16
BstEII-HF® CutSmart® Buffer >2000 >125
BstZ17I-HF® CutSmart® Buffer 500 25
Enzyme Back to top NEBuffer Sequence Properties
DraIII-HF® CutSmart® Buffer ≥2,000 ≥1,000
Enzyme Back to top NEBuffer Sequence Properties
EagI § NEBuffer™ 3.1 250
EagI-HF® CutSmart® Buffer 500 2
EcoRI § NEBuffer™ EcoRI 250
EcoRI-HF® CutSmart® Buffer 16,000 64
EcoRV § NEBuffer™ 3.1 1,000
EcoRV-HF® CutSmart® Buffer ≥64,000 ≥64
Enzyme Back to top NEBuffer Sequence Properties
HindIII § NEBuffer™ 2.1 250
HindIII-HF® CutSmart® Buffer ≥500,000 ≥2,000
Enzyme Back to top NEBuffer Sequence Properties
KpnI § NEBuffer™ 1.1 16
KpnI-HF® CutSmart® Buffer ≥1,000,000 ≥62,500
Enzyme Back to top NEBuffer Sequence Properties
MfeI § CutSmart® Buffer 32
MfeI-HF® CutSmart® Buffer ≥500 ≥16
MluI § NEBuffer™ 3.1 ≥2000
MluI-HF® CutSmart® Buffer ≥4000 2
Enzyme Back to top NEBuffer Sequence Properties
NcoI § NEBuffer™ 3.1 120
NcoI-HF® CutSmart® Buffer ≥64,000 ≥530
NheI § NEBuffer™ 2.1 120
NheI-HF® CutSmart® Buffer ≥32,000 ≥266
NotI § NEBuffer™ 3.1 4,000
NotI-HF® CutSmart® Buffer ≥64,000 ≥16
NruI § NEBuffer™ 3.1 ≥500
NruI-HF® CutSmart® Buffer ≥32000 64
NsiI § NEBuffer™ 3.1 ≥4000
NsiI-HF® CutSmart® Buffer ≥8000 2
Enzyme Back to top NEBuffer Sequence Properties
PstI § NEBuffer™ 3.1 120
PstI-HF® CutSmart® Buffer 4,000 33
PvuI § NEBuffer™ 3.1 500
PvuI-HF® CutSmart® Buffer ≥16,000 ≥32
PvuII § NEBuffer™ 3.1 16
PvuII-HF® CutSmart® Buffer 500 32
Enzyme Back to top NEBuffer Sequence Properties
SacI § NEBuffer™ 1.1 120
SacI-HF® CutSmart® Buffer ≥32,000 ≥266
SalI § NEBuffer™ 3.1 4
SalI-HF® CutSmart® Buffer ≥32,000 ≥8,000
SbfI § CutSmart® Buffer 8
SbfI-HF® CutSmart® Buffer 250 32
ScaI-HF® CutSmart® Buffer 250 62
SpeI § CutSmart® Buffer 500
SpeI-HF® CutSmart® Buffer ≥8000 ≥16
SphI § NEBuffer™ 2.1 32
SphI-HF® CutSmart® Buffer 8,000 250
SspI-HF® CutSmart® Buffer 500 16
StyI § NEBuffer™ 3.1 32
StyI-HF® CutSmart® Buffer 4,000 125

§ A HF version of this enzyme is available.
† Wild type enzymes were tested in supplied buffer for comparisons.
* Wei, H. et al. (2008) Nucleic Acids Res. 36, e50.

 

HF® is a registered trademark of New England Biolabs, Inc.