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    • Reduced Star Activities of HF® Enzymes

    The following table indicates the number of units of HF®-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.

    EnzymeBufferMax Units Without Star ActivityHF Factor
    AgeINEBuffer 1.132
    AgeI-HFCutSmart™ Buffer≥250≥8
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    BamHINEBuffer 3.132
    BamHI-HF®CutSmart™ Buffer≥4,000≥125
    BmtINEBuffer 3.132
    BmtI-HF®CutSmart™ Buffer100000062500
    BsaICutSmart™ Buffer32
    BsaI-HF®CutSmart™ Buffer≥8,000≥250
    BstEII NEBuffer 3.116
    BstEII-HF®CutSmart™ Buffer>2000>125
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    DraIIINEBuffer 3 + BSA2
    DraIII-HF®CutSmart™ Buffer≥2,000≥1,000
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    EagINEBuffer 3.1250
    EagI-HF®CutSmart™ Buffer5002
    EcoRINEBuffer EcoRI250
    EcoRI-HF®CutSmart™ Buffer16,00064
    EcoRVNEBuffer 3.11,000
    EcoRV-HF®CutSmart™ Buffer≥64,000≥64
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    HindIIINEBuffer 2.1250
    HindIII-HF®CutSmart™ Buffer≥500,000≥2,000
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    KpnINEBuffer 1.116
    KpnI-HF®CutSmart™ Buffer≥1,000,000≥62,500
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    MfeICutSmart™ Buffer32
    MfeI-HF®CutSmart™ Buffer≥500≥16
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    NcoINEBuffer 3.1120
    NcoI-HF®CutSmart™ Buffer≥64,000≥530
    NheINEBuffer 2.1120
    NheI-HF®CutSmart™ Buffer≥32,000≥266
    NotINEBuffer 3.14,000
    NotI-HF®CutSmart™ Buffer≥64,000≥16
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    PstINEBuffer 3.1120
    PstI-HF®CutSmart™ Buffer4,00033
    PvuINEBuffer 3.1500
    PvuI-HF®CutSmart™ Buffer≥16,000≥32
    PvuIINEBuffer 3.116
    PvuII-HF®CutSmart™ Buffer50032
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    SacINEBuffer 1.1120
    SacI-HF®CutSmart™ Buffer≥32,000≥266
    SalINEBuffer 3.14
    SalI-HF®CutSmart™ Buffer≥32,000≥8,000
    SbfICutSmart™ Buffer8
    SbfI-HF®CutSmart™ Buffer25032
    ScaINEBuffer 34
    ScaI-HF®CutSmart™ Buffer25062
    SpeICutSmart™ Buffer
    SpeI-HF®CutSmart™ Buffer
    SphI-HF®CutSmart™ Buffer8,000250
    SphINEBuffer 2.132
    SspINEBuffer SspI32
    SspI-HF®CutSmart™ Buffer50016
    StyINEBuffer 3.132
    StyI-HF®CutSmart™ Buffer4,000125

    † Wild type enzymes were tested in supplied buffer for comparisons.
    * Wei, H. et al. (2008) Nucleic Acids Res. 36, e50.

    HF® is a trademark of New England Biolabs, Inc.