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    • Reduced Star Activities of HF® Enzymes

    The following table indicates the number of units of HF®-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.

    EnzymeBufferMax Units Without Star ActivityHF Factor
    AgeINEBuffer 1.132
    AgeI-HF™CutSmart Buffer≥250≥8
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    BamHINEBuffer 3.132
    BamHI-HF™CutSmart Buffer≥4,000≥125
    BmtINEBuffer 3.116
    BmtI-HF™CutSmart Buffer100000062500
    BsaICutSmart Buffer32
    BsaI-HF™CutSmart Buffer≥8,000≥250
    BstEII NEBuffer 3.116
    BstEII-HF™CutSmart Buffer>2000>125
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    DraIIINEBuffer 3 + + BSA2
    DraIII-HF™CutSmart Buffer≥2,000≥1,000
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    EagINEBuffer 3.1250
    EagI-HF™CutSmart Buffer5002
    EcoRINEBuffer EcoRI250
    EcoRI-HF™CutSmart Buffer16,00064
    EcoRVNEBuffer 3.11,000
    EcoRV-HF™CutSmart Buffer≥64,000≥64
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    HindIIINEBuffer 2.1250
    HindIII-HF™CutSmart Buffer≥500,000≥2,000
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    KpnINEBuffer 1.116
    KpnI-HF™CutSmart Buffer≥1,000,000≥62,500
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    MfeICutSmart Buffer32
    MfeI-HF™CutSmart Buffer≥500≥16
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    NcoINEBuffer 3.1120
    NcoI-HF™CutSmart Buffer≥64,000≥530
    NheINEBuffer 2.1120
    NheI-HF™CutSmart Buffer≥32,000≥266
    NotINEBuffer 3.14,000
    NotI-HF™CutSmart Buffer≥64,000≥16
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    PstINEBuffer 3.1120
    PstI-HF™CutSmart Buffer4,00033
    PvuINEBuffer 3.1500
    PvuI-HF™CutSmart Buffer≥16,000≥32
    PvuIINEBuffer 3.116
    PvuII-HF™CutSmart Buffer50032
    EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
    SacINEBuffer 1.1120
    SacI-HF™CutSmart Buffer≥32,000≥266
    SalINEBuffer 3.14
    SalI-HF™CutSmart Buffer≥32,000≥8,000
    SbfICutSmart Buffer8
    SbfI-HF™CutSmart Buffer25032
    ScaINEBuffer 34
    ScaI-HF™CutSmart Buffer25062
    SpeICutSmart Buffer
    SpeI-HF™CutSmart Buffer
    SphI-HF™CutSmart Buffer8,000250
    SphINEBuffer 2.132
    SspINEBuffer SspI32
    SspI-HF™CutSmart Buffer50016
    StyINEBuffer 3.132
    StyI-HF™CutSmart Buffer4,000125

    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.
    † Wild type enzymes were tested in supplied buffer for comparisons.
    * Wei, H. et al. (2008) Nucleic Acids Res. 36, e50.

    HF® is a registered trademark of New England Biolabs, Inc.