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  • Chart Legend

    U

    Supplied with its own unique reaction buffer that is different from the four standard NEBuffers. Its compatibility with the four standard NEBuffers is indicated by the chart.

    BSA

    Supplied with a separate vial of bovine serum albumin (10 mg/ml). To obtain 100% activity BSA should be added to the 1X reaction mix to a final concentration of 100 µg/ml.

    SAM

    Supplied with a separate vial of S-adenosyl-methionine (SAM). To obtain 100% activity, SAM should be added to the 1X reaction mix as specified on the product data card.

    dd

    When performing a double digest with this enzyme, this NEBuffer is recommended because it minimizes star activity.

    *

    Purified by scientists at SibEnzyme and supplied with a SibEnzyme buffer (B, K, O, W or Y) which ensures 100% activity. Its compatibility with the NEBuffer System is indicated on the chart.

    N/R

    This buffer is not recommended for use with with this enzyme.

    Buffer Compositions (1X):

    NEBuffer 1:
    10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).

    NEBuffer 2:
    10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

    NEBuffer 3:
    50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

    NEBuffer 4:
    20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).