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  • McrA, McrBC and EcoKI Strain Phenotypes

      Mcr Mcr EcoK Strain Background
    Strain A BC R M
    1100 + + + +  
    AB266 + + + +  
    AB1157 + + + + Mrr+
    AT2459 - + + +  
    BNN93 - - - + C600
    BNN102 - - - + C600
    CH731* + ? ? ?  
    CH1332* - ? ? ? CH731
    CH1372* - ? ? ? CH731
    C235 - + + +  
    C600 *** + + + Y70; Mrr+
    CES200 - + - + AB1157
    CJ236** (#E4141S) - + + +  
    CPB1293 - + + + W3110
    CPB1321 - - - + W3110
    CR63 - + + +  
    CSR603 ? + + +  
    c2813 - - - + WA802; Mrr+
    DB1316* + - - + Mrr+
    DH1 + + - + MM294; Mrr+
    DH3 - + - + MM294; Mrr+
    DH5 + + - + MM294; Mrr+
    DH5aMCR* - Δ Δ Δ DH5; Mrr -
    DH10B* - Δ Δ Δ MC1061; Mrr -
    DL538* - - - + NM621
    DS410* - + + +  
    DM800 - + + +  
    ED8641 - + - + WA803
    ED8654 - + - + WA803
    ED8739 - - - - WA803
    ED8767 - - - - WA803
    ER1370 + + + + NK7254 (JC1552)
    ER1381 + + - + ER1370; Mrr+
    ER1378 + - - + ER1370; Mrr+
    ER1398 + - - + MM294; Mrr+
    ER1414 + - - + W3110; Mrr+
    ER1458 - - - + Y1084
    ER1562 - - - + MM294; Mrr+
    ER1563 - + - + MM294; Mrr+
    ER1564 - + - + ER1370; Mrr+
    ER1565 - - - + ER1370; Mrr+
    ER1647* - Δ Δ Δ ER1370; Mrr -
    ER1648 - Δ Δ Δ ER1370; Mrr -
    ER1793*(#E4101S) - Δ Δ Δ ER1370; Mrr -
    ER1821** (#E4102S) - Δ Δ Δ MM294; Mrr -
    ER2267** (#E4103S) - Δ Δ Δ MM294; F+; Mrr -
    ER2925(#E4109S) - - - + GM271; Mrr +
    FS1585 - + + + C600
    GM31* - +/- + + Mrr+/-
    GM161 ? + + +  
    GM271 - - - + Mrr+
    GM272 ? + + +  
    GM2159** + + + + Mrr+
    GM2929** - - - + GM2163 recF
    GW1002 - ? + +  
    H680 - ? + +  
    HB101 + Δ Δ Δ Mrr -
    Hfr3000 YA149 - + + + HfrH
    Hfr3000 - + + +  
    Hfr Cavalli + - + +  
    Hfr Hayes** + + + + Mrr+
    HfrH thi - - + + +  
    HfrP4X6 + + + +  
    HfrR4** + + + + Mrr+
    HL1228* - - - + NM621
    JC7623** - + + + Mrr+
    JH132 - Δ Δ Δ K802
    JM83 (+) + + +  
    JM101 + (+) + + JM83
    JM107 - + - + MM294; Mrr+
    JM107MA2 - - - + JM107
    JM109 (-) (+) - + JM107
    5K - + - + C600
    K12 + + + +  
    K802 see WA802  
    K803 see WA803  
    K10 + - + + Hfr Cavalli
    KMBL1164 - ? + +  
    KW251* - - - +  
    LE392 - + - + ED8654
    MB406* - + - +  
    MC1040 (+) (+)  
    MC1061 - - - + Mrr+
    MM294 + + - + 1100; Mrr+
    NM477 - - - - C600
    NM494 - + - - pop101
    NM514 - &/or + - + pop101
    NM519 + + - +  
    NM538 (-) (+) - + ED8654
    NM539 - + - + NM538
    NM554 - - - + MC1061
    NM621 - - - + C600
    NM646* - - - + WA802
    NM664* - Δ Δ Δ WA802; Mrr -
    NM670* - + + + WA802
    NM673* - - - + WA802
    NM674* - Δ Δ Δ WA802; Mrr -
    NW1* - - - + AT2459
    NW2* - Δ Δ Δ AT2459; Mrr -
    P678** + ? ? +  
    PA309 + + + +  
    PC0543** + - + + Mrr+
    PC0950 + - + + Mrr+
    Q358 - + - +  
    Q359 - + - +  
    RL88 - - + +  
    RR1 + Δ Δ Δ HB101; Mrr -
    SK5022 - + + +  
    TC410* - Δ Δ Δ DS410; Mrr -
    TC600* - - - + C600B
    W6 + + + +  
    W677** + ? + +  
    W3110 + + + + Mrr+
    W4797 + + + +  
    WA704 - - + + C600 X HfrCavalli
    WA802 - - - + WA704; Mrr+
    WA803 - - - - WA704; Mrr+
    WW3352 - + + +  
    X149 - + + +  
    Y10 + + + +  
    Y53 + + + + Y10
    Y70 + + + + Y53
    Y1084 - + + + BNN96; SG1041
    Y1088 - + - + LE392; ED8654
    Y1090 (-) (+) + + Y1084
    Non-K12 strains
    E. coli B + + + +  
    E. coli B/r ? + + +  
    BL21** + + - - restricts l.Sss I
    E. coli C - (-) - -  

    Mcr phenotypes reported in [Raleigh, E.A. et al. (1988) Nucl. Acids Res., 16, 1563-1575 PMID: 2831502, References 7 in Restriction of Foreign DNA by E. coli K-12] except those denoted with asterisks. Mrr phenotypes determined at NEB except DH10B (11). EcoKI phenotypes sometimes inferred from genotype.

    Table Notes:

    • ( ) phenotype inferred from that of ancestral or descendant strains.
    • ? phenotype not tested.
    • Δ mutation present is known to be a large deletion.
    • ‡ These strains were formerly thought to carry the mcrB allele present in E.coli B. They are now known to carry an extensive deletion of mrr -hsd -mcrBC (ref. 6; M. Noyer-Weidner, personal communication; J. Kelleher, personal communication).
    • * Phenotypes reported in one of references 8-12.
    • ** Phenotypes determined at New England Biolabs, Inc.
    • Orange catalog numbers in paretheses indicate strains that are available upon request from New England Biolabs at no charge with an order or for the cost of shipping if ordered separately.
    • *** C600 lines obtained from different sources give different results. The original strain, and that obtained from the E. coli Genetic Stock Center (Yale University) are McrA+; derivatives traceable to the Brenner laboratory are McrA- (21).