Convenient Formats of Competent Cells

NEB provides these superior competent cell strains in several formats for your convenience. All are available as 50 µl single-use transformation tubes and many are available in larger, 200 µl tubes for multiple simultaneous reactions. Our most popular cloning strains are available as electrocompetent cells. NEB 5-alpha is also available in a lower efficiency, subcloning format for substantial value.

Cloning Formats:
Formats	NEB #	Single-use	200 µl tubes	96-well plate	Electrocompetent	Subcloning	SOC & Control Plasmid Included
NEB Turbo	C2984	✓	✓		✓	–	✓
NEB 5-alpha	C2987	✓	✓	✓	✓	✓	✓
NEB 5-alpha F'Iq	C2992	✓	✓		–	–	✓
NEB 10-beta	C3019	✓	✓		✓	–	✓
dam-/dcm-	C2925	✓	✓		–	–	✓
Protein Expression Formats:
NEB Express	C2523	✓	✓		–	–	✓
NEB Express Iq	C3037	✓	✓		–	–	✓
T7 Express	C2566	✓	✓		–	–	✓
T7 Express Iq	C3016	✓	✓		–	–	✓
T7 Express lysY	C3010	✓	✓		–	–	✓
T7 Express lysY/Iq	C3013	✓	✓		–	–	✓
T7 Express Crystal	C3022	✓	✓		–	–	✓
SHuffle Express	C3028	✓	–		–	✓	–
SHuffle T7	C3026	✓	–		–	–	–
SHuffle T7 Express lysY	C3030	✓	–		–	✓	–
SHuffle T7 Express	C3029	✓	–		–	✓	–
BL21	C2530	✓	–		–	–	✓
BL21(DE3)	C2527	✓	✓		–	–	✓
NiCo21(DE3)	C2529	✓	–		–	–	✓
Lemo21(DE3)	C2528	✓	–		–	–	L-rhamnose and Control Plasmid