Convenient Formats of Competent Cells
NEB provides these superior competent cell strains in several formats for your convenience. All are available as 50 µl single-use transformation tubes and many are available in larger, 200 µl tubes for multiple simultaneous reactions. Our most popular cloning strains are available as electrocompetent cells. NEB 5-alpha is also available in a lower efficiency, subcloning format for substantial value.
96-well, 384-well and StripWell formats may be customized upon request. Please inquire.
| Cloning Formats: | |||||||
|---|---|---|---|---|---|---|---|
| Formats | NEB # | Single-use | 200 µl tubes | 96-well plate | Electrocompetent | Subcloning | SOC & Control Plasmid Included |
| NEB Turbo | C2984 | ✓ | ✓ | ✓ | – | ✓ | |
| NEB 5-alpha | C2987 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| NEB 5-alpha F'Iq | C2992 | ✓ | ✓ | – | – | ✓ | |
| NEB 10-beta | C3019 | ✓ | ✓ | ✓ | – | ✓ | |
| dam-/dcm- | C2925 | ✓ | ✓ | – | – | ✓ | |
| Protein Expression Formats: | |||||||
| NEB Express | C2523 | ✓ | ✓ | – | – | ✓ | |
| NEB Express Iq | C3037 | ✓ | ✓ | – | – | ✓ | |
| T7 Express | C2566 | ✓ | ✓ | – | – | ✓ | |
| T7 Express Iq | C3016 | ✓ | ✓ | – | – | ✓ | |
| T7 Express lysY | C3010 | ✓ | ✓ | – | – | ✓ | |
| T7 Express lysY/Iq | C3013 | ✓ | ✓ | – | – | ✓ | |
| T7 Express Crystal | C3022 | ✓ | ✓ | – | – | ✓ | |
| SHuffle Express | C3028 | ✓ | – | – | ✓ | – | |
| SHuffle T7 | C3026 | ✓ | – | – | – | – | |
| SHuffle T7 Express lysY | C3030 | ✓ | – | – | ✓ | – | |
| SHuffle T7 Express | C3029 | ✓ | – | – | ✓ | – | |
| BL21 | C2530 | ✓ | – | – | – | ✓ | |
| BL21(DE3) | C2527 | ✓ | ✓ | – | – | ✓ | |
| NiCo21(DE3) | C2529 | ✓ | – | – | – | ✓ | |
| Lemo21(DE3) | C2528 | ✓ | – | – | – | L-rhamnose and Control Plasmid | |
