Time-Saver™ Qualified Enzymes

Recombinant Digest in 5 minutes
Enzymes that are Time-Saver qualified will digest unit assay substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions Digest in 15 minutes
Not completely digested in 15 minutes
NT Not tested.

Enzyme Sequence Recombinant Time-Saver Qualified Unit Assay Substrate Plasmid Substrate PCR Substrate
AatII GACGT/C    
AccI GT/MKAC    
Acc65I G/GTACC    
AciI CCGC(-3/-1)    
AclI AA/CGTT    
AcuI CTGAAG(16/14)    
AflII C/TTAAG    
AgeI-HF® A/CCGGT    
AhdI GACNNN/NNGTC    
AluI AG/CT    
AlwNI CAGNNN/CTG    
ApaI GGGCC/C    
ApaLI G/TGCAC    
ApeKI G/CWGC    
ApoI § R/AATTY    
ApoI-HF R/AATTY    
AscI GG/CGCGCC     NT
AseI AT/TAAT     NT
AvaI C/YCGRG    
AvaII G/GWCC    
AvrII C/CTAGG     NT NT
BaeGI GKGCM/C    
BaeI (10/15)ACNNNNGTAYC(12/7)    
BamHI § G/GATCC    
BamHI-HF® G/GATCC    
BbsI § GAAGAC(2/6)    
BbsI-HF® GAAGAC(2/6)    
BbvI GCAGC(8/12)    
BccI CCATC(4/5)  
BceAI ACGGC(12/14)  
BciVI GTATCC(6/5)    
BclI T/GATCA    
BclI-HF T/GATCA    
BcoDI GTCTC(1/5)    
BfuAI ACCTGC(4/8)    
BfuCI /GATC  
BglI GCCNNNN/NGGC    
BglII A/GATCT    
BlpI GC/TNAGC    
BmgBI CACGTC(-3/-3)    
BmrI ACTGGG(5/4)  
BmtI-HF® GCTAG/C    
BpuEI CTTGAG(16/14)    
BsaAI YAC/GTR    
BsaHI GR/CGYC    
BsaI-HF® GGTCTC(1/5)    
BsaWI W/CCGGW    
BsaXI (9/12)ACNNNNNCTCC(10/7)  
BseRI GAGGAG(10/8)    
BsgI GTGCAG(16/14)    
BsiEI CGRY/CG    
BsiWI § C/GTACG    
BsiWI-HF® C/GTACG    
BslI CCNNNNN/NNGG    
BsmAI GTCTC(1/5)    
BsmBI CGTCTC(1/5)    
BsmFI GGGAC(10/14)  
BsmI GAATGC(1/-1)    
BsoBI C/YCGRG    
BspCNI CTCAG(9/7)    
BspEI T/CCGGA    
BspHI T/CATGA    
Bsp1286I GDGCH/C    
BspQI GCTCTTC(1/4)    
BsrBI CCGCTC(-3/-3)    
BsrDI GCAATG(2/0)    
BsrFαI R/CCGGY    
BsrGI § T/GTACA    
BsrGI-HF® T/GTACA    
BsrI ACTGG(1/-1)  
BssHII G/CGCGC    
BssSαI CACGAG(-5/-1)    
BstBI TT/CGAA    
BstEII  § G/GTNACC    
BstEII-HF® G/GTNACC    
BstNI CC/WGG    
BstUI CG/CG  
BstXI CCANNNNN/NTGG    
BstYI R/GATCY    
BstZ17I-HF® GTATAC    
Bsu36I CC/TNAGG    
BtgI C/CRYGG    
BtsαI GCAGTG(2/0)    
BtsCI GGATG(2/0)    
Cac8I GCN/NGC  
ClaI AT/CGAT    
CspCI (11/13)CAANNNNNGTGG(12/10)    
CviAII C/ATG    
CviQI G/TAC    
DdeI C/TNAG    
DpnI GA/TC    
DpnII /GATC    
DraI TTT/AAA    
DraIII-HF® CACNNN/GTG    
DrdI GACNNNN/NNGTC  
EagI § C/GGCCG    
EagI-HF® C/GGCCG    
EarI CTCTTC(1/4)    
Eco53kI GAG/CTC    
EcoNI CCTNN/NNNAGG    
EcoO109I RG/GNCCY    
EcoP15I CAGCAG(25/27)    
EcoRI § G/AATTC    
EcoRI-HF® G/AATTC    
EcoRV § GAT/ATC    
EcoRV-HF® GAT/ATC    
Fnu4HI GC/NGC    
FokI GGATG(9/13)  
FseI GGCCGG/CC    
FspI TGC/GCA    
HaeII RGCGC/Y    
HaeIII GG/CC    
HgaI GACGC(5/10)  
HhaI GCG/C    
HincII GTY/RAC    
HindIII-HF® A/AGCTT    
HinfI G/ANTC    
HinP1I G/CGC    
HpaII C/CGG    
HphI GGTGA(8/7)    
HpyAV CCTTC(6/5)     NT
HpyCH4IV A/CGT    
HpyCH4V TG/CA    
Hpy166II GTN/NAC    
KpnI § GGTAC/C  
KpnI-HF® GGTAC/C    
MboI /GATC    
MboII GAAGA(8/7)  
MfeI § C/AATTG  
MfeI-HF® C/AATTG    
MluCI /AATT    
MluI § A/CGCGT    
MlyI GAGTC(5/5)    
MmeI TCCRAC(20/18)    
MnlI CCTC(7/6)    
MseI T/TAA    
MslI CAYNN/NNRTG    
MspA1I CMG/CKG    
MspI C/CGG    
MwoI GCNNNNN/NNGC    
NciI CC/SGG    
NcoI § C/CATGG    
NcoI-HF® C/CATGG    
NdeI CA/TATG    
NgoMIV G/CCGGC    
NheI § G/CTAGC    
NheI-HF® G/CTAGC    
NlaIII CATG/    
NmeAIII GCCGAG(21/19)  
NotI § GC/GGCCGC    
NotI-HF® GC/GGCCGC    
NruI § TCG/CGA    
NsiI § ATGCA/T    
NspI RCATG/Y    
PacI TTAAT/TAA    
PaeR7I C/TCGAG    
PflFI GACN/NNGTC    
PflMI CCANNNN/NTGG    
PmeI GTTT/AAAC     NT
PmlI CAC/GTG    
PpuMI RG/GWCCY    
PshAI GACNN/NNGTC    
PstI § CTGCA/G    
PstI-HF® CTGCA/G    
PvuI § CGAT/CG    
PvuI-HF® CGAT/CG    
PvuII § CAG/CTG    
PvuII-HF® CAG/CTG    
RsaI GT/AC    
SacI § GAGCT/C    
SacI-HF® GAGCT/C    
SacII CCGC/GG    
SalI § G/TCGAC    
SalI-HF® G/TCGAC    
SapI GCTCTTC(1/4)    
SbfI § CCTGCA/GG    
SbfI-HF® CCTGCA/GG    
ScaI-HF® AGT/ACT    
SfiI GGCCNNNN/NGGCC    
SfoI GGC/GCC    
SmaI CCC/GGG    
SpeI § A/CTAGT    
SpeI-HF® A/CTAGT    
SphI-HF® GCATG/C    
SrfI GCCC/GGGC    
SspI § AAT/ATT    
SspI-HF® AAT/ATT    
StuI AGG/CCT    
StyD4I /CCNGG    
StyI § C/CWWGG    
StyI-HF® C/CWWGG    
SwaI ATTT/AAAT    
TaqαI T/CGA    
TfiI G/AWTC    
TseI G/CWGC  
TspMI C/CCGGG  
TspRI NNCASTGNN/    
Tth111I GACN/NNGTC    
XbaI T/CTAGA    
XhoI C/TCGAG    
XmaI C/CCGGG    
XmnI GAANN/NNTTC    

§ An HF version of this enzyme is available.
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