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  • Thermophilic DNA Polymerases

    NEB supplies many thermophilic DNA polymerases; several characteristics should be considered when choosing the one best suited to your particular research needs.

    VentR® VentR® (exo-) Deep VentR Deep VentR™ (exo-) Taq 9°N™ Phusion®** Long Amp® Taq Long Amp® Hot Start Taq One Taq® Q5™
    Exonuclease Activities
    proofreading exonuclease yes no yes no no no* yes yes yes yes yes
    5'—>3' exonuclease activity no no no no yes no no yes yes yes no
    Thermal Stability
    Half life at 95°C 6.7 hours 6.7 hours 23 hours 23 hours 0.9 hours 6.7 hours >6 hours at 96°C ND ND ND ND
    Half life at 100°C 1.8 hours 1.8 hours 8 hours 8 hours <0.1 hours <1.8 hours 2 hours at 98°C ND ND ND ND
    Primer Extension Characteristics
    Units/100 µl Primer Extension Rxn 0.4-2 units 1-4units 0.4-2 units 1-4 units 2.5-5 units 1-2 units 1-2 units 2-10 units 2-10 units 2.5-5 units 1-2 units
    Fidelity to Taq 5X 2X 6X 2X 1X ND >50X 2X 2X 2X >100X
    Resulting DNA Ends blunt 3´ A blunt 3´ A 3´ A 3´ A blunt blunt 3´ A
    blunt
    3´ A
    blunt
    3´ A
    blunt
    blunt
    Strand Displacement yes; temp. dependent yes; temp. dependent yes; temp. dependent yes; temp. dependent no yes no yes; temp. dependent yes; temp. dependent yes; temp. dependent no
    Longest Primer Extension to Date 13.2 kb 15.0 kb 14.0 kb 15.0 kb 8.1 kb 37 kb 40 kb 40 kb 15.0 kb 20 kb 20 kb

    VentR®, LongAmp® and OneTaq® are registered trademarks of New England Biolabs, Inc.
    Deep VentR™, 9°N™ and Q5™ are trademarks of New England Biolabs, Inc.

    * 1-5% of wild type exonuclease activity is present in the modified enzyme.

    ** Notice to Customers:
    Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. Under agreement with, and under the performance specifications of Thermo Fisher Scientific.