Cloning vectors are available for SNAP-tag and CLIP-tag fusion protein expression and labeling in mammalian and bacterial systems. The mammalian SNAPf and CLIPf vectors express faster-reacting variants of the SNAP- and CLIP-tags than previously available vectors. Improved polylinker sequences both upstream and downstream from the tag allow expression of the tag on either end of the protein of interest, under control of the CMV promoter. SNAPf-tag and CLIPf-tag expression vectors contain a neomycin resistance (NeoR) gene for selection of stable transfectants, together with an IRES element for efficient expression of both the fusion protein and NeoR. Codon usage has been optimized for mammalian expression. Control plasmids encoding fusion proteins that are localized to the nucleus (H2B), mitochondria (Cox8A) and cell surface (ADRβ2, NK1R, GPI) are also available through Addgene (addgene.org/New_England_Biolabs/). The pSNAP-tag® (T7)-2 Vector (NEB #N9322) is an Escherichia coli expression plasmid encoding the SNAP-tag protein. The codon usage of the SNAP26b gene is optimized for expression in E. coli. Expression is under control of the IPTG inducible T7 promoter.
|pSNAPf Vector||N9183||Stable and transient mammalian expression||20 μg|
|pSNAP-tag® (T7)-2 Vector||N9181||Bacterial expression under T7 control||20 μg|
|pCLIPf Vector||N9215||Stable and transient mammalian expression||20 μg|