SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart

NEB® offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker, while CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. These substrates will label their respective tags without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell® and CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface® and CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.

ACP/MCP-tag substrates are conjugated to the phosphopantetheinyl moeity of Coenzyme A (CoA) and require a separate enzyme (ACP or SFP Synthase) for labeling. The labeling reaction is specific for fusion proteins expressed on the cell surface. The same substrates are used to label both the ACP- and the MCP-tags; specificity is determined by the synthase used for labeling.

Self-Labeling Tags

SNAP-tag Cell-Permeable

Substrates NEB # Excitation* Emission* ✝ Size
SNAP-Cell® 647-SiR #S9102 645 661 30 nmol
SNAP-Cell® 505-Star #S9103 504 532
50 nmol
SNAP-Cell® Oregon Green® #S9104 490 514
50 nmol
SNAP-Cell® Fluorescein #S9107 500 532
50 nmol
SNAP-Cell® 430 #S9109 421 444484
50 nmol
SNAP-Cell® TMR-Star #S9105 554 580
30 nmol

Non-cell-permeable

Substrates NEB # Excitation* Emission* ✝ Size
SNAP-Surface® 649 #S9159 0 50 nmol
SNAP-Surface® 594 #S9134 606 626
50 nmol
SNAP-Surface® 488 #S9124 506 526
50 nmol
SNAP-Surface® 549 #S9112 560 575
50 nmol
SNAP-Surface® Alexa Fluor® 546 #S9132 558 574
50 nmol
SNAP-Surface® Alexa Fluor® 488 #S9129 496 520
50 nmol
SNAP-Surface® Alexa Fluor® 647 #S9136 652 670
50 nmol

CLIP-tag Cell-Permeable

Substrates NEB # Excitation* Emission* ✝ Size
CLIP-Cell™ 505 #S9217 504 532
50 nmol
CLIP-Cell™ TMR-Star #S9219 554 580
30 nmol

Non-cell-permeable

Substrates NEB # Excitation* Emission* ✝ Size
CLIP-Surface™ 647 #S9234 660 673
50 nmol
CLIP-Surface™ 488 #S9232 506 526
50 nmol
CLIP-Surface™ 547 #S9233 554 568
50 nmol

Enzymatic Tag

ACP/MCP tag Non-cell-permeable

Substrates NEB # Excitation* Emission* ✝ Size

* Excitation and emission values determined experimentally for labeled protein tag.
† Colors are based on the electromagnetic spectrum. Actual color visualization may vary.

SNAP-tag®, SNAP-Cell®, SNAP-Surface® and NEB® are registered tradmarkes of New England Biolabs, Inc.
CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.

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