SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart

NEB® offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker, while CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. These substrates will label their respective tags without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell® and CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface® and CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.

ACP/MCP-tag substrates are conjugated to the phosphopantetheinyl moeity of Coenzyme A (CoA) and require a separate enzyme (ACP or SFP Synthase) for labeling. The labeling reaction is specific for fusion proteins expressed on the cell surface. The same substrates are used to label both the ACP- and the MCP-tags; specificity is determined by the synthase used for labeling.

Self-Labeling Tags

SNAP-tag Cell-Permeable

SubstratesNEB #Excitation*Size
Emission* ✝
SNAP-Cell® 360#S9101357437
50nmol
SNAP-Cell® 505-Star#S9103504532
50nmol
SNAP-Cell® Fluorescein#S9107500532
50nmol
SNAP-Cell® Oregon Green®#S9104490514
50nmol
SNAP-Cell® TMR-Star#S9105554580
30nmol
SNAP-Cell® 430#S9109421444484
50nmol
SNAP-Cell® 647-SiR#S9102645661
0A260 units

Non-cell-permeable

SubstratesNEB #Excitation*Size
Emission* ✝
SNAP-Surface® 425#S9126438489
50nmol
SNAP-Surface® 488#S9124506526
50nmol
SNAP-Surface® 532#S9131536554
50nmol
SNAP-Surface® 549#S9112560575
50nmol
SNAP-Surface® 594#S9134606626
50nmol
SNAP-Surface® 647#S9137660673
50nmol
SNAP-Surface® 649#S91590
50nmol
SNAP-Surface® 682#S9139693708
50nmol
SNAP-Surface® 782#S9142784789
50nmol
SNAP-Surface® Alexa Fluor® 488#S9129496520
50nmol
SNAP-Surface® Alexa Fluor® 546#S9132558574
50nmol
SNAP-Surface® Alexa Fluor® 647#S9136652670
50nmol

CLIP-tag Cell-Permeable

SubstratesNEB #Excitation*Size
Emission* ✝
CLIP-Cell™ 360#S9215357437
50nmol
CLIP-Cell™ 430#S9216421444484
50nmol
CLIP-Cell™ 505#S9217504532
50nmol
CLIP-Cell™ Fluorescein#S9218500532
50nmol
CLIP-Cell™ TMR-Star#S9219554580
30nmol

Non-cell-permeable

SubstratesNEB #Excitation*Size
Emission* ✝
CLIP-Surface™ 488#S9232506526
50nmol
CLIP-Surface™ 547#S9233554568
50nmol
CLIP-Surface™ 647#S9234660673
50nmol

Enzymatic Tag

ACP/MCP tag Non-cell-permeable

SubstratesNEB #Excitation*Size
Emission* ✝
CoA 488#S9348506526
50nmol
CoA 547#S9349554568
50nmol
CoA 647#S9350660673
50nmol

* Excitation and emission values determined experimentally for labeled protein tag.
† Colors are based on the electromagnetic spectrum. Actual color visualization may vary.

SNAP-tag®, SNAP-Cell®, SNAP-Surface® and NEB® are registered tradmarkes of New England Biolabs, Inc.
CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.