NEB® offers a large selection of fluorescent labels (substrates) for SNAP- and CLIP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker, while CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. These substrates will label their respective tags without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell® and CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface® and CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.
|Substrates||NEB #||Excitation*||Emission* ✝||Size|
|SNAP-Surface® 594||#S9134||606||626||50.0 nmol|
|SNAP-Surface® 549||#S9112||560||575||50.0 nmol|
|SNAP-Surface® 488||#S9124||506||526||50.0 nmol|
|SNAP-Surface® 649||#S9159||655||676||50.0 nmol|
|SNAP-Surface® Alexa Fluor® 546||#S9132||558||574||50.0 nmol|
|SNAP-Surface® Alexa Fluor® 647||#S9136||652||670||50.0 nmol|
|SNAP-Surface® Alexa Fluor® 488||#S9129||496||520||50.0 nmol|
* Excitation and emission values determined experimentally for labeled protein tag.
† Colors are based on the electromagnetic spectrum. Actual color visualization may vary.
SNAP-tag®, SNAP-Cell®, SNAP-Surface® and NEB® are registered tradmarkes of New England Biolabs, Inc.
CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.
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