RNA Ligase Selection Chart

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  • While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. Orange boxes indicate new products.

      RNA LIGASES
    T4 RNA
    Ligase 1
    T4 RNA
    Ligase 2
    T4 RNA Ligase 2
    Truncated
    T4 RNA Ligase 2, Truncated K227Q T4 RNA Ligase 2, Truncated KQ Thermostable 5' App
    DNA/RNA Ligase
    5' Adenylation Kit RtcB Ligase
    RNA APPLICATIONS
    Nicks in dsRNA   ★★★            
    Labeling of 3´ termini of RNA ★★★      
    Ligation of ssRNA to ssRNA ★★★              
    Ligation of preadenylated adaptors to RNA ★★   ★★ ★★ ★★★ ★★    
    5´ Adenylation             ★★★  
    Ligation of 3’P and 5’OH of ssRNA             ★★★
    DNA APPLICATIONS
    Ligation of preadenylated adaptors to ssDNA           ★★★  
    DNA/RNA APPLICATIONS
    Joining of RNA & DNA in a ds-structure   ★★            
    Ligation of RNA and DNA with 3’P and 5’OH             ★★
    NGS APPLICATIONS
    NGS Library Prep dsDNA-dsDNA (ligation)                
    NGS Library Prep ssRNA-ssDNA (ligation)        
    NGS Library Prep ssRNA-ds-Adaptor splinted ligation              
    FEATURES
    Thermostable            
    Recombinant  
    KEY
    ★★★
    Optimal, recommended ligase for selected application
    ★★
    Works well for selected application
    Will perform selected application, but is not recommended
    Please consult the specific NGS protocol to determine the optimal enzyme for your needs