RNA Ligase Selection Chart

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  • While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. Orange boxes indicate new products.

      RNA LIGASES
    T4 RNA Ligase 1 T4 RNA Ligase 2 T4 RNA Ligase 2 Truncated T4 RNA Ligase 2, Truncated K227Q T4 RNA Ligase 2, Truncated KQ Thermostable 5' AppDNA/RNA Ligase 5' Adenylation Kit
    RNA APPLICATIONS
    Nicks in dsRNA   ★★★          
    Labeling of 3´ termini of RNA ★★★    
    Ligation of ssRNA to ssRNA ★★★            
    Ligation of preadenylated adaptors to RNA ★★   ★★ ★★ ★★★ ★★  
    5´ Adenylation             ★★★
    DNA APPLICATIONS
    Ligation of preadenylated adaptors to ssDNA           ★★★
    DNA/RNA APPLICATIONS
    Joining of RNA & DNA in a ds-structure   ★★          
    NGS APPLICATIONS
    NGS Library Prep dsDNA-dsDNA (ligation)              
    NGS Library Prep ssRNA-ssDNA (ligation)      
    NGS Library Prep ssRNA-ds-Adaptor splinted ligation            
    FEATURES
    Thermostable          
    Recombinant
    KEY
    ★★★
    Optimal, recommended ligase for selected application
    ★★
    Works well for selected application
    Will perform selected application, but is not recommended
    Please consult the specific NGS protocol to determine the optimal enzyme for your needs