RNA Ligase Selection Chart

While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. Orange boxes indicate new products.

  RNA LIGASES
T4 RNA
Ligase 1
T4 RNA
Ligase 2
T4 RNA Ligase 2
Truncated
T4 RNA Ligase 2, Truncated K227Q T4 RNA Ligase 2, Truncated KQ Thermo-stable 5' App
DNA/RNA Ligase
5' Adeny-lation Kit RtcB Ligase
RNA APPLICATIONS
Nicks in dsRNA   ★★★            
Labeling of 3´ termini of RNA ★★★      
Ligation of ssRNA to ssRNA ★★★              
Ligation of preadenylated adaptors to RNA ★★   ★★ ★★ ★★★ ★★    
5´ Adenylation             ★★★  
Ligation of 3’P and 5’OH of ssRNA             ★★★
DNA APPLICATIONS
Ligation of preadenylated adaptors to ssDNA           ★★★  
DNA/RNA APPLICATIONS
Joining of RNA & DNA in a ds-structure   ★★            
Ligation of RNA and DNA with 3’P and 5’OH             ★★
NGS APPLICATIONS
NGS Library Prep dsDNA-dsDNA (ligation)                
NGS Library Prep ssRNA-ssDNA (ligation)        
NGS Library Prep ssRNA-ds-Adaptor splinted ligation              
FEATURES
Thermostable            
Recombinant  ✓
KEY
★★★
Optimal, recommended ligase for selected application
★★
Works well for selected application
Will perform selected application, but is not recommended
Please consult the specific NGS protocol to determine the optimal enzyme for your needs