RNA Cap Analog Selection Chart

  • My NEB
  • Print
  • PDF
  • The 5′ terminal m7G cap present on most eukaryotic mRNAs promotes translation, in vitro, at the initiation level. For most RNAs, the cap structure increases stability, decreases susceptibility to exonuclease degradation, and promotes the formation of mRNA initiation complexes. Certain prokaryotic mRNAs with 5′ terminal cap structures are translated as efficiently as eukaryotic mRNA in a eukaryotic cell-free protein synthesizing system. Splicing of certain eukaryotic substrate RNAs has also been observed to require a cap structure.

    PRODUCT APPLICATIONS
    Anti-Reverse Cap Analog
    3′-O-Me-m7G(5′) ppp(5′)G
    Produces 100% translatable capped transcripts
    Co-transcriptional capping with T7 (NEB #M0251), SP6 (NEB #M0207) and T3 RNA polymerases
    Synthesis of m7G capped RNA for in vitro splicing assays
    Synthesis of m7G capped RNA for transfection or microinjection
    Standard Cap Analog
    m7G(5′)ppp(5′)G
    Co-transcriptional capping with T7, SP6 and T3 RNA polymerases
    Synthesis of m7G capped RNA for in vitro splicing assays
    Synthesis of m7G capped RNA for transfection or microinjection
    Unmethylated Cap Analog G (5′)ppp(5′)G Co-transcriptional capping with T7, SP6 and T3 RNA polymerases
    Synthesis of unmethylated G capped RNA
    Methylated Cap Analog for A +1 sites
    m7G(5′)ppp(5′)A
    Co-transcriptional capping with T7 RNA polymerase from the phi2.5 promoter that contains an A at the transcription initiation site
    Synthesis of m7G capped RNA for in vitro splicing assays
    Synthesis of m7G capped RNA for transfection or microinjection
    Unmethylated Cap Analog for A +1 sites G(5′)ppp(5′)A Co-transcriptional capping with T7 RNA polymerase from the phi2.5 promoter that contains an A at the transcription initiation site
    Synthesis of unmethylated G capped RNA
    Synthesis of A capped RNA