RNA Cap Analog Selection Chart

The 5′ terminal m7G cap present on most eukaryotic mRNAs promotes translation, in vitro, at the initiation level. For most RNAs, the cap structure increases stability, decreases susceptibility to exonuclease degradation, and promotes the formation of mRNA initiation complexes. Certain prokaryotic mRNAs with 5′ terminal cap structures are translated as efficiently as eukaryotic mRNA in a eukaryotic cell-free protein synthesizing system. Splicing of certain eukaryotic substrate RNAs has also been observed to require a cap structure.

PRODUCT APPLICATIONS
Anti-Reverse Cap Analog (ARCA)
3′-O-Me-m7G(5′) ppp(5′)G
Produces 100% translatable capped transcripts
Co-transcriptional capping with T7 (NEB #M0251), SP6 (NEB #M0207) and T3 RNA polymerases
Synthesis of m7G capped RNA for in vitro splicing assays
Synthesis of m7G capped RNA for transfection or microinjection
Standard Cap Analog
m7G(5′)ppp(5′)G
Co-transcriptional capping with T7, SP6 and T3 RNA polymerases
Synthesis of m7G capped RNA for in vitro splicing assays
Synthesis of m7G capped RNA for transfection or microinjection
Unmethylated Cap Analog
G (5′)ppp(5′)G
Co-transcriptional capping with T7, SP6 and T3 RNA polymerases
Synthesis of unmethylated G capped RNA
Methylated Cap Analog for A+1 sites
m7G(5′)ppp(5′)A
Co-transcriptional capping with T7 RNA polymerase from the phi2.5 promoter that contains an A at the transcription initiation site
Synthesis of m7G capped RNA for in vitro splicing assays
Synthesis of m7G capped RNA for transfection or microinjection
Unmethylated Cap Analog for A+1 sites
G(5′)ppp(5′)A
Co-transcriptional capping with T7 RNA polymerase from the phi2.5 promoter that contains an A at the transcription initiation site
Synthesis of unmethylated G capped RNA
Synthesis of A capped RNA