OneTaq Buffer Recommendations

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  • NEB recommends choosing your OneTaq buffer based on amplicon % GC content.

    AMPLICON %
    GC CONTENT
    RECOMMENDED
    DEFAULT BUFFER
    OPTIMIZATION NOTES
    <50% GC OneTaq Standard Reaction Buffer* Adjust annealing temperature, primer/
    template concentration, etc., if needed.
    50–65% GC OneTaq Standard Reaction Buffer* OneTaq GC Reaction Buffer can be
    used to enhance performance of difficult
    amplicons.
    >65% GC OneTaq GC Reaction Buffer* OneTaq GC Reaction Buffer with
    10–20% OneTaq High GC Enhancer
    can be used to enhance performance of
    difficult amplicons.
    * Mg-free formulations are also available.