Competent Cell Selection Guide

Product NEB # Characteristics
Strains for Cloning and Subcloning
NEB® Stable Competent E. coli (High Efficiency) C3040I
C3040H
  • High quality plasmid preparations due to endA mutation
  • Rapid growth recA strain
  • T1 phage resistance (fhuA)
  • Value pricing
  • Free of animal products
NEB® Turbo Competent E. coli (High Efficiency) C2984I
C2984H
  • Fastest growth – colonies visible after 6.5 hours
  • Plasmid preparation after 4 hours
  • Tight control/expression of toxic genes (F´lacIq)
NEB® 5-alpha Competent E. coli (High Efficiency) C2987U
C2987R
C2987P
C2987I
C2987H
  • Versatile cloning strain
  • DH5α™ derivative
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency) C2992I
C2992H
  • Toxic gene cloning
  • F´ strain with extremely high transformation efficiency
NEB® 10-beta Competent E. coli (High Efficiency) C3019I
C3019H
  • Large plasmid and BAC cloning
  • DH10B™ derivative
dam-/dcm- Competent E. coli C2925I
C2925H
  • Dam/Dcm methyltransferase free plasmid growth
Strains for Protein Expression
NEB® Express Competent E. coli (High Efficiency) C2523I
C2523H
  • Versatile non-T7 expression strain
  • Protease deficient
NEB® Express Iq Competent E. coli (High Efficiency) C3037I
  • Control of IPTG induced expression from Plac, Ptac,Ptrc and T5lac
  • Protease deficient
T7 Express Competent E. coli (High Efficiency) C2566I
C2566H
  • Most popular T7 expression strain
  • Protease deficient
T7 Express lysY Competent E. coli (High Efficiency) C3010AA
C3010A
  • T7 expression
  • Protease deficient
  • Better reduction of basal expression
T7 Express lysY/Iq Competent E. coli (High Efficiency) C3013I
C3013AA
C3013A
  • T7 expression
  • Protease deficient
  • Highest level of expression control
SHuffle® Express Competent E. coli C3028J
  • Protease deficient/B strain
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
SHuffle® T7 Express Competent E. coli C3029J
  • T7 expression
  • Protease deficient/B strain
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
SHuffle® T7 Express lysY Competent E. coli C3030J
  • T7 expression
  • Protease deficient/B strain
  • Tightly controlled expression of toxic proteins
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
SHuffle® T7 Competent E. coli C3026J
  • T7 expression/K12 strain
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
BL21 Competent E. coli C2530H
  • Routine non-T7 expression
BL21(DE3) Competent E. coli C2527I
C2527H
  • Routine T7 expression
Lemo21(DE3) Competent E. coli C2528J
  • Tunable T7 expression for difficult targets
NiCo21(DE3) Competent E. coli C2529H
  • Improved purity of target proteins isolated by IMAC

There are many properties to consider when choosing a strain for your experiments. Requirements such as plasmid preparation, blue-white screening, cytoplasmic disulfide bond formation and fast colony growth necessitate specific strain choices. Please check the Strain Properties Chart for a particular experiment.

DH5α™ and DH10β™ are trademarks of Invitrogen.
SHuffle® is a registered trademark of New England Biolabs, Inc.

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