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Competent Cell Selection Guide

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  • ProductNEB #Characteristics
    Strains for Cloning and Subcloning
    NEB® Stable Competent E. coli (High Efficiency)C3040H
    C3040I
    • High quality plasmid preparations due to endA mutation
    • Rapid growth recA strain
    • T1 phage resistance (fhuA)
    • Value pricing
    • Free of animal products
    NEB® Turbo Competent E. coli (High Efficiency)C2984H
    C2984I
    • Fastest growth – colonies visible after 6.5 hours
    • Plasmid preparation after 4 hours
    • Tight control/expression of toxic genes (F´lacI8)
    NEB® 5-alpha Competent E. coli (High Efficiency)C2987H
    C2987I
    C2987P
    C2987R
    C2987U
    • Versatile cloning strain
    • DH5α™ derivative
    NEB® 5-alpha F' Iq Competent E. coli (High Efficiency)C2992H
    C2992I
    • Toxic gene cloning
    • F´ strain with extremely high transformation efficiency
    NEB® 10-beta Competent E. coli (High Efficiency)C3019H
    C3019I
    • Large plasmid and BAC cloning
    • DH10B™ derivative
    dam-/dcm- Competent E. coliC2925H
    C2925I
    • Dam/Dcm methyltransferase free plasmid growth
    Strains for Protein Expression
    NEB® Express Competent E. coli (High Efficiency)C2523H
    C2523I
    • Versatile non-T7 expression strain
    • Protease deficient
    NEB® Express Iq Competent E. coli (High Efficiency)C3037I
    • Control of IPTG induced expression from Plac, Ptac,Ptrc and T5lac
    • Protease deficient
    T7 Express Competent E. coli (High Efficiency)C2566H
    C2566I
    C2566A
    C2566AA
    • Most popular T7 expression strain
    • Protease deficient
    T7 Express lysY Competent E. coli (High Efficiency)C3010I
    C3010A
    C3010AA
    • T7 expression
    • Protease deficient
    • Better reduction of basal expression
    T7 Express lysY/Iq Competent E. coli (High Efficiency)C3013I
    C3013A
    C3013AA
    • T7 expression
    • Protease deficient
    • Highest level of expression control
    T7 Express Crystal Competent E. coli (High Efficiency)C3022I
    • T7 expression
    • Protease deficient
    • SeMet labeling for protein crystallography
    SHuffle® Express Competent E. coliC3028H
    • Protease deficient/B strain
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    SHuffle® T7 Express Competent E. coliC3029H
    • T7 expression
    • Protease deficient/B strain
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    SHuffle® T7 Express lysY Competent E. coliC3030H
    • T7 expression
    • Protease deficient/B strain
    • Tightly controlled expression of toxic proteins
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    SHuffle® T7 Competent E. coliC3026H
    • T7 expression/K12 strain
    • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
    BL21 Competent E. coliC2530H
    • Routine non-T7 expression
    BL21(DE3) Competent E. coliC2527H
    C2527I
    • Routine T7 expression
    Lemo21(DE3) Competent E. coliC2528H
    • Tunable T7 expression for difficult targets
    NiCo21(DE3) Competent E. coliC2529H
    • Improved purity of target proteins isolated by IMAC

    There are many properties to consider when choosing a strain for your experiments. Requirements such as plasmid preparation, blue-white screening, cytoplasmic disulfide bond formation and fast colony growth necessitate specific strain choices. Please check the Strain Properties Chart for a particular experiment.

    DH5α™ and DH10β™ are trademarks of Invitrogen.
    SHuffle® is a registered trademark of New England Biolabs, Inc.