Enzyme Finder - Sequence > 6 Bases

Enzyme

Sequence

Cut Site

Overhang

Properties (NEB Enzymes Only)

CCTCAGC

C

C

T

C

A

G

C

G

G

A

G

T

/

C

G

nick

CCTCAGC

C

C

/

T

C

A

G

C

G

G

A

G

T

/

C

G

5´ - TCA

CCTCAGC

C

C

/

T

C

A

G

C

G

G

A

G

T

C

G

nick

GGCGCGCC

G

G

/

C

G

C

G

C

C

C

C

G

C

G

C

/

G

G

5´ - CGCG

GGCGCGCC

G

G

/

C

G

C

G

C

C

C

C

G

C

G

C

/

G

G

5´ - CGCG

GCGATCGC

G

C

G

A

T

/

C

G

C

C

G

C

/

T

A

G

C

G

AT - 3´

GGCCGGCC

G

G

C

C

G

G

/

C

C

C

C

/

G

G

C

C

G

G

CCGG - 3´

GCGGCCGC

G

C

/

G

G

C

C

G

C

C

G

C

C

G

G

/

C

G

5´ - GGCC

NotI-HF™ RE-Mix®

GCGGCCGC

G

C

/

G

G

C

C

G

C

C

G

C

C

G

G

/

C

G

5´ - GGCC

GCGGCCGC

G

C

G

G

C

C

G

C

C

G

C

C

G

G

C

G

5´ - GGCC

TTAATTAA

T

T

A

A

T

/

T

A

A

A

A

T

/

T

A

A

T

T

AT - 3´

TTAATTAA

T

T

A

A

T

/

T

A

A

A

A

T

/

T

A

A

T

T

AT - 3´

GTTTAAAC

G

T

T

T

/

A

A

A

C

C

A

A

A

/

T

T

T

G

blunt

CCTGCAGG

C

C

T

G

C

A

/

G

G

G

G

/

A

C

G

T

C

C

TGCA - 3´

CCTGCAGG

C

C

T

G

C

A

G

G

G

G

A

C

G

T

C

C

TGCA - 3´

CRCCGGYG

C

R

/

C

C

G

G

Y

G

G

Y

G

G

C

C

/

R

C

5´ - CCGG

ATTTAAAT

A

T

T

T

/

A

A

A

T

T

A

A

A

/

T

T

T

A

blunt

GCTCTTC

G

C

T

C

T

T

C

N

/

C

G

A

G

A

A

G

N

nick

GCTCTTC

G

C

T

C

T

T

C

C

G

A

G

A

A

G

5´ - NNN

GCTCTTC

G

C

T

C

T

T

C

C

G

A

G

A

A

G

5´ - NNN

GGCCNNNNNGGCC

G

G

C

C

N

N

N

N

/

N

G

G

C

C

C

C

G

G

N

/

N

N

N

N

C

C

G

G

NNN - 3´

CAANNNNNGTGG

C

A

A

N

N

N

N

N

G

T

G

G

G

T

T

N

N

N

N

N

C

A

C

C

NN - 3´

AbsI

CCTCGAGG

C

C

/

T

C

G

A

G

G

G

G

A

G

C

T

/

C

C

5´ - TCGA

CciNI

GCGGCCGC

G

C

/

G

G

C

C

G

C

C

G

C

C

G

G

/

C

G

5´ - GGCC

FspAI

RTGCGCAY

R

T

/

G

C

/

G

C

A

Y

Y

A

C

G

/

C

/

G

T

R

blunt

MauBI

CGCGCGCG

C

G

/

C

G

C

G

C

G

G

C

G

C

G

C

/

G

C

5´ - CGCG

MreI

CGCCGGCG

C

G

/

C

C

G

G

C

G

G

C

G

G

C

C

/

G

C

5´ - CCGG

MssI

GTTTAAAC

G

T

T

T

/

A

A

A

C

C

A

A

A

/

T

T

T

G

blunt

PalAI

GGCGCGCC

G

G

/

C

G

/

C

G

C

C

C

C

G

C

/

G

C

/

G

G

5´ - CGCG

RgaI

GCGATCGC

G

C

G

A

T

/

C

G

C

C

G

C

/

T

A

G

C

G

AT - 3´

RigI

GGCCGGCC

G

G

C

C

G

/

G

/

C

C

C

C

/

G

G

C

C

G

G

CCGG - 3´

SdaI

CCTGCAGG

C

C

T

G

C

A

/

G

G

G

G

/

A

C

G

T

C

C

TGCA - 3´

SfaAI

GCGATCGC

G

C

G

A

T

/

C

G

C

C

G

C

/

T

A

G

C

G

AT - 3´

SgfI

GCGATCGC

G

C

G

A

T

/

C

G

C

C

G

C

/

T

A

G

C

G

AT - 3´

SgrDI

CGTCGACG

C

G

T

C

G

A

C

G

N

N

N

N

G

C

A

G

C

T

G

C

5´ - TCGA

SgsI

GGCGCGCC

G

G

/

C

G

C

G

C

C

C

C

G

C

G

C

/

G

G

5´ - CGCG

SmiI

ATTTAAAT

A

T

T

T

/

A

A

A

T

T

A

A

A

/

T

T

T

A

blunt

SrfI

GCCCGGGC

G

C

C

C

/

G

G

G

C

C

G

G

G

/

C

C

C

G

blunt

Sse232I

CGCCGGCG

C

G

/

C

C

G

G

C

G

G

C

G

G

C

C

/

G

C

5´ - CCGG

Sse8387I

CCTGCAGG

C

C

/

T

G

C

A

/

G

G

G

G

/

A

C

G

/

T

C

C

TGCA - 3´

LguI

GCTCTTC

G

C

T

C

T

T

C

C

G

A

G

A

A

G

5´ - NNN

PciSI

GCTCTTC

G

C

T

C

T

T

C

N

/

N

N

N

C

G

A

G

A

A

G

N

N

N

N

/

5´ - NNN

AarI

CACCTGC

C

A

C

C

T

G

C

G

T

G

G

A

C

G

5´ - NNNN

AjuI

GAANNNNNNNTTGG

G

A

A

N

N

N

N

N

N

N

T

T

G

G

C

T

T

N

N

N

N

N

N

N

A

A

C

C

NNNNN - 3´

AloI

GAACNNNNNNTCC

G

A

A

C

N

N

N

N

N

N

T

C

C

C

T

T

G

N

N

N

N

N

N

A

G

G

NNNNN - 3´

BarI

GAAGNNNNNNTAC

G

A

A

G

N

N

N

N

N

N

T

A

C

C

T

T

C

N

N

N

N

N

N

A

T

G

NNNNN - 3´

PpiI

GAACNNNNNCTC

G

A

A

C

N

N

N

N

N

C

T

C

C

T

T

G

N

N

N

N

N

G

A

G

NNNNN - 3´

PsrI

GAACNNNNNNTAC

G

A

A

C

N

N

N

N

N

N

T

A

C

C

T

T

G

N

N

N

N

N

N

A

T

G

NNNNN - 3´