Enzyme Finder - Sequence > 6 Bases

Enzyme Sequence Cut Site Overhang Properties (NEB Enzymes Only)
Nb.BbvCI CCTCAGC
C C T C A G C
G G A G T / C G
nick     
BbvCI CCTCAGC
C C / T C A G C
G G A G T / C G
5´ - TCA      
Nt.BbvCI CCTCAGC
C C / T C A G C
G G A G T C G
nick
    
AscI GGCGCGCC
G G / C G C G C C
C C G C G C / G G
5´ - CGCG       
AsiSI GCGATCGC
G C G A T / C G C
C G C / T A G C G

AT - 3´       
FseI GGCCGGCC
G G C C G G / C C
C C / G G C C G G
CCGG - 3´        
NotI GCGGCCGC
G C / G G C C G C
C G C C G G / C G
5´ - GGCC       
NotI-HF™ GCGGCCGC
G C G G C C G C
C G C C G G C G
5´ - GGCC       
PacI TTAATTAA
T T A A T / T A A
A A T / T A A T T
AT - 3´        
PmeI GTTTAAAC
G T T T / A A A C
C A A A / T T T G
blunt        
SbfI CCTGCAGG
C C T G C A / G G
G G / A C G T C C
TGCA - 3´      
SbfI-HF™ CCTGCAGG
C C T G C A G G
G G A C G T C C
TGCA - 3´      
SgrAI CRCCGGYG
C R / C C G G Y G
G Y G G C C / R C
5´ - CCGG     
SwaI ATTTAAAT
A T T T / A A A T
T A A A / T T T A
blunt      
Nt.BspQI GCTCTTC
G C T C T T C N /
C G A G A A G N
nick      
BspQI GCTCTTC
G C T C T T C
C G A G A A G
5´ - NNN      
SapI GCTCTTC
G C T C T T C
C G A G A A G
5´ - NNN     
SfiI GGCCNNNNNGGCC
G G C C N N N N / N G G C C
C C G G N / N N N N C C G G
NNN - 3´        
CspCI CAANNNNNGTGG
C A A N N N N N G T G G
G T T N N N N N C A C C
NN - 3´       
AbsI CCTCGAGG
C C / T C G A G G
G G A G C T / C C
5´ - TCGA
CciNI GCGGCCGC
G C / G G C C G C
C G C C G G / C G
5´ - GGCC
FspAI RTGCGCAY
R T / G C / G C A Y
Y A C G / C / G T R
blunt
MauBI CGCGCGCG
C G / C G C G C G
G C G C G C / G C
5´ - CGCG
MreI CGCCGGCG
C G / C C G G C G
G C G G C C / G C
5´ - CCGG
MssI GTTTAAAC
G T T T / A A A C
C A A A / T T T G
blunt
PalAI GGCGCGCC
G G / C G / C G C C
C C G C / G C / G G
5´ - CGCG
RgaI GCGATCGC
G C G A T / C G C
C G C / T A G C G
AT - 3´
RigI GGCCGGCC
G G C C G / G / C C
C C / G G C C G G
CCGG - 3´
SdaI CCTGCAGG
C C T G C A / G G
G G / A C G T C C
TGCA - 3´
SfaAI GCGATCGC
G C G A T / C G C
C G C / T A G C G
AT - 3´
SgfI GCGATCGC
G C G A T / C G C
C G C / T A G C G
AT - 3´
SgrDI CGTCGACG
C G T C G A C G N N
N N G C A G C T G C
5´ - TCGA
SgsI GGCGCGCC
G G / C G C G C C
C C G C G C / G G
5´ - CGCG
SmiI ATTTAAAT
A T T T / A A A T
T A A A / T T T A
blunt
SrfI GCCCGGGC
G C C C / G G G C
C G G G / C C C G
blunt
Sse232I CGCCGGCG
C G / C C G G C G
G C G G C C / G C
5´ - CCGG
Sse8387I CCTGCAGG
C C / T G C A / G G
G G / A C G / T C C
TGCA - 3´
LguI GCTCTTC
G C T C T T C
C G A G A A G
5´ - NNN
PciSI GCTCTTC
G C T C T T C N / N N N
C G A G A A G N N N N /
5´ - NNN
AarI CACCTGC
C A C C T G C
G T G G A C G
5´ - NNNN
AjuI GAANNNNNNNTTGG
G A A N N N N N N N T T G G
C T T N N N N N N N A A C C
NNNNN - 3´
AloI GAACNNNNNNTCC
G A A C N N N N N N T C C
C T T G N N N N N N A G G
NNNNN - 3´
BarI GAAGNNNNNNTAC
G A A G N N N N N N T A C
C T T C N N N N N N A T G
NNNNN - 3´
PpiI GAACNNNNNCTC
G A A C N N N N N C T C
C T T G N N N N N G A G
NNNNN - 3´
PsrI GAACNNNNNNTAC
G A A C N N N N N N T A C
C T T G N N N N N N A T G
NNNNN - 3´