Cloning Plasmids and DNAs

Cloning Plasmid/DNA NEB # Features Concentration MW/Size Size Price
LITMUS 28i Vector N3528S
  • Multipurpose cloning plasmids
  • Unique MCS includes many restriction enzymes with 4-base overhangs
  • T7 promoter
  • Amp resistance
  • Blue/white selection
500 µg/ml 1.8 x 106 Da/
2,823 bp
20 µg
$88
pBR322 Vector N3033S/L
  • Commonly used cloning vectors
  • Tet, Amp resistance
1,000 µg/ml
2.83 x 106 DA/
4,361 bp
50/250 µg
$65/$260
pUC19 Vector N3041S/L 1,000 µg/ml
1.75 x 106 Da/
2,686 bp
50/250 µg
$65/$260
M13mp18 RF I DNA N4018S
  • Phage vectors derived from bacteriophage M13
  • DNA, covalently closed circular
  • 13 Unique RE sites with β-gal gene
  • Blue-white selection
100 µg/ml
7,249 bp 10 µg
$74
M13mp18 Single-stranded DNA N4040S 250 µg/ml
7,249 bases 10 µg
$33
Lambda DNA N3011S/L
  • Commonly used DNA substrate
500 µg/ml
31.5 x 106 Da/
48,502 bp
250/1,250 µg
$62/$248
Lambda DNA (N6-methyladenine-free) N3013S/L 500 µg/ml
31.5 x 106 Da/
48,502 bp
250/1,250 µg
$62/$248
ΦX174 RF I DNA
N3021S/L
  • Commonly used DNA substrate
  • Covalently closed circular form of X174
1,000 µg/ml
3.5 x 106 Da/
5,386 bp
30/150 µg
$65/$260
ΦX174 Virion DNA
N3023S/L
  • Single-stranded viral DNA
1,000 µg/ml
1.7 x 106 Da/
5,386 bases
50/250 µg
$71/$284