cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA Synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.

cDNA SYNTHESIS	NEB #	FEATURES	SIZE
KIT
ProtoScript® II First Strand cDNA Synthesis Kit	E6560S E6560L	Generates cDNA at least 10 kb in length Contains ProtoScript II Reverse Transcriptase, an enzyme with increased thermostability and reduced RNase H activity Convenient 2-tube kit Includes dNTPs, Oligo-dT primer and Random Primer Mix	30 reactions 150 reactions
ProtoScript® First Strand cDNA Synthesis Kit	E6300S E6300L	Generates cDNA at least 5 kb in length Contains M-MuLV Reverse Transcriptase Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix	30 reactions 150 reactions
AMV First Strand cDNA Synthesis Kit	E6550S	Generates cDNA at least 10 kb in length Enables cDNA synthesis from difficult templates that require higher reaction temperatures Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix	30 reactions
STANDALONE REAGENT
ProtoScript® II Reverse Transcriptase An alternative to SuperScript® II	M0368S M0368L M0368X	RNase H- mutant of M-MuLV Reverse Transcriptase with increased thermostability and reduced RNase H activity Increased reaction temperatures (37-50°C)	4,000 units 10,000 units 40,000 units
M-MuLV Reverse Transcriptase	M0253S M0253L	Robust reverse transcriptase for a variety of templates Standard reaction temperatures (37-45°C)	10,000 units 50,000 units
AMV Reverse Transcriptase	M0277S M0277L M0277T	Robust reverse transcriptase for a broad temperature range (37-52°C) Can be used for templates requiring higher reaction temperatures	200 units 1,000 units 500 units

ProtoScript® is a registered trademark of New England Biolabs, Inc.