cDNA Synthesis Selection Chart

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  • NEB offers several reagents for cDNA Synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.

    cDNA SYNTHESIS NEB # FEATURES SIZE
    KIT
    ProtoScript® II First Strand cDNA Synthesis Kit E6560S
    E6560L
    • Generates cDNA at least 10 kb in length
    • Contains ProtoScript II Reverse Transcriptase, an enzyme with increased thermostability and reduced RNase H activity
    • Convenient 2-tube kit Includes dNTPs, Oligo-dT primer and Random Primer Mix
    30 reactions
    150 reactions
    ProtoScript® First Strand cDNA Synthesis Kit E6300S
    E6300L
    • Generates cDNA at least 5 kb in length
    • Contains M-MuLV Reverse Transcriptase
    • Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix
    30 reactions
    150 reactions
    AMV First Strand cDNA Synthesis Kit E6550S
    • Generates cDNA at least 10 kb in length
    • Enables cDNA synthesis from difficult templates that require higher reaction temperatures
    • Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix
    30 reactions
    STANDALONE REAGENT
    ProtoScript® II Reverse Transcriptase
    An alternative to SuperScript® II
    M0368S
    M0368L
    M0368X
    • RNase H- mutant of M-MuLV Reverse Transcriptase with increased thermostability and reduced RNase H activity
    • Increased reaction temperatures (37-50°C)
    4,000 units
    10,000 units
    40,000 units
    M-MuLV Reverse Transcriptase M0253S
    M0253L
    • Robust reverse transcriptase for a variety of templates
    • Standard reaction temperatures (37-45°C)
    10,000 units
    50,000 units
    AMV Reverse Transcriptase M0277S
    M0277L
    M0277T
    • Robust reverse transcriptase for a broad temperature range (37-52°C)
    • Can be used for templates requiring higher reaction temperatures
    200 units
    1,000 units
    500 units

    ProtoScript® is a registered trademark of New England Biolabs, Inc.