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cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA Synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.

cDNA SYNTHESIS NEB # FEATURES SIZE
KIT
LunaScript® RT SuperMix Kit E3010S
E3010L
  • Ideal for cDNA synthesis in a two-step RT-qPCR workflow
  • Single-tube supermix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna Reverse Transcriptase
  • Visible blue tracking dye for easy reaction setup
  • Fast 13-minute protocol
 25 reactions
100 reactions
LunaScript RT Master Mix Kit (Primer Free)
 E3025S
E3025L
  • Ideal for first strand cDNA synthesis
  • Compatible with random primers, oligo dT primers, and gene-specific primers for maximum cDNA synthesis flexibility
  • 5X master mix contains dNTPs, Murine RNase Inhibitor and Luna Reverse Transcriptase
  • Visible blue tracking dye for easy reaction setup
  • Fast 13-minute protocol 
 25 reactions
100 reactions
ProtoScript® II First Strand cDNA Synthesis Kit E6560S
E6560L
  • Generates cDNA at least 10 kb in length
  • Contains ProtoScript II Reverse Transcriptase, an enzyme with increased thermostability and reduced RNase H activity
  • Convenient 2-tube kit
  • Includes dNTPs, Oligo-dT primer and Random Primer Mix
 30 reactions
150 reactions
ProtoScript First Strand cDNA Synthesis Kit E6300S
E6300L
  • Generates cDNA at least 5 kb in length
  • Contains M-MuLV Reverse Transcriptase
  • Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix
 30 reactions
150 reactions
Template Switching RT Enzyme Mix
 M0466S
M0466L:
  • Incorporates a universal adaptor sequence at the 3´ end of cDNA during the RT reaction
  • Enzyme mix and buffer are optimized for efficient template switching
  • RT enzyme mix includes RNase inhibitor
  • High sensitivity for cDNA amplification – enables transcriptome analysis by RNA-seq from single cells or as low as 2 pg of human total RNA
  • Robust and simple workflow for 5´ Rapid Amplication of cDNA Ends (RACE)
  • Retains the complete 5´ end of transcripts for 2nd strand cDNA synthesis 
 20 reactions
100 reactions
STANDALONE REAGENT
ProtoScript II Reverse Transcriptase
An alternative to SuperScript® II		 M0368S
M0368L
M0368X
  • RNase H- mutant of M-MuLV Reverse Transcriptase with increased thermostability and reduced RNase H activity
  • Increased reaction temperatures (37-50°C)
 4,000 units
10,000 units
40,000 units
M-MuLV Reverse Transcriptase M0253S
M0253L
  • Robust reverse transcriptase for a variety of templates
  • Standard reaction temperatures (37-45°C)
 10,000 units
50,000 units
AMV Reverse Transcriptase M0277S
M0277L
  • Robust reverse transcriptase for a broad temperature range (37-52°C)
  • Can be used for templates requiring higher reaction temperatures
 200 units
1,000 units
WarmStart® RTx Reverse Transcriptase M0380S
M0380L
  • Permits room temperature reaction set-up
  • Increased reaction temperatures (50–65°C)
  • Optimized for RT-LAMP isothermal detection
50 reactions
250 reactions

LunaScript, ProtoScript, and WarmStart are registered trademarks of New England Biolabs, Inc. SuperScript is a trademark of Thermo Fisher Scientific, Inc.