cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA Synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.

cDNA SYNTHESIS	NEB #	FEATURES	SIZE
KIT
ProtoScript® II First Strand cDNA Synthesis Kit	E6560S E6560L	Generates cDNA at least 10 kb in length Contains ProtoScript II Reverse Transcriptase, an enzyme with increased thermostability and reduced RNase H activity Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix	30 reactions 150 reactions
ProtoScript® First Strand cDNA Synthesis Kit	E6300S E6300L	Generates cDNA at least 5 kb in length Contains M-MuLV Reverse Transcriptase Convenient 2-tube kit includes dNTPs, Oligo-dT primer and Random Primer Mix	30 reactions 150 reactions
STANDALONE REAGENT
ProtoScript® II Reverse Transcriptase An alternative to SuperScript® II	M0368S M0368L M0368X	RNase H- mutant of M-MuLV Reverse Transcriptase with increased thermostability and reduced RNase H activity Increased reaction temperatures (42-48°C)	4,000 units 10,000 units 40,000 units
M-MuLV Reverse Transcriptase	M0253S M0253L	Robust reverse transcriptase for a variety of templates Standard reaction temperatures (37-42°C)	10,000 units 50,000 units
AMV Reverse Transcriptase	M0277S M0277L M0277T	Robust reverse transcriptase for a broad temperature range (37-52°C) Can be used for templates requiring higher reaction temperatures	200 units 1,000 units 500 units
WarmStart RTx Reverse Transcriptase	M0380S M0380L	Permits room temperature reaction set-up Increased reaction temperatures (50–65°C) Optimized for RT-LAMP isothermal detection	50 reactions 250 reactions

ProtoScript^® is a registered trademark of New England Biolabs, Inc.