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Buffer and Diluent Formulation Table

The following chart lists the components of various common buffers and diluents from New England Biolabs at 1X concentration.

Components	T4 Polynucleotide Kinase Reaction Buffer \| B0201	T4 DNA Ligase Reaction Buffer \| B0202	Antarctic Phosphatase Reaction Buffer \| B0289	Exonuclease I Reaction Buffer \| B0293	Exonuclease VII Reaction Buffer \| B0379	StickTogether™ DNA Ligase Buffer \| B0535	NEBuffer™ 1.1 \| B7201	NEBuffer™ 2.1 \| B7202	NEBuffer™ 3.1 \| B7203	CutSmart® Buffer \| B7204	Diluent A \| B8001	Diluent B \| B8002	Diluent C \| B8003	ThermoPol^® Reaction Buffer Pack \| B9004
Tris-HCl	70 mM	50 mM			50 mM	66 mM		10 mM	50 mM		10 mM	10 mM	10 mM	20 mM
Bis-Tris-Propane-HCl			50 mM				10 mM
Tris-acetate										20 mM
Sodium Phosphate
Potassium Acetate										50 mM
Magnesium Acetate										10 mM
EDTA					8 mM						0.1 mM	0.1 mM	0.1 mM
NaCl								50 mM	100 mM			300 mM	250 mM
KCl											50 mM			10 mM
MgCl₂	10 mM	10 mM	1 mM	6.7 mM		10 mM	10 mM	10 mM	10 mM
ZnCl₂			0.1 mM
MgSO₄														2 mM
(NH₄)₂SO₄														10 mM
Glycine-KOH				67 mM
BSA							100 µg/ml	100 µg/ml	100 µg/ml	100 µg/ml	200 µg/ml	500 µg/ml	200 µg/ml
ATP		1 mM				1 mM
DTT	5 mM	10 mM				1 mM					1 mM	1 mM	1 mM
β-ME				10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000)						7.5%
Triton® X-100													0.15%	0.1%
Glycerol											50%	50%	50%
pH @ 25	7.60	7.50	6.00	9.50	8.00	7.60	7.00	7.90	7.90	7.90	7.40	7.40	7.40	8.80