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Buffer and Diluent Formulation Table

The following chart lists the components of various common buffers and diluents from New England Biolabs at 1X concentration.

Components	NEBuffer™ 1.1 \| B7201	NEBuffer™ 2.1 \| B7202	NEBuffer™ 3.1 \| B7203	CutSmart® Buffer \| B7204	T4 DNA Ligase Reaction Buffer \| B0202	NEBNext® Quick Ligation Reaction Buffer \| B6058	ThermoPol^®Reaction Buffer Pack \| B9004	T4 Polynucleotide Kinase Reaction Buffer \| B0201	Antarctic Phosphatase Reaction Buffer \| B0289	Exonuclease I Reaction Buffer \| B0293	Exonuclease VII Reaction Buffer \| B0379	Diluent A \| B8001	Diluent B \| B8002	Diluent C \| B8003
Tris-HCl		10 mM	50 mM		50 mM	66 mM	20 mM	70 mM			50 mM	10 mM	10 mM	10 mM
Bis-Tris-Propane-HCl	10 mM								50 mM
Tris-acetate				20 mM
Sodium Phosphate
Potassium Acetate				50 mM
Magnesium Acetate				10 mM
EDTA											8 mM	0.1 mM	0.1 mM	0.1 mM
NaCl		50 mM	100 mM										300 mM	250 mM
KCl							10 mM					50 mM
MgCl₂	10 mM	10 mM	10 mM		10 mM	10 mM		10 mM	1 mM	6.7 mM
ZnCl₂									0.1 mM
MgSO₄							2 mM
(NH₄)₂SO₄							10 mM
Glycine-KOH										67 mM
BSA	100 μg/ml	100 μg/ml	100 μg/ml	100 μg/ml								200 μg/ml	500 μg/ml	200 μg/ml
ATP					1 mM	1 mM
DTT					10 mM	1 mM		5 mM				1 mM	1 mM	1 mM
β-ME										10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000)						6%
Triton® X-100							0.1%							0.15%
Glycerol												50%	50%	50%
pH @ 25	7	7.9	7.9	7.9	7.5	7.6	8.8	7.6	6	9.5	8	7.4	7.4	7.4

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