NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

Buffer and Diluent Formulation Table

Components NEBuffer™ 1.1 | B7201 NEBuffer™ 2.1 | B7202 NEBuffer™ 3.1 | B7203 CutSmart® Buffer | B7204 T4 DNA Ligase Reaction Buffer | B0202 NEBNext® Quick Ligation Reaction Buffer | B6058 ThermoPol® Reaction Buffer Pack | B9004 T4 Polynucleotide Kinase Reaction Buffer | B0201 NEBuffer™ EcoRI | B0101 Antarctic Phosphatase Reaction Buffer | B0289 Exonuclease I Reaction Buffer | B0293 Exonuclease VII Reaction Buffer | B0379 Diluent A | B8001 Diluent B | B8002 Diluent C | B8003
Tris-HCl 10 mM 50 mM 50 mM 66 mM 20 mM 70 mM 100 mM 50 mM 10 mM 10 mM 10 mM
Bis-Tris-Propane-HCl 10 mM 50 mM
Tris-acetate 20 mM
Sodium Phosphate 50 mM
Potassium Acetate 50 mM
Magnesium Acetate 10 mM
EDTA 8 mM 0.1 mM 0.1 mM 0.1 mM
NaCl 50 mM 100 mM 50 mM 300 mM 250 mM
KCl 10 mM 50 mM
MgCl2 10 mM 10 mM 10 mM 10 mM 10 mM 10 mM 10 mM 1 mM 6.7 mM
ZnCl2 0.1 mM
MgSO4 2 mM
(NH4)2SO4 10 mM
Glycine-KOH 67 mM
BSA 100 μg/ml 100 μg/ml 100 μg/ml 100 μg/ml 200 μg/ml 500 μg/ml 200 μg/ml
ATP 1 mM 1 mM
DTT 10 mM 1 mM 5 mM 1 mM 1 mM 1 mM
β-ME 10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000) 6%
Triton® X-100 0.1% 0.025% 0.15%
Glycerol 50% 50% 50%
pH @ 25 7 7.9 7.9 7.9 7.5 7.6 8.8 7.6 7.5 6 9.5 8 7.4 7.4 7.4
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