Buffer and Diluent Formulation Table

The following chart lists the components of various common buffers and diluents from New England Biolabs at 1x concentration.

Components	NEBuffer 1.1 B7201S	NEBuffer 2.1 B7202S	NEBuffer 3.1 B7203S	CutSmart Buffer B7204S	T4 DNA Ligase Reaction Buffer B0202S	Quick Ligation Reaction Buffer B6058S	Thermopol Reaction Buffer Pack B9004S	T4 Polynucleotide Kinase Buffer B0201S	NEBuffer EcoRI B0101S	Antarctic Phosphatase Reaction Buffer B0289S	Exonuclease I Reaction Buffer B0293S	Exonuclease VII Reaction Buffer	Diluent A \| B8001S	Diluent B B8002S	Diluent C B8003S
Tris-HCl		10mM	50mM		50mM	66mM	20mM	70mM	100mM			50 mM	10mM	10mM	10mM
Bis-Tris-Propane-HCl	10mM									50mM
Tris-acetate				20mM
Sodium Phosphate												50 mM
Potassium Acetate				50mM
Magnesium Acetate				10mM
EDTA												8 mM	0.1mM	0.1mM	0.1mM
NaCl		50mM	100mM						50mM					300mM	250mM
KCl							10mM						50mM
MgCl2	10mM	10mM	10mM		10mM	10mM		10mM	10mM	1mM	6.7mM
ZnCl2										0.1mM
MgSO4							2mM
(NH4)2SO4							10mM
Glycine-KOH											67mM
BSA	100μg/ml	100μg/ml	100μg/ml	100μg/ml									200μg/ml	500μg/ml	200μg/ml
ATP					1mM	1mM
DTT					10mM	1mM		5mM					1mM	1mM	1mM
β-ME											10mM
2-mercapto-ethanol												10 mM
Polyethylene glycol (PEG 6000)						6%
Triton® X-100							0.10%		0.025%						0.15%
Glycerol													50%	50%	50%
pH @ 25	7.0	7.9	7.9	7.9	7.5	7.6	8.8	7.6	7.5	6.0	9.5	8	7.4	7.4	7.4