Buffer and Diluent Formulation Table
Components | NEBuffer™ 1.1 | B7201 | NEBuffer™ 2.1 | B7202 | NEBuffer™ 3.1 | B7203 | CutSmart® Buffer | B7204 | T4 DNA Ligase Reaction Buffer | B0202 | NEBNext® Quick Ligation Reaction Buffer | B6058 | ThermoPol® Reaction Buffer Pack | B9004 | T4 Polynucleotide Kinase Reaction Buffer | B0201 | Antarctic Phosphatase Reaction Buffer | B0289 | Exonuclease I Reaction Buffer | B0293 | Exonuclease VII Reaction Buffer | B0379 | Diluent A | B8001 | Diluent B | B8002 | Diluent C | B8003 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tris-HCl | 10 mM | 50 mM | 50 mM | 66 mM | 20 mM | 70 mM | 50 mM | 10 mM | 10 mM | 10 mM | ||||
Bis-Tris-Propane-HCl | 10 mM | 50 mM | ||||||||||||
Tris-acetate | 20 mM | |||||||||||||
Sodium Phosphate | ||||||||||||||
Potassium Acetate | 50 mM | |||||||||||||
Magnesium Acetate | 10 mM | |||||||||||||
EDTA | 8 mM | 0.1 mM | 0.1 mM | 0.1 mM | ||||||||||
NaCl | 50 mM | 100 mM | 300 mM | 250 mM | ||||||||||
KCl | 10 mM | 50 mM | ||||||||||||
MgCl2 | 10 mM | 10 mM | 10 mM | 10 mM | 10 mM | 10 mM | 1 mM | 6.7 mM | ||||||
ZnCl2 | 0.1 mM | |||||||||||||
MgSO4 | 2 mM | |||||||||||||
(NH4)2SO4 | 10 mM | |||||||||||||
Glycine-KOH | 67 mM | |||||||||||||
BSA | 100 μg/ml | 100 μg/ml | 100 μg/ml | 100 μg/ml | 200 μg/ml | 500 μg/ml | 200 μg/ml | |||||||
ATP | 1 mM | 1 mM | ||||||||||||
DTT | 10 mM | 1 mM | 5 mM | 1 mM | 1 mM | 1 mM | ||||||||
β-ME | 10 mM | |||||||||||||
2-mercapto-ethanol | ||||||||||||||
Polyethylene glycol (PEG 6000) | 6% | |||||||||||||
Triton® X-100 | 0.1% | 0.15% | ||||||||||||
Glycerol | 50% | 50% | 50% | |||||||||||
pH @ 25 | 7 | 7.9 | 7.9 | 7.9 | 7.5 | 7.6 | 8.8 | 7.6 | 6 | 9.5 | 8 | 7.4 | 7.4 | 7.4 |