Applications of Polymerases

NEB offers a number of Polymerases for PCR. For other applications, see the selection chart below.

	Bst DNA L.Frag	Taq	Vent_R®	Vent_R® (exo-)	Deep Vent_R™	Deep Vent_R™ (exo-)	9°N_m™	Ther- minator™	T7 DNA	E.coli Poly I	Klenow Frag	Klenow exo-	Reverse Transc- riptase	T4 DNA	T7 RNA	SP6	Phusion®*	OneTaq®
Labeling in vitro
3' fill-in	(+)	+	(+)	+	(+)	+	(+)		(+)	(+)	(+)	+		(+)			+	(+)
3' exchange			+		+				+	(+)	+			+			+
nick-translation		+								+								(+)
replacement synthesis			(+)		(+)				(+)	(+)	(+)			+			+
random priming	+	+	+	+	+	+	+				+	+					+	+
RNA probes															+	+
Cloning
creating blunt ends
5' overhang fill-in			(+)		(+)		(+)		(+)	(+)	+			+			+
3' overhang removal			+		+		(+)		(+)	(+)	+			+			+	(+)
nested deletions														+
Sequencing
DNA	(+)	(+)		(+)		(+)		+		(+)	+	(+)	(+)
RNA													+
Mutagenesis
oligonucleotide directed			(+)		(+)				+		(+)			+			+
misincorporation		+									+
Mapping
transcript													+
cDNA
synthesis
1st strand													+
2nd strand	(+)		(+)		(+)					(+)	+
cloning
Okayama-Berg													+
In vitro transcription															+	+

+ = recommended enzyme for this application
(+) = usable, but not the enzyme of choice

Vent_R® and OneTaq® are registered trademarks of New England Biolabs, Inc.
DeepVent_R™, 9°N_m™ and Therminator™ are trademarks of New England Biolabs, Inc.

* Notice to Customers:
Phusion^® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.

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