Applications of Polymerases
NEB offers a number of Polymerases for PCR. For other applications, see the selection chart below.
| Bst DNA L.Frag |
Taq | VentR® | VentR® (exo-) |
Deep VentR™ |
Deep VentR™ (exo-) |
9°Nm™ | Ther- minator™ |
T7 DNA |
E.coli Poly I |
Klenow Frag |
Klenow exo- |
Reverse Transc- riptase |
T4 DNA |
T7 RNA |
SP6 |
Phusion®* | OneTaq® | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Labeling in vitro | ||||||||||||||||||
| 3' fill-in | (+) | + | (+) | + | (+) | + | (+) | (+) | (+) | (+) | + | (+) | + | (+) | ||||
| 3' exchange | + | + | + | (+) | + | + | + | |||||||||||
| nick-translation | + | + | (+) | |||||||||||||||
| replacement synthesis | (+) | (+) | (+) | (+) | (+) | + | + | |||||||||||
| random priming | + | + | + | + | + | + | + | + | + | + | + | |||||||
| RNA probes | + | + | ||||||||||||||||
| Cloning | ||||||||||||||||||
| creating blunt ends | ||||||||||||||||||
| 5' overhang fill-in | (+) | (+) | (+) | (+) | (+) | + | + | + | ||||||||||
| 3' overhang removal | + | + | (+) | (+) | (+) | + | + | + | (+) | |||||||||
| nested deletions | + | |||||||||||||||||
| Sequencing | ||||||||||||||||||
| DNA | (+) | (+) | (+) | (+) | + | (+) | + | (+) | (+) | |||||||||
| RNA | + | |||||||||||||||||
| Mutagenesis | ||||||||||||||||||
| oligonucleotide directed | (+) | (+) | + | (+) | + | + | ||||||||||||
| misincorporation | + | + | ||||||||||||||||
| Mapping | ||||||||||||||||||
| transcript | + | |||||||||||||||||
| cDNA | ||||||||||||||||||
| synthesis | ||||||||||||||||||
| 1st strand | + | |||||||||||||||||
| 2nd strand | (+) | (+) | (+) | (+) | + | |||||||||||||
| cloning | ||||||||||||||||||
| Okayama-Berg | + | |||||||||||||||||
| In vitro transcription | + | + | ||||||||||||||||
+ = recommended enzyme for this application
(+) = usable, but not the enzyme of choice
VentR® and OneTaq® are registered trademarks of New England Biolabs, Inc.
DeepVentR™, 9°Nm™ and Therminator™ are trademarks of New England Biolabs, Inc.
* Notice to Customers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
