New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

Applications of Polymerases

NEB offers a number of Polymerases for PCR. For other applications,  see the selection chart below.

  Bst
DNA
L.Frag
Taq Vent® Vent®
(exo-)
Deep
Vent®
Deep
Vent®
(exo-)
9°Nm Ther-
minator™
T7
DNA
E.coli
Poly I
Klenow
Frag
Klenow
exo-
Reverse
Transc-
riptase
T4
DNA
T7
RNA
SP6
Phusion®* OneTaq®
Labeling in vitro
3' fill-in  (+)  + (+) + (+) + (+)   (+) (+) (+) +   (+)     + (+)
3' exchange     +   +       + (+) +     +     +  
nick-translation   +               +               (+)
replacement synthesis     (+)   (+)       (+) (+) (+)     +     +  
random priming + + + + + + +       + +         + +
RNA probes                             + +    
Cloning
creating blunt ends
5' overhang fill-in     (+)   (+)   (+)   (+) (+) +     +     +  
3' overhang removal     +   +   (+)   (+) (+) +     +     + (+)
nested deletions                           +        
Sequencing
DNA (+) (+)   (+)    (+)   +   (+) + (+) (+)          
RNA                         +          
Mutagenesis
oligonucleotide directed     (+)   (+)       +   (+)     +     +  
misincorporation   +                 +              
Mapping
transcript                         +          
cDNA
synthesis
1st strand                         +          
2nd strand (+)   (+)   (+)         (+) +              
cloning
Okayama-Berg                         +          
In vitro transcription                             + +    

+ = recommended enzyme for this application
(+) = usable, but not the enzyme of choice

Vent® and OneTaq® are registered trademarks of New England Biolabs, Inc.
DeepVent®, 9°Nm™ and Therminator™ are trademarks of New England Biolabs, Inc.

* Notice to Customers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.

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