NEB will be closed Thursday, November 23, 2017 and Friday, November 24, 2017. Orders placed will process Monday, November 27, 2017 for delivery on Tuesday, November 28,2017.

Applications of Polymerases

NEB offers a number of Polymerases for PCR. For other applications,  see the selection chart below.

  Bst
DNA
L.Frag
Taq VentR® VentR®
(exo-)
Deep
VentR
Deep
VentR
(exo-)
9°Nm Ther-
minator™
T7
DNA
E.coli
Poly I
Klenow
Frag
Klenow
exo-
Reverse
Transc-
riptase
T4
DNA
T7
RNA
SP6
Phusion®* OneTaq®
Labeling in vitro
3' fill-in  (+)  + (+) + (+) + (+)   (+) (+) (+) +   (+)     + (+)
3' exchange     +   +       + (+) +     +     +  
nick-translation   +               +               (+)
replacement synthesis     (+)   (+)       (+) (+) (+)     +     +  
random priming + + + + + + +       + +         + +
RNA probes                             + +    
Cloning
creating blunt ends
5' overhang fill-in     (+)   (+)   (+)   (+) (+) +     +     +  
3' overhang removal     +   +   (+)   (+) (+) +     +     + (+)
nested deletions                           +        
Sequencing
DNA (+) (+)   (+)    (+)   +   (+) + (+) (+)          
RNA                         +          
Mutagenesis
oligonucleotide directed     (+)   (+)       +   (+)     +     +  
misincorporation   +                 +              
Mapping
transcript                         +          
cDNA
synthesis
1st strand                         +          
2nd strand (+)   (+)   (+)         (+) +              
cloning
Okayama-Berg                         +          
In vitro transcription                             + +    

+ = recommended enzyme for this application
(+) = usable, but not the enzyme of choice

VentR® and OneTaq® are registered trademarks of New England Biolabs, Inc.
DeepVentR™, 9°Nm™ and Therminator™ are trademarks of New England Biolabs, Inc.

* Notice to Customers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.

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