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Applications of Polymerases

NEB offers a number of Polymerases for PCR. For other applications,  see the selection chart below.

  Bst
DNA
L.Frag		 Taq VentR® VentR®
(exo-)		 Deep
VentR™		 Deep
VentR™
(exo-)		 9°Nm™ Ther-
minator™		 T7
DNA 		E.coli
Poly I		 Klenow
Frag		 Klenow
exo-		 Reverse
Transc-
riptase		 T4
DNA		 T7
RNA		 SP6
 Phusion®* OneTaq®
Labeling in vitro
3' fill-in  (+)  + (+) + (+) + (+)   (+) (+) (+) +   (+)     + (+)
3' exchange     +   +       + (+) +     +     +  
nick-translation   +               +               (+)
replacement synthesis     (+)   (+)       (+) (+) (+)     +     +  
random priming + + + + + + +       + +         + +
RNA probes                             + +    
Cloning
creating blunt ends
5' overhang fill-in     (+)   (+)   (+)   (+) (+) +     +     +  
3' overhang removal     +   +   (+)   (+) (+) +     +     + (+)
nested deletions                           +        
Sequencing
DNA (+) (+)   (+)    (+)   +   (+) + (+) (+)          
RNA                         +          
Mutagenesis
oligonucleotide directed     (+)   (+)       +   (+)     +     +  
misincorporation   +                 +              
Mapping
transcript                         +          
cDNA
synthesis
1st strand                         +          
2nd strand (+)   (+)   (+)         (+) +              
cloning
Okayama-Berg                         +          
In vitro transcription                             + +    

+ = recommended enzyme for this application
(+) = usable, but not the enzyme of choice

VentR® and OneTaq® are registered trademarks of New England Biolabs, Inc.
DeepVentR™, 9°Nm™ and Therminator™ are trademarks of New England Biolabs, Inc.

* Notice to Customers:
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.

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