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    Applications of Polymerases

    NEB offers a number of Polymerases for PCR. For other applications,  see the selection chart below.

      Bst
    DNA
    L.Frag    		 Taq VentR® VentR®
    (exo-)    		 Deep
    VentR™    		 Deep
    VentR™
    (exo-)    		 9°Nm™ Ther-
    minator™    		 T7
    DNA         		E.coli
    Poly I    		 Klenow
    Frag    		 Klenow
    exo-    		 Reverse
    Transc-
    riptase    		 T4
    DNA    		 T7
    RNA    		 SP6
    		 Phusion®* OneTaq®
    Labeling in vitro
    3' fill-in  (+)  + (+) + (+) + (+)   (+) (+) (+) +   (+)     + (+)
    3' exchange     +   +       + (+) +     +     +  
    nick-translation   +               +               (+)
    replacement synthesis     (+)   (+)       (+) (+) (+)     +     +  
    random priming + + + + + + +       + +         + +
    RNA probes                             + +    
    Cloning
    creating blunt ends
    5' overhang fill-in     (+)   (+)   (+)   (+) (+) +     +     +  
    3' overhang removal     +   +   (+)   (+) (+) +     +     + (+)
    nested deletions                           +        
    Sequencing
    DNA (+) (+)   (+)    (+)   +   (+) + (+) (+)          
    RNA                         +          
    Mutagenesis
    oligonucleotide directed     (+)   (+)       +   (+)     +     +  
    misincorporation   +                 +              
    Mapping
    transcript                         +          
    cDNA
    synthesis
    1st strand                         +          
    2nd strand (+)   (+)   (+)         (+) +              
    cloning
    Okayama-Berg                         +          
    In vitro transcription                             + +    

    + = recommended enzyme for this application
    (+) = usable, but not the enzyme of choice

    VentR® and OneTaq® are registered trademarks of New England Biolabs, Inc.
    DeepVentR™, 9°Nm™ and Therminator™ are trademarks of New England Biolabs, Inc.

    * Notice to Customers:
    Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.