New England Biolabs
  • My NEB
  • Print
  • PDF
  • PCR Selection Tool

    For over 35 years, New England Biolabs, Inc. has been a world leader in the discovery and production of reagents for the life science industry. NEB offers a wide range of DNA polymerases and, through our commitment to research, ensures the development of innovative, high-quality tools for PCR.

    When choosing a polymerase for PCR, we recommend starting with OneTaq® (NEB #M0480) or Q5® DNA Polymerase (NEB #M0491) for routine PCR applications, and NEBNext® High-Fidelity Master Mix (NEB #M0541) for next gen sequencing (NGS) library construction (shown below in orange). These all offer robust amplification, and can be used on a wide range of templates (routine, AT- and GC-rich). Q5 and NEBNext Master Mix (a Q5 formulation) provide the added benefit of maximum fidelity.

    ★ indicates recommended choice for application

    STANDARD PCR HIGH-FIDELITY PCR SPECIALTY PCR
    OneTaq®/
    OneTaq Hot Start
    Taq/
    Hot Start Taq
    Highest Fidelity
    Moderate
    Fidelity
    Long
    Amplicons
    Bisulfite
    Sequencing
    Blood Direct
    PCR
    Q5®/Q5 Hot Start
    Phusion®(1)/
    Phusion(1) Flex
    VentR®/Deep VentR
    LongAmp®/
    LongAmp Hot Start Taq
    EpiMark® Hot Start Taq
    TaqHemo Klen
    PROPERTIES
    Fidelity vs. Taq 2X 1X >100X >50X 5-6X 2X 1X ND
    Amplicon Size <6 kb ≤5 kb ≤20 kb ≤20 kb ≤6 kb ≤30 kb ≤1 kb ≤2 kb
    Extension Time 1 kb/min 1 kb/min 6 kb/min 4 kb/min 1 kb/min 1.2 kb/min 1 kb/min 0.5 kb/min
    Resulting Ends 3' A/Blunt 3' A Blunt Blunt Blunt 3' A/Blunt 3' A 3' A
    3'→5' exo Yes No Yes Yes Yes Yes No No
    5'→3' exo Yes Yes No No No Yes Yes No
    Units/50µl Reaction 1.25 1.25 1.0 1.0 0.5-1.0 5.0 1.25 N/A
    Annealing Temperature Tm- Tm-  Tm+3  Tm+3 Tm- Tm- Tm- Tm-
    APPLICATIONS
    Routine PCR
    Colony PCR
           ● 
    Enhanced Fidelity

     
    High Fidelity
    High Yield
    Fast  
    Long Amplicon
    GC-rich Targets
    AT-rich Targets
    High Throughput
    Multiplex PCR (2)
    Extraction-free PCR
    DNA-labeling
    Site-directed Mutagenesis
    Bisulfite Sequencing
    NGS APPLICATIONS
    NGS Library Amplification (3)
    FORMATS
    Hot Start Available
    Kit
    Master Mix Available
    Direct Gel Loading Available (4)   (5)

    (1) Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
    (2) Use Multiplex PCR 5X Master Mix.
    (3) Use NEBNext High-Fidelity 2X PCR Master Mix.
    (4) Use Crimson Taq DNA Polymerase.
    (5) Use Crimson LongAmp Taq DNA Polymerase.

    Hemo KlenTaq™ is a trademark of DNA Polymerase Technology, Inc.
    OneTaq®, Q5®, NEBNext®, VentR® and EpiMark® are registered trademarks of New England Biolabs, Inc.
    Deep VentR™ is a trademark of New England Biolabs, Inc.
    Phusion® is a registered trademark of Thermo Fisher Scientific.