New England Biolabs: Reagents for the Life Sciences Industry

Enzyme Finder

Use this tool to select restriction enzymes by name, sequence, overhang or type.  Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. In search results, enzymes supplied by NEB are listed first with their NEB icons, and displayed as links to their product page.


EnzymeSequenceCut SiteOverhangProperties
FspEICC
CCNNNNNNNNNNNN/NNNN
GGNNNNNNNNNNNNNNNN/
5′ - NNNN recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp 
Nt.CviPIICCD
/CCD
GGH
nick cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
AciICCGC
C/CGC
GGC/G
5′ - CG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
AluIAGCT
AG/CT
TC/GA
blunt timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BfaICTAG
C/TAG
GAT/C
5′ - TA recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BfuCIGATC
/GATC
CTAG/
5′ - GATC timesaver 5min cpg heat inactivation yes icon_CutSmart incubation temp
BstUICGCG
CG/CG
GC/GC
blunt timesaver 5min cpg heat inactivation no icon_CutSmart incubation temp
CviAIICATG
C/ATG
GTA/C
5′ - AT timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart 25 degree incubation temp
CviKI-1RGCY
RG/CY
YC/GR
blunt recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
CviQIGTAC
G/TAC
CAT/G
5′ - TA timesaver 5min recombinant cloned at neb heat inactivation no icon_neb3_1 25 degree incubation temp
DpnIGATC
GA/TC
CT/AG
blunt timesaver 5min cpg recombinant heat inactivation yes icon_CutSmart incubation temp
DpnIIGATC
/GATC
CTAG/
5′ - GATC timesaver 5min dam recombinant heat inactivation yes unique buffer incubation temp
FatICATG
/CATG
GTAC/
5′ - CATG recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp
HaeIIIGGCC
GG/CC
CC/GG
blunt timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HhaIGCGC
GCG/C
C/GCG
CG- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HinP1IGCGC
G/CGC
CGC/G
5′ - CG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HpaIICCGG
C/CGG
GGC/C
5′ - CG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HpyCH4IVACGT
A/CGT
TGC/A
5′ - CG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HpyCH4VTGCA
TG/CA
AC/GT
blunt timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
LpnPICCDG
CCDGNNNNNNNNNN/NNNN
GGHCNNNNNNNNNNNNNN/
5′ - NNNN recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp 
MboIGATC
/GATC
CTAG/
5′ - GATC timesaver 5min dam cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
MluCIAATT
/AATT
TTAA/
5′ - AATT timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
MnlICCTC
CCTCNNNNNNN/
GGAGNNNNNN/N
N- 3′ timesaver 5min recombinant heat inactivation yes icon_CutSmart incubation temp
MseITTAA
T/TAA
AAT/T
5′ - TA timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
MspICCGG
C/CGG
GGC/C
5′ - CG timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
MspJICNNR
CNNRNNNNNNNNN/NNNN
GNNYNNNNNNNNNNNNN/
5′ - NNNN recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp 
NlaIIICATG
CATG/
/GTAC
CATG- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PhoIGGCC
GG/CC
CC/GG
blunt dcm cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
RsaIGTAC
GT/AC
CA/TG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
Sau3AIGATC
/GATC
CTAG/
5′ - GATC cpg recombinant cloned at neb heat inactivation yes icon_neb1_1 incubation temp
TaqαITCGA
T/CGA
AGC/T
5′ - CG timesaver 5min dam recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Tsp509IAATT
/AATT
TTAA/
5′ - AATT heat inactivation no NEBuffer 1 incubation temp
AlwIGGATC
GGATCNNNN/N
CCTAGNNNNN/
5′ - N dam recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
ApeKIGCWGC
G/CWGC
CGWC/G
5′ - CWG timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
AvaIIGGWCC
G/GWCC
CCWG/G
5′ - GWC timesaver 5min dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BbvIGCAGC
GCAGCNNNNNNNN/NNNN
CGTCGNNNNNNNNNNNN/
5′ - NNNN timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BccICCATC
CCATCNNNN/N
GGTAGNNNNN/
5′ - N recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BceAIACGGC
ACGGCNNNNNNNNNNNN/NN
TGCCGNNNNNNNNNNNNNN/
5′ - NN cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BcoDIGTCTC
GTCTCN/NNNN
CAGAGNNNNN/
5′ - NNNN timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BsmAIGTCTC
GTCTCN/NNNN
CAGAGNNNNN/
5′ - NNNN timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BsmFIGGGAC
GGGACNNNNNNNNNN/NNNN
CCCTGNNNNNNNNNNNNNN/
5′ - NNNN dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BspCNICTCAG
CTCAGNNNNNNNNN/
GAGTCNNNNNNN/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart 25 degree incubation temp  sam
BsrIACTGG
ACTGGN/
TGAC/CN
GN- 3′ timesaver 5min heat inactivation yes icon_neb3_1 incubation temp
BssKICCNGG
/CCNGG
GGNCC/
5′ - CCNGG timesaver 5min dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BstNICCWGG
CC/WGG
GGW/CC
5′ - W timesaver 5min recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
BtsCIGGATG
GGATGNN/
CCTAC/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BtsIMutICAGTG
CAGTGNN/
GTCAC/NN
NN- 3′ recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
DdeICTNAG
C/TNAG
GANT/C
5′ - TNA timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
FauICCCGC
CCCGCNNNN/NN
GGGCGNNNNNN/
5′ - NN cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Fnu4HIGCNGC
GC/NGC
CGN/CG
5′ - N timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
FokIGGATG
GGATGNNNNNNNNN/NNNN
CCTACNNNNNNNNNNNNN/
5′ - NNNN dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HgaIGACGC
GACGCNNNNN/NNNNN
CTGCGNNNNNNNNNN/
5′ - NNNNN cpg recombinant cloned at neb heat inactivation yes icon_neb1_1 incubation temp
HinfIGANTC
G/ANTC
CTNA/G
5′ - ANT timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HphIGGTGA
GGTGANNNNNNNN/
CCACTNNNNNNN/N
N- 3′ timesaver 5min dam dcm recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Hpy188ITCNGA
TCN/GA
AG/NCT
N- 3′ dam recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Hpy99ICGWCG
CGWCG/
/GCWGC
CGWCG- 3′ cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HpyAVCCTTC
CCTTCNNNNNN/
GGAAGNNNNN/N
N- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HpyCH4IIIACNGT
ACN/GT
TG/NCA
N- 3′ recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
MboIIGAAGA
GAAGANNNNNNNN/
CTTCTNNNNNNN/N
N- 3′ dam recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
MlyIGAGTC
GAGTCNNNNN/
CTCAGNNNNN/
blunt timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
NciICCSGG
CC/SGG
GGS/CC
5′ - S timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
Nt.AlwIGGATC
GGATCNNNN/
CCTAGNNNN
nick dam recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Nt.BsmAIGTCTC
GTCTCN/
CAGAGN
nick cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Nt.BstNBIGAGTC
GAGTCNNNN/
CTCAGNNNN
nick recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
PleIGAGTC
GAGTCNNNN/N
CTCAGNNNNN/
5′ - N cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PspGICCWGG
/CCWGG
GGWCC/
5′ - CCWGG dcm recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
Sau96IGGNCC
G/GNCC
CCNG/G
5′ - GNC dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
ScrFICCNGG
CC/NGG
GGN/CC
5′ - N dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
SfaNIGCATC
GCATCNNNNN/NNNN
CGTAGNNNNNNNNN/
5′ - NNNN cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
StyD4ICCNGG
/CCNGG
GGNCC/
5′ - CCNGG timesaver 5min dcm cpg recombinant heat inactivation yes icon_CutSmart incubation temp
TfiIGAWTC
G/AWTC
CTWA/G
5′ - AWT timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
TseIGCWGC
G/CWGC
CGWC/G
5′ - CWG timesaver 5min cpg heat inactivation no icon_CutSmart incubation temp
Tsp45IGTSAC
/GTSAC
CASTG/
5′ - GTSAC icon_CutSmart incubation temp
TspRICASTG
NNCASTGNN/
/NNGTSACNN
NNCASTGNN- 3′ timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
AatIIGACGTC
GACGT/C
C/TGCAG
ACGT- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
Acc65IGGTACC
G/GTACC
CCATG/G
5′ - GTAC timesaver 5min dcm cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
AccIGTMKAC
GT/MKAC
CAKM/TG
5′ - MK timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
AclIAACGTT
AA/CGTT
TTGC/AA
5′ - CG timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
AcuICTGAAG
CTGAAGNNNNNNNNNNNNNNNN/
GACTTCNNNNNNNNNNNNNN/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp  sam
AfeIAGCGCT
AGC/GCT
TCG/CGA
blunt cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
AflIICTTAAG
C/TTAAG
GAATT/C
5′ - TTAA timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
AflIIIACRYGT
A/CRYGT
TGYRC/A
5′ - CRYG recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
AgeIACCGGT
A/CCGGT
TGGCC/A
5′ - CCGG cpg recombinant cloned at neb heat inactivation yes icon_neb1_1 incubation temp blue white
AgeI-HF®ACCGGT
A/CCGGT
TGGCC/A
5′ - CCGG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
AgeI-HF® RE-Mix®ACCGGT
A/CCGGT
TGGCC/A
5′ - CCGG timesaver 5min cpg recombinant cloned at neb heat inactivation yes incubation temp engineered
ApaIGGGCCC
GGGCC/C
C/CCGGG
GGCC- 3′ timesaver 5min dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart 25 degree incubation temp
ApaLIGTGCAC
G/TGCAC
CACGT/G
5′ - TGCA timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
ApoIRAATTY
R/AATTY
YTTAA/R
5′ - AATT timesaver 5min star activity recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
ApoI-HFRAATTY
R/AATTY
YTTAA/R
5′ - AATT timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
AseIATTAAT
AT/TAAT
TAAT/TA
5′ - TA timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
AvaICYCGRG
C/YCGRG
GRGCY/C
5′ - YCGR timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
AvrIICCTAGG
C/CTAGG
GGATC/C
5′ - CTAG timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp blue white
BaeGIGKGCMC
GKGCM/C
C/MCGKG
KGCM- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BamHIGGATCC
G/GATCC
CCTAG/G
5′ - GATC timesaver 5min recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp blue white
BamHI-HF®GGATCC
G/GATCC
CCTAG/G
5′ - GATC timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered blue white
BanIGGYRCC
G/GYRCC
CCRYG/G
5′ - GYRC dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BanIIGRGCYC
GRGCY/C
C/YCGRG
RGCY- 3′ recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BbsIGAAGAC
GAAGACNN/NNNN
CTTCTGNNNNNN/
5′ - NNNN timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp
BbsI-HF®GAAGAC
GAAGACNN/NNNN
CTTCTGNNNNNN/
5′ - NNNN timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
BciVIGTATCC
GTATCCNNNNNN/
CATAGGNNNNN/N
N- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BclITGATCA
T/GATCA
ACTAG/T
5′ - GATC timesaver 5min dam recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
BclI-HFTGATCA
T/GATCA
ACTAG/T
5′ - GATC timesaver 5min dam recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
BfuAIACCTGC
ACCTGCNNNN/NNNN
TGGACGNNNNNNNN/
5′ - NNNN timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BglIIAGATCT
A/GATCT
TCTAG/A
5′ - GATC timesaver 5min recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp blue white
BmgBICACGTC
CAC/GTC
GTG/CAG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BmrIACTGGG
ACTGGGNNNNN/
TGACCCNNNN/N
N- 3′ recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp
BmtIGCTAGC
GCTAG/C
C/GATCG
CTAG- 3′ recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BmtI-HF®GCTAGC
GCTAG/C
C/GATCG
CTAG- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
BpmICTGGAG
CTGGAGNNNNNNNNNNNNNNNN/
GACCTCNNNNNNNNNNNNNN/NN
NN- 3′ recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BpuEICTTGAG
CTTGAGNNNNNNNNNNNNNNNN/
GAACTCNNNNNNNNNNNNNN/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp  sam
BsaAIYACGTR
YAC/GTR
RTG/CAY
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BsaHIGRCGYC
GR/CGYC
CYGC/RG
5′ - CG timesaver 5min dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BsaIGGTCTC
GGTCTCN/NNNN
CCAGAGNNNNN/
5′ - NNNN dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BsaI-HF®GGTCTC
GGTCTCN/NNNN
CCAGAGNNNNN/
5′ - NNNN timesaver 5min dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
BsaJICCNNGG
C/CNNGG
GGNNC/C
5′ - CNNG recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BsaWIWCCGGW
W/CCGGW
WGGCC/W
5′ - CCGG timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BseRIGAGGAG
GAGGAGNNNNNNNNNN/
CTCCTCNNNNNNNN/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BseYICCCAGC
C/CCAGC
GGGTC/G
5′ - CCAG cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BsgIGTGCAG
GTGCAGNNNNNNNNNNNNNNNN/
CACGTCNNNNNNNNNNNNNN/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp  sam
BsiEICGRYCG
CGRY/CG
GC/YRGC
RY- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BsiHKAIGWGCWC
GWGCW/C
C/WCGWG
WGCW- 3′ recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BsiWICGTACG
C/GTACG
GCATG/C
5′ - GTAC timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BsiWI-HF®CGTACG
C/GTACG
GCATG/C
5′ - GTAC timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
BsmBICGTCTC
CGTCTCN/NNNN
GCAGAGNNNNN/
5′ - NNNN timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BsmIGAATGC
GAATGCN/
CTTAC/GN
CN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BsoBICYCGRG
C/YCGRG
GRGCY/C
5′ - YCGR timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Bsp1286IGDGCHC
GDGCH/C
C/HCGDG
DGCH- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BspDIATCGAT
AT/CGAT
TAGC/TA
5′ - CG dam cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BspEITCCGGA
T/CCGGA
AGGCC/T
5′ - CCGG timesaver 5min dam cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
BspHITCATGA
T/CATGA
AGTAC/T
5′ - CATG timesaver 5min dam recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BspMIACCTGC
ACCTGCNNNN/NNNN
TGGACGNNNNNNNN/
5′ - NNNN recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BsrBICCGCTC
CCG/CTC
GGC/GAG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BsrDIGCAATG
GCAATGNN/
CGTTAC/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp
BsrFαIRCCGGY
R/CCGGY
YGGCC/R
5′ - CCGG timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
BsrFIRCCGGY
R/CCGGY
YGGCC/R
5′ - CCGG cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BsrGITGTACA
T/GTACA
ACATG/T
5′ - GTAC timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp
BsrGI-HF®TGTACA
T/GTACA
ACATG/T
5′ - GTAC timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
BssHIIGCGCGC
G/CGCGC
CGCGC/G
5′ - CGCG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
BssSαICACGAG
C/ACGAG
GTGCT/C
5′ - ACGA timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
BssSICACGAG
C/ACGAG
GTGCT/C
5′ - ACGA recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
BstBITTCGAA
TT/CGAA
AAGC/TT
5′ - CG timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BstYIRGATCY
R/GATCY
YCTAG/R
5′ - GATC timesaver 5min recombinant cloned at neb heat inactivation no icon_neb2_1 incubation temp
BstZ17IGTATAC
GTA/TAC
CAT/ATG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
BstZ17I-HF®GTATAC
GTATAC
CATATG
No Sequence Supplied timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
BtgICCRYGG
C/CRYGG
GGYRC/C
5′ - CRYG timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BtgZIGCGATG
GCGATGNNNNNNNNNN/NNNN
CGCTACNNNNNNNNNNNNNN/
5′ - NNNN cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
BtsαIGCAGTG
GCAGTGNN/
CGTCAC/NN
NN- 3′ timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
BtsIGCAGTG
GCAGTGNN/
CGTCAC/NN
NN- 3′ recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Cac8IGCNNGC
GCN/NGC
CGN/NCG
blunt timesaver 5min cpg heat inactivation yes icon_CutSmart incubation temp
ClaIATCGAT
AT/CGAT
TAGC/TA
5′ - CG timesaver 5min dam cpg recombinant heat inactivation yes icon_CutSmart incubation temp
DraITTTAAA
TTT/AAA
AAA/TTT
blunt timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
EaeIYGGCCR
Y/GGCCR
RCCGG/Y
5′ - GGCC dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
EagICGGCCG
C/GGCCG
GCCGG/C
5′ - GGCC timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
EagI-HF®CGGCCG
C/GGCCG
GCCGG/C
5′ - GGCC timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
EarICTCTTC
CTCTTCN/NNN
GAGAAGNNNN/
5′ - NNN timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
EciIGGCGGA
GGCGGANNNNNNNNNNN/
CCGCCTNNNNNNNNN/NN
NN- 3′ cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Eco53kIGAGCTC
GAG/CTC
CTC/GAG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
EcoP15ICAGCAG
CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN/NN
GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN/
5′ - NN timesaver 5min recombinant heat inactivation yes icon_neb3_1 incubation temp 
EcoRIGAATTC
G/AATTC
CTTAA/G
5′ - AATT timesaver 5min cpg recombinant cloned at neb heat inactivation yes unique buffer incubation temp blue white
EcoRI-HF®GAATTC
G/AATTC
CTTAA/G
5′ - AATT timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
EcoRI-HF® RE-Mix®GAATTC
G/AATTC
CTTAA/G
5′ - AATT timesaver 5min cpg recombinant cloned at neb heat inactivation yes incubation temp engineered
EcoRVGATATC
GAT/ATC
CTA/TAG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
EcoRV-HF®GATATC
GAT/ATC
CTA/TAG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
EcoRV-HF® RE-Mix®GATATC
GAT/ATC
CTA/TAG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes incubation temp engineered
FspITGCGCA
TGC/GCA
ACG/CGT
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
HaeIIRGCGCY
RGCGC/Y
Y/CGCGR
GCGC- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
HincIIGTYRAC
GTY/RAC
CAR/YTG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
HindIIIAAGCTT
A/AGCTT
TTCGA/A
5′ - AGCT recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp blue white
HindIII-HF®AAGCTT
A/AGCTT
TTCGA/A
5′ - AGCT timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
HpaIGTTAAC
GTT/AAC
CAA/TTG
blunt cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
Hpy166IIGTNNAC
GTN/NAC
CAN/NTG
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Hpy188IIITCNNGA
TC/NNGA
AGNN/CT
5′ - NN dam cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
KasIGGCGCC
G/GCGCC
CCGCG/G
5′ - GCGC cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
KpnIGGTACC
GGTAC/C
C/CATGG
GTAC- 3′ recombinant heat inactivation no icon_neb1_1 incubation temp blue white
KpnI-HF®GGTACC
GGTAC/C
C/CATGG
GTAC- 3′ timesaver 5min recombinant heat inactivation no icon_CutSmart incubation temp engineered blue white
KpnI-HF® RE-Mix®GGTACC
GGTAC/C
C/CATGG
GTAC- 3′ timesaver 5min recombinant cloned at neb heat inactivation no incubation temp engineered
MfeICAATTG
C/AATTG
GTTAA/C
5′ - AATT recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp blue white
MfeI-HF®CAATTG
C/AATTG
GTTAA/C
5′ - AATT timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered blue white
MfeI-HF® RE-Mix®CAATTG
C/AATTG
GTTAA/C
5′ - AATT timesaver 5min recombinant cloned at neb heat inactivation no incubation temp engineered
MluIACGCGT
A/CGCGT
TGCGC/A
5′ - CGCG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
MluI-HF®ACGCGT
A/CGCGT
TGCGC/A
5′ - CGCG timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
MmeITCCRAC
TCCRACNNNNNNNNNNNNNNNNNNNN/
AGGYTGNNNNNNNNNNNNNNNNNN/NN
NN- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp  sam
MscITGGCCA
TGG/CCA
ACC/GGT
blunt dcm recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
MspA1ICMGCKG
CMG/CKG
GKC/GMC
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
NaeIGCCGGC
GCC/GGC
CGG/CCG
blunt cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
NarIGGCGCC
GG/CGCC
CCGC/GG
5′ - CG cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Nb.BsmIGAATGC
GAATGC
CTTAC/G
nick recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
Nb.BsrDIGCAATG
GCAATGN
CGTTAC/N
nick recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
Nb.BssSICACGAG
CACGAG
GTGCT/C
nick recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
Nb.BtsIGCAGTG
GCAGTGN
CGTCAC/N
nick recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
NcoICCATGG
C/CATGG
GGTAC/C
5′ - CATG timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
NcoI-HF®CCATGG
C/CATGG
GGTAC/C
5′ - CATG timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
NcoI-HF® RE-Mix®CCATGG
C/CATGG
GGTAC/C
5′ - CATG timesaver 5min recombinant cloned at neb heat inactivation yes incubation temp engineered
NdeICATATG
CA/TATG
GTAT/AC
5′ - TA timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
NgoMIVGCCGGC
G/CCGGC
CGGCC/G
5′ - CCGG timesaver 5min cpg recombinant heat inactivation no icon_CutSmart incubation temp
NheIGCTAGC
G/CTAGC
CGATC/G
5′ - CTAG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp blue white
NheI-HF®GCTAGC
G/CTAGC
CGATC/G
5′ - CTAG timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
NheI-HF® RE-Mix®GCTAGC
G/CTAGC
CGATC/G
5′ - CTAG timesaver 5min cpg recombinant cloned at neb heat inactivation yes incubation temp engineered
NlaIVGGNNCC
GGN/NCC
CCN/NGG
blunt dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
NmeAIIIGCCGAG
GCCGAGNNNNNNNNNNNNNNNNNNNNN/
CGGCTCNNNNNNNNNNNNNNNNNNN/NN
NN- 3′ recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp  sam
NruITCGCGA
TCG/CGA
AGC/GCT
blunt timesaver 5min dam cpg recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
NruI-HF®TCGCGA
TCG/CGA
AGC/GCT
blunt timesaver 5min dam cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
NsiIATGCAT
ATGCA/T
T/ACGTA
TGCA- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
NsiI-HF®ATGCAT
ATGCA/T
T/ACGTA
TGCA- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
NspIRCATGY
RCATG/Y
Y/GTACR
CATG- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PaeR7ICTCGAG
C/TCGAG
GAGCT/C
5′ - TCGA timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
PciIACATGT
A/CATGT
TGTAC/A
5′ - CATG recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp
PluTIGGCGCC
GGCGC/C
C/CGCGG
GCGC- 3′ cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PmlICACGTG
CAC/GTG
GTG/CAC
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PsiITTATAA
TTA/TAA
AAT/ATT
blunt recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PspOMIGGGCCC
G/GGCCC
CCCGG/G
5′ - GGCC dcm cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
PstICTGCAG
CTGCA/G
G/ACGTC
TGCA- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
PstI-HF®CTGCAG
CTGCA/G
G/ACGTC
TGCA- 3′ timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered blue white
PvuICGATCG
CGAT/CG
GC/TAGC
AT- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
PvuI-HF®CGATCG
CGAT/CG
GC/TAGC
AT- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
PvuIICAGCTG
CAG/CTG
GTC/GAC
blunt timesaver 5min recombinant cloned at neb heat inactivation no icon_neb3_1 incubation temp
PvuII-HF®CAGCTG
CAG/CTG
GTC/GAC
blunt timesaver 5min recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp engineered
SacIGAGCTC
GAGCT/C
C/TCGAG
AGCT- 3′ timesaver 5min recombinant cloned at neb heat inactivation yes icon_neb1_1 incubation temp blue white
SacI-HF®GAGCTC
GAGCT/C
C/TCGAG
AGCT- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
SacIICCGCGG
CCGC/GG
GG/CGCC
GC- 3′ timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
SalIGTCGAC
G/TCGAC
CAGCT/G
5′ - TCGA timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_neb3_1 incubation temp blue white
SalI-HF®GTCGAC
G/TCGAC
CAGCT/G
5′ - TCGA timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
SalI-HF® RE-Mix®GTCGAC
G/TCGAC
CAGCT/G
5′ - TCGA timesaver 5min cpg recombinant cloned at neb heat inactivation yes incubation temp engineered
ScaIAGTACT
AGT/ACT
TCA/TGA
blunt recombinant cloned at neb heat inactivation yes NEBuffer 3 incubation temp
ScaI-HF®AGTACT
AGT/ACT
TCA/TGA
blunt timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered
ScaI-HF® RE-Mix®AGTACT
AGT/ACT
TCA/TGA
blunt timesaver 5min recombinant cloned at neb heat inactivation yes incubation temp engineered
SfcICTRYAG
C/TRYAG
GAYRT/C
5′ - TRYA recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
SfoIGGCGCC
GGC/GCC
CCG/CGG
blunt timesaver 5min cpg dcm recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
SmaICCCGGG
CCC/GGG
GGG/CCC
blunt timesaver 5min cpg recombinant cloned at neb heat inactivation yes icon_CutSmart 25 degree incubation temp blue white
SmlICTYRAG
C/TYRAG
GARYT/C
5′ - TYRA recombinant cloned at neb heat inactivation no icon_CutSmart incubation temp
SnaBITACGTA
TAC/GTA
ATG/CAT
blunt cpg recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp
SpeIACTAGT
A/CTAGT
TGATC/A
5′ - CTAG timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp blue white
SpeI RE-Mix®ACTAGT
A/CTAGT
TGATC/A
5′ - CTAG timesaver 5min recombinant cloned at neb heat inactivation yes incubation temp
SpeI-HF®ACTAGT
A/CTAGT
TGATC/A
5′ - CTAG timesaver 5min recombinant cloned at neb heat inactivation yes icon_CutSmart incubation temp engineered blue white
SphIGCATGC
GCATG/C
C/GTACG
CATG- 3′ recombinant cloned at neb heat inactivation yes icon_neb2_1 incubation temp blue white
SphI-HF®GCATGC
GCATG/C
C/GTACG
CATG- 3′ timesaver 5min star activity