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  • Double Digest Finder

    Use this tool to guide your reaction buffer selection when setting up double digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Learn more information about double digests, including how to set up a reaction.

    EnzymeCat #TempSupplied NEBufferSupplements% Activity in NEBuffer
    BamHItimesaver 5minheat inactivation noR013637°CNEBuffer 3.1no75*100*100100*
    BglIItimesaver 5minheat inactivation noR014437°CNEBuffer 3.1no101010010

    Double Digest Recommendations for BamHI + BglII:

    • Digest in NEBuffer 3.1 at 37°C.
    • BamHI has a High Fidelity version BamHI-HF® (R3136)
      High Fidelity (HF) Restriction Enzymes have been engineered for reduced star activity and have 100% activity in CutSmart Buffer which may simplify your double digest.
    • * May exhibit star activity in this buffer.
    We also encourage you to use our online tool, NEBcloner, which provides you with a detailed double-digest protocol based upon the 2 restriction enzymes that you select.
    NEB Cloner