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  • Double Digest Finder

    Use this tool to guide your reaction buffer selection when setting up double digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Learn more information about double digests, including how to set up a reaction.

    EnzymeCat #TempSupplied NEBufferSupplements% Activity in NEBuffer
    SAM1.12.13.1CutSmart
    BamHItimesaver 5minheat inactivation noR013637°CNEBuffer 3.1no75*100*100100*
    BglIItimesaver 5minheat inactivation noR014437°CNEBuffer 3.1no101010010

    Double Digest Recommendations for BamHI + BglII:

    • Digest in NEBuffer 3.1 at 37°C.
    • BamHI has a High Fidelity version BamHI-HF® (R3136)
      High Fidelity (HF) Restriction Enzymes have been engineered for reduced star activity and have 100% activity in CutSmart Buffer which may simplify your double digest.
    • * May exhibit star activity in this buffer.
    The Double Digest Finder now contains information on the new buffer system. However, we understand that many customers will still have enzymes that were provided with the old buffer system and will thus still need to reference this information. The “old” Double Digest Finder is available on each restriction enzyme product page. You can get additional support by contacting info@neb.com.

    Learn more about improvements to the NEB Restriction Enzyme Buffer System