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  • Double Digest Finder

    We have exciting news! We have transitioned our restriction enzymes into a new buffer system in order to make your restriction digests even more convenient for you. This latest improvement involves the addition of BSA, as well as the removal of DTT. By adding BSA to the buffer, we are now able to offer >200 enzymes that cut in a single buffer. This improves ease-of-use, especially when performing double digests. It also eliminates the need to add BSA separately. NEBuffers 1, 2 and 3 have been replaced by NEBuffers 1.1, 2.1 and 3.1, respectively. NEBuffer 4 has been replaced with CutSmart Buffer. Please note that the old buffers are still available. Detailed information on the new buffer system, along with an extensive list of FAQs, can be found at NEBCutSmart.com.

    The Double Digest Finder now contains information on the new buffer system. However, we understand that many customers will still have enzymes that were provided with the old buffer system and will thus still need to reference this information. The “old” Double Digest Finder is available on each restriction enzyme product page. You can get additional support by contacting info@neb.com.

    Use this tool to guide your reaction buffer selection when setting up double digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.

    Learn more information about double digests, including how to set up a reaction.

    The old Activity/Performance Chart is available on each restriction enzyme product page and from the new NEBuffer Activity/Performance Chart.

    Learn more about improvements to the NEB Restriction Enzyme Buffer System