Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card.
Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.