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  • Restriction Enzyme Diluent Buffer Compatibility

    Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

    Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.

    Diluent Buffer A
    Acc65IAccIAciIAflIIAgeI-HF (2)
    AhdIAlwIAlwNIApaIApaLI
    ApoIAscI (2)AvaIAvaIIBaeGI
    BaeIBamHI (1)BamHI-HF®BanIBanII
    BccIBceAIBcgIBclIBglII
    BlpIBsaHIBsaJIBsaWIBseRI
    BsiEIBslIBsmFIBsmIBsoBI
    Bsp1286IBspCNIBspDIBspHIBsrBI
    BsrDIBsrGIBssKIBstAPIBstBI
    BstEII  (1) (2)BstEII-HF®BstNIBstUIBstYI
    Bsu36IBtgZIBtsIBtsIMutIClaI
    CspCICviKI-1DraIDrdIEaeI
    EciIEco53kIEcoNIEcoO109IEcoP15I
    EcoRV (1) (2)FatIFauIFnu4HIFokI
    HaeIIHaeIIIHgaIHhaIHinfI
    HinP1IHpaIHpaIIHpy188IHpy99I
    HpyCH4IIIHpyCH4IVHpyCH4VKpnI (1) (2)KpnI-HF® (2)
    MboIMfeI (1) (2)MfeI-HF® (2)MluCIMluI
    MlyIMseIMslIMspINaeI
    NarINb.BbvCINb.BsmINb.BsrDINb.BtsI
    NciINcoI (1) (2)NdeINgoMIVNotI-HF® (2)
    NruINspINt.AlwINt.BbvCINt.BsmAI
    Nt.BstNBINt.CviPIIPacI (2)PaeR7IPflFI
    PflMIPhoIPleIPluTIPmeI
    PmlIPshAIPspGIRsaISacI (1)
    SacI-HF®SacIISalI (1) (2)SalI-HF® (2)Sau3AI
    Sau96ISbfI (1)ScaI (1) (2)SexAISgrAI
    SmlISnaBIStuIStyI (1)StyI-HF®
    Tsp45ITsp509IXbaI (2)XhoI (2)XmaI
    XmnI
    Diluent Buffer B
    AatIIAclIAcuIAfeIAflIII
    AleIAluIApeKIAseIAsiSI
    AvrIIBbsIBbvCIBbvIBcoDI
    BfaIBfuAIBfuCIBglIBmgBI
    BmrIBmtI (1)BmtI-HF®BpmIBpu10I
    BpuEIBsaBIBsaI (1)BsaI-HF®BsaXI
    BseYIBsgIBsiHKAIBsiWIBsmAI
    BsmBIBspEIBspMIBspQIBsrI
    BssHIIBssSIBstXIBstZ17IBtgI
    BtsCICac8IDdeIDpnIDpnII
    DraIII (1)DraIII-HF®EagI-HF®EarIEcoRV-HF® (2)
    FseIFspEIHincIIHindIII (1)HindIII-HF®
    HphIHpy188IIIHpyAVI-CeuII-SceI
    KasILpnPIMmeIMnlIMscI
    MspA1IMspJIMwoINcoI-HF® (2)NlaIII
    NlaIVNmeAIIINsiINt.BspQIPciI
    PI-PspIPI-SceIPpuMIPsiIPspOMI
    PspXIPvuI (1)PvuI-HF®PvuII (1)PvuII-HF®
    SapISbfI-HF®ScaI-HF® (2)SfaNISfcI
    SfoISmaISphI (1)SphI-HF®SspI-HF®
    StyD4ISwaITaqαITliITseI
    TspMITspRITth111IZraI
    Diluent Buffer C
    AbaSIAgeI (1) (2)BciVIBsaAIBsrFI
    CviAIICviQIEagI (1)EcoRI (1) (2)EcoRI-HF® (2)
    FspIHpy166IIMboIINheI (1) (2)NheI-HF® (2)
    NotI (1) (2)PstI (1)PstI-HF®RsrIIScrFI
    SfiISpeI (1) (2)SpeI-HF®SspI (1)TfiI
    XcmI
    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.
    HF® is a registered trademark of New England Biolabs, Inc.