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  • Restriction Enzyme Diluent Buffer Compatibility

    Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

    Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.

    Diluent Buffer A
    Acc65IAccIAciIAflIIAgeI-HF® (2)
    AhdIAlwIAlwNIApaIApaLI
    ApoI (1)AscI (2)AvaIAvaIIBaeGI
    BaeIBamHI (1)BamHI-HF®BanIBanII
    BccIBceAIBcgIBclIBglII
    BlpIBsaJIBsaWIBseRIBsiEI
    BsiHKAIBslIBsmFIBsmIBsoBI
    Bsp1286IBspCNIBspDIBspHIBsrBI
    BsrDIBsrGI (1)BsrGI-HF®BssKIBstAPI
    BstBIBstEII  (1) (2)BstEII-HF®BstNIBstUI
    BstYIBstZ17I-HF®BtgZIBtsαIBtsI
    BtsIMutIClaICspCICviKI-1DraI
    DrdIEaeIEciIEco53kIEcoNI
    EcoO109IEcoP15IEcoRV (1) (2)FatIFauI
    Fnu4HIFokIHaeIIHaeIIIHgaI
    HhaIHinfIHinP1IHpaIHpaII
    Hpy188IHpy99IHpyCH4IIIHpyCH4IVHpyCH4V
    KpnI (1) (2)KpnI-HF® (2)MboIMfeI (1) (2)MfeI-HF® (2)
    MluCIMluI (1)MluI-HF®MlyIMseI
    MslIMspINaeINarINb.BbvCI
    Nb.BsmINb.BsrDINb.BtsINciINcoI (1) (2)
    NdeINgoMIVNotI-HF® (2)NruI (1)NruI-HF®
    NspINt.AlwINt.BbvCINt.BsmAINt.BstNBI
    Nt.CviPIIPacI (2)PaeR7IPflFIPflMI
    PhoIPleIPluTIPmeIPmlI
    PshAIPspGIRsaISacI (1)SacI-HF®
    SacIISalI (1) (2)SalI-HF® (2)Sau3AISau96I
    SbfI (1)ScaI (1) (2)SexAISgrAISmlI
    SnaBIStuIStyI (1)StyI-HF®Tsp45I
    Tsp509IXbaI (2)XhoI (2)XmaIXmnI
    Diluent Buffer B
    AatIIAclIAcuIAfeIAflIII
    AleIAluIApeKIApoI-HFAseI
    AsiSIAvrIIBbsIBbsI-HF®BbvCI
    BbvIBcoDIBfaIBfuAIBfuCI
    BglIBmgBIBmrIBmtI (1)BmtI-HF®
    BpmIBpu10IBpuEIBsaBIBsaI (1)
    BsaI-HF®BseYIBsgIBsiWIBsiWI-HF®
    BsmAIBsmBIBspEIBspMIBspQI
    BsrIBssHIIBssSαIBssSIBstXI
    BstZ17IBtgIBtsCICac8IDdeI
    DpnIDpnIIDraIII (1)DraIII-HF®EagI (1)
    EagI-HF®EarIEcoRV-HF® (2)FseIFspEI
    HincIIHindIII (1)HindIII-HF®HphIHpy188III
    I-CeuII-SceIKasILpnPIMmeI
    MnlIMspA1IMspJIMwoINb.BssSI
    NcoI-HF® (2)NlaIIINlaIVNmeAIIINsiI (1)
    NsiI-HF®Nt.BspQIPciIPI-PspIPI-SceI
    PpuMIPsiIPspOMIPspXIPvuI (1)
    PvuI-HF®PvuII (1)PvuII-HF®SapISbfI-HF®
    ScaI-HF® (2)SfaNISfcISfoISmaI
    SphI (1)SphI-HF®SrfISspI-HF®StyD4I
    SwaITaqαITliITseITspMI
    TspRITth111IZraI
    Diluent Buffer C
    AbaSIAgeI (1) (2)BciVIBsaAIBsaHI
    BsaXIBsrFαIBsrFIBsu36ICviAII
    CviQIEcoRI (1) (2)EcoRI-HF® (2)FspIHpy166II
    MboIIMscINheI (1) (2)NheI-HF® (2)NotI (1) (2)
    PstI (1)PstI-HF®RsrIIScrFISfiI
    SpeI (1) (2)SpeI-HF®SspI (1)TfiIXcmI
    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.
    HF® is a registered trademark of New England Biolabs, Inc.