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  • Restriction Enzyme Diluent Buffer Compatibility

    Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

    Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.

    Diluent Buffer A
    Acc65IAccIAciIAflIIAgeI-HF® (2)
    AhdIAlwIAlwNIApaIApaLI
    ApoI (1)AscI (2)AvaIAvaIIBaeGI
    BaeIBamHI (1)BamHI-HF®BanIBanII
    BccIBceAIBcgIBclIBglII
    BlpIBsaHIBsaJIBsaWIBseRI
    BsiEIBslIBsmFIBsmIBsoBI
    Bsp1286IBspCNIBspDIBspHIBsrBI
    BsrDIBsrGI (1)BsrGI-HF®BssKIBstAPI
    BstBIBstEII  (1) (2)BstEII-HF®BstNIBstUI
    BstYIBtgZIBtsαIBtsIBtsIMutI
    ClaICspCICviKI-1DraIDrdI
    EaeIEciIEco53kIEcoNIEcoO109I
    EcoP15IEcoRV (1) (2)FatIFauIFnu4HI
    FokIHaeIIHaeIIIHgaIHhaI
    HinfIHinP1IHpaIHpaIIHpy188I
    Hpy99IHpyCH4IIIHpyCH4IVHpyCH4VKpnI (1) (2)
    KpnI-HF® (2)MboIMfeI (1) (2)MfeI-HF® (2)MluCI
    MluI (1)MluI-HF®MlyIMseIMslI
    MspINaeINarINb.BbvCINb.BsmI
    Nb.BsrDINb.BtsINciINcoI (1) (2)NdeI
    NgoMIVNotI-HF® (2)NruI (1)NruI-HF®NspI
    Nt.AlwINt.BbvCINt.BsmAINt.BstNBINt.CviPII
    PacI (2)PaeR7IPflFIPflMIPhoI
    PleIPluTIPmeIPmlIPshAI
    PspGIRsaISacI (1)SacI-HF®SacII
    SalI (1) (2)SalI-HF® (2)Sau3AISau96ISbfI (1)
    ScaI (1) (2)SexAISgrAISmlISnaBI
    StuIStyI (1)StyI-HF®Tsp45ITsp509I
    XbaI (2)XhoI (2)XmaIXmnI
    Diluent Buffer B
    AatIIAclIAcuIAfeIAflIII
    AleIAluIApeKIApoI-HFAseI
    AsiSIAvrIIBbsIBbvCIBbvI
    BcoDIBfaIBfuAIBfuCIBglI
    BmgBIBmrIBmtI (1)BmtI-HF®BpmI
    Bpu10IBpuEIBsaBIBsaI (1)BsaI-HF®
    BsaXIBseYIBsgIBsiHKAIBsiWI
    BsmAIBsmBIBspEIBspMIBspQI
    BsrIBssHIIBssSαIBssSIBstXI
    BstZ17IBtgIBtsCICac8IDdeI
    DpnIDpnIIDraIII (1)DraIII-HF®EagI-HF®
    EarIEcoRV-HF® (2)FseIFspEIHincII
    HindIII (1)HindIII-HF®HphIHpy188IIII-CeuI
    I-SceIKasILpnPIMmeIMnlI
    MscIMspA1IMspJIMwoINcoI-HF® (2)
    NlaIIINlaIVNmeAIIINsiI (1)NsiI-HF®
    Nt.BspQIPciIPI-PspIPI-SceIPpuMI
    PsiIPspOMIPspXIPvuI (1)PvuI-HF®
    PvuII (1)PvuII-HF®SapISbfI-HF®ScaI-HF® (2)
    SfaNISfcISfoISmaISphI (1)
    SphI-HF®SrfISspI-HF®StyD4ISwaI
    TaqαITliITseITspMITspRI
    Tth111IZraI
    Diluent Buffer C
    AbaSIAgeI (1) (2)BciVIBsaAIBsrFI
    Bsu36ICviAIICviQIEagI (1)EcoRI (1) (2)
    EcoRI-HF® (2)FspIHpy166IIMboIINheI (1) (2)
    NheI-HF® (2)NotI (1) (2)PstI (1)PstI-HF®RsrII
    ScrFISfiISpeI (1) (2)SpeI-HF®SspI (1)
    TfiIXcmI
    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.
    HF® is a registered trademark of New England Biolabs, Inc.