Chart Legend

U

Supplied with its own unique reaction buffer that is different from the four standard NEBuffers. Its compatibility with the four standard NEBuffers is indicated by the chart.

BSA

Supplied with a separate vial of bovine serum albumin (10 mg/ml). To obtain 100% activity BSA should be added to the 1X reaction mix to a final concentration of 100 µg/ml.

SAM

Supplied with a separate vial of S-adenosyl-methionine (SAM). To obtain 100% activity, SAM should be added to the 1X reaction mix as specified on the product data card.

dd

When performing a double digest with this enzyme, this NEBuffer is recommended because it minimizes star activity.

*

Purified by scientists at SibEnzyme and supplied with a SibEnzyme buffer (B, K, O, W or Y) which ensures 100% activity. Its compatibility with the NEBuffer System is indicated on the chart.

N/R

This buffer is not recommended for use with with this enzyme.

Buffer Compositions (1X):

NEBuffer 1:
10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).

NEBuffer 2:
10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 3:
50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 4:
20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).