We have exciting news! We have transitioned our restriction enzymes into a new buffer system in order to make your restriction digests even more convenient for you. This latest improvement involves the addition of BSA, as well as the removal of DTT. By adding BSA to the buffer, we are now able to offer >200 enzymes that cut in a single buffer. This improves ease-of-use, especially when performing double digests. It also eliminates the need to add BSA separately. NEBuffers 1, 2 and 3 have been replaced by NEBuffers 1.1, 2.1 and 3.1, respectively. NEBuffer 4 has been replaced with CutSmart Buffer. Please note that the old buffers are still available. Detailed information on the new buffer system, along with an extensive list of FAQs, can be found at NEBCutSmart.com.
The Activity/Performance now contains information on the new buffer system. However, we understand that many customers will still have enzymes that were provided with the old buffer system and will thus still need to reference this information. The old NEBuffer Activity Chart is available on each RE product page. You can also receive additional support by contacting firstname.lastname@example.org.
Please check out other technical reference information related to restriction enzymes:
Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes
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The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.