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  • NEBuffer Activity/Performance Chart with Restriction Enzymes

    We have exciting news! We have transitioned our restriction enzymes into a new buffer system in order to make your restriction digests even more convenient for you. This latest improvement involves the addition of BSA, as well as the removal of DTT. By adding BSA to the buffer, we are now able to offer >200 enzymes that cut in a single buffer. This improves ease-of-use, especially when performing double digests. It also eliminates the need to add BSA separately. NEBuffers 1, 2 and 3 have been replaced by NEBuffers 1.1, 2.1 and 3.1, respectively. NEBuffer 4 has been replaced with CutSmart Buffer. Please note that the old buffers are still available. Detailed information on the new buffer system, along with an extensive list of FAQs, can be found at NEBCutSmart.com.

    The Activity/Performance now contains information on the new buffer system. However, we understand that many customers will still have enzymes that were provided with the old buffer system and will thus still need to reference this information. The old NEBuffer Activity Chart is available on each RE product page. You can also receive additional support by contacting info@neb.com.

    Legend

    Not Sensitive   Impaired
    Blocked Impaired by Overlapping
    Blocked by Overlapping Impaired by Some Combinations of Overlapping
    Blocked by Some Combinations of Overlapping

    Please check out other technical reference information related to restriction enzymes:
    Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes 

    Chart Legend | Icon Descriptions

    EnzymeSequenceSupplied NEBuffer% Activity in NEBufferHeat Inac.Incu. Temp.DiluentDamDcmCpGUnit SubstrateNote
    1.12.13.1CutSmart
    AatII recombinant timesaver 5min cpgGACGT/CCutSmart™ Buffer1050*5010080°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    AbaSI recombinantNEBuffer 425505010065°C25°CNot SensitiveNot SensitiveNot Sensitivee
    Acc65I recombinant timesaver 5min dcm cpgG/GTACCNEBuffer 3.11075*1002565°C37°CNot SensitiveBlocked by Some Combinations of OverlappingBlocked by Some Combinations of OverlappingpBC4 DNA
    AccI recombinant timesaver 5min cpgGT/MKACCutSmart™ Buffer50501010080°C37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNA
    AciI recombinant timesaver 5min cpgCCGC(-3/-1)CutSmart™ Buffer102510010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    AclI recombinant timesaver 5min cpgAA/CGTTCutSmart™ Buffer101010100No37°CNot SensitiveNot SensitiveBlockedλ DNA
    AcuI recombinant timesaver 5minCTGAAG(16/14)CutSmart™ Buffer + SAM501005010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3, b, d
    AfeI recombinant cpgAGC/GCTCutSmart™ Buffer251002510065°C37°CNot SensitiveNot SensitiveBlockedpXba DNA
    AflII recombinant timesaver 5minC/TTAAGCutSmart™ Buffer501001010065°C37°CNot SensitiveNot SensitiveNot SensitiveΦX174 RF I DNA
    AflIII recombinantA/CRYGTNEBuffer 3.110501005080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    AgeI recombinant cpgA/CCGGTNEBuffer 1.110075257565°C37°CNot SensitiveNot SensitiveBlockedλ DNA2
    AgeI-HF recombinant timesaver 5min engineered cpgA/CCGGTCutSmart™ Buffer100501010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    AgeI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix icon cpgA/CCGGT-----65°C37°C-Not SensitiveNot SensitiveBlocked
    AhdI recombinant timesaver 5min cpgGACNNN/NNGTCCutSmart™ Buffer25251010065°C37°CNot SensitiveNot SensitiveImpaired by Some Combinations of Overlappingλ DNAa
    AleI recombinant cpgCACNN/NNGTGCutSmart™ Buffer10101010080°C37°CNot SensitiveNot SensitiveImpaired by Some Combinations of Overlappingλ DNA
    AluI recombinant timesaver 5minAG/CTCutSmart™ Buffer251005010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAb
    AlwI recombinant damGGATC(4/5)CutSmart™ Buffer505010100No37°CBlockedNot SensitiveNot Sensitiveλ DNA (dam-)1, b, d
    AlwNI recombinant timesaver 5min dcmCAGNNN/CTGCutSmart™ Buffer101005010080°C37°CNot SensitiveBlocked by OverlappingNot Sensitiveλ DNA
    ApaI recombinant timesaver 5min dcm cpgGGGCC/CCutSmart™ Buffer25251010065°C25°CNot SensitiveBlocked by OverlappingBlocked by OverlappingpXba DNA
    ApaLI recombinant timesaver 5min cpgG/TGCACCutSmart™ Buffer10010010100No37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNA (HindIII digest)
    ApeKI recombinant timesaver 5min cpgG/CWGCNEBuffer 3.1255010010No75°CNot SensitiveNot Sensitiveλ DNA
    ApoI recombinant timesaver 5minR/AATTYNEBuffer 3.110751007580°C50°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    AscI recombinant timesaver 5min cpgGG/CGCGCCCutSmart™ Buffer10101010080°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    AscI RE-Mix® recombinant timesaver 5min re-mix icon cpgGG/CGCGCC-----65°C37°C-Not SensitiveNot SensitiveBlocked
    AseI recombinant timesaver 5minAT/TAATNEBuffer 3.11050*1001065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3
    AsiSI recombinant cpgGCGAT/CGCCutSmart™ Buffer5010010010080°C37°CNot SensitiveNot SensitiveBlockedXhoI digested pXba2, b
    AvaI recombinant timesaver 5min cpgC/YCGRGCutSmart™ Buffer101002510080°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    AvaII recombinant timesaver 5min dcm cpgG/GWCCCutSmart™ Buffer50751010080°C37°CNot SensitiveBlocked by OverlappingBlocked by Overlappingλ DNA
    AvrII recombinant timesaver 5minC/CTAGGCutSmart™ Buffer1005050100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA (HindIII digest)
    BaeGI timesaver 5minGKGCM/CNEBuffer 3.175751002580°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BaeI timesaver 5min cpg(10/15)ACNNNNGTAYC(12/7)CutSmart™ Buffer + SAM501005010065°C25°CNot SensitiveNot SensitiveBlocked by Some Combinations of OverlappingpXba DNAe
    BamHI recombinant timesaver 5minG/GATCCNEBuffer 3.175*100*100100*No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3
    BamHI-HF® recombinant timesaver 5min engineeredG/GATCCCutSmart™ Buffer1005010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BanI recombinant dcm cpgG/GYRCCCutSmart™ Buffer10251010065°C37°CNot SensitiveBlocked by Some Combinations of OverlappingBlocked by Some Combinations of Overlappingλ DNA1
    BanII recombinantGRGCY/CCutSmart™ Buffer1001005010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA2
    BbsI timesaver 5minGAAGAC(2/6)NEBuffer 2.110010025N/R65°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BbvCI recombinant cpgCCTCAGC(-5/-2)CutSmart™ Buffer1010050100No37°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNA1, a
    BbvI recombinant timesaver 5minGCAGC(8/12)CutSmart™ Buffer1001002510065°C37°CNot SensitiveNot SensitiveNot SensitivepBR322 (dcm+) DNA3
    BccI recombinantCCATC(4/5)CutSmart™ Buffer100501010065°C37°CNot SensitiveNot SensitiveNot SensitivepXba DNA3, b
    BceAI recombinant cpgACGGC(12/14)NEBuffer 3.1100*100*100100*65°C37°CNot SensitiveNot SensitiveBlockedpBR322 (dcm+) DNA1
    BcgI recombinant dam cpg(10/12)CGANNNNNNTGC(12/10)NEBuffer 3.1 + SAM1075*10050*65°C37°CImpaired by Overlapping Not SensitiveBlocked by Some Combinations of Overlappingλ DNAe
    BciVI recombinant timesaver 5minGTATCC(6/5)CutSmart™ Buffer100251010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAb
    BclI recombinant timesaver 5min damT/GATCANEBuffer 3.15010010075No50°CBlockedNot SensitiveNot Sensitiveλ DNA (dam-)
    BcoDI recombinant timesaver 5min cpgGTCTC(1/5)CutSmart™ Buffer507575100No37°CNot SensitiveNot SensitiveImpaired by Some Combinations of Overlappingλ DNA
    BfaIC/TAGCutSmart™ Buffer10101010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA2, b
    BfuAI timesaver 5min cpgACCTGC(4/8)NEBuffer 3.110251001065°C50°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNA3
    BfuCI timesaver 5min cpg/GATCCutSmart™ Buffer100502510080°C37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNA
    BglI recombinant timesaver 5min cpgGCCNNNN/NGGCNEBuffer 3.110251001065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    BglII recombinant timesaver 5minA/GATCTNEBuffer 3.1101010010No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BlpI recombinant timesaver 5minGC/TNAGCCutSmart™ Buffer5010010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAd
    BmgBI recombinant timesaver 5min cpgCACGTC(-3/-3)NEBuffer 3.110101001065°C37°CNot SensitiveNot SensitiveBlockedλ DNA3, b, d
    BmrI recombinantACTGGG(5/4)NEBuffer 2.17510075100*65°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA (HindIII digest)b
    BmtI recombinantGCTAG/CNEBuffer 3.110010010010065°C37°CNot SensitiveNot SensitiveNot SensitivepXba DNA2
    BmtI-HF® recombinant timesaver 5min engineeredGCTAG/CCutSmart™ Buffer501001010065°C37°CNot SensitiveNot SensitiveNot SensitivepXba DNA
    BpmI recombinantCTGGAG(16/14)NEBuffer 3.17510010010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA2
    Bpu10I recombinantCCTNAGC(-5/-2)NEBuffer 3.110251002580°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3, b, d
    BpuEI recombinant timesaver 5minCTTGAG(16/14)CutSmart™ Buffer + SAM50*10050*10065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAd
    BsaAI recombinant timesaver 5min cpgYAC/GTRCutSmart™ Buffer100100100100No37°CNot SensitiveNot SensitiveBlockedλ DNA
    BsaBI dam cpgGATNN/NNATCCutSmart™ Buffer501007510080°C60°CBlocked by OverlappingNot SensitiveBlocked by Some Combinations of Overlappingλ DNA (dam-)2
    BsaHI recombinant timesaver 5min dcm cpgGR/CGYCCutSmart™ Buffer5010010010080°C37°CNot SensitiveBlocked by Some Combinations of OverlappingBlockedλ DNA
    BsaI recombinant dcm cpgGGTCTC(1/5)CutSmart™ Buffer75*7510010065°C37°CNot SensitiveBlocked by OverlappingBlocked by Some Combinations of OverlappingpXba DNA3
    BsaI-HF® recombinant timesaver 5min engineered dcm cpgGGTCTC(1/5)CutSmart™ Buffer501002510065°C37°CNot SensitiveBlocked by OverlappingBlocked by Some Combinations of OverlappingpXba DNA
    BsaJI recombinantC/CNNGGCutSmart™ Buffer5010010010080°C60°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BsaWI recombinant timesaver 5minW/CCGGWCutSmart™ Buffer101005010080°C60°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BsaXI timesaver 5min(9/12)ACNNNNNCTCC(10/7)CutSmart™ Buffer50*100*10100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAe
    BseRI recombinant timesaver 5minGAGGAG(10/8)CutSmart™ Buffer100*1007510080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAd
    BseYI recombinant cpgCCCAGC(-5/-1)NEBuffer 3.110501005080°C37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNAd
    BsgI recombinant timesaver 5minGTGCAG(16/14)CutSmart™ Buffer + SAM25502510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAd
    BsiEI timesaver 5min cpgCGRY/CGCutSmart™ Buffer25501010080°C60°CNot SensitiveNot SensitiveBlockedλ DNA
    BsiHKAIGWGCW/CCutSmart™ Buffer25100100100No65°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BsiWI recombinant timesaver 5min cpgC/GTACGNEBuffer 3.12550*1002565°C55°CNot SensitiveNot SensitiveBlockedΦX174 DNA
    BslI recombinant timesaver 5min dcm cpgCCNNNNN/NNGGCutSmart™ Buffer5075100100No55°CNot SensitiveBlocked by Some Combinations of OverlappingBlocked by Some Combinations of Overlappingλ DNAb
    BsmAI recombinant timesaver 5min cpgGTCTC(1/5)CutSmart™ Buffer50100100100No55°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    BsmBI recombinant timesaver 5min cpgCGTCTC(1/5)NEBuffer 3.11050*1002580°C55°CNot SensitiveNot SensitiveBlockedλ DNA
    BsmFI recombinant dcm cpgGGGAC(10/14)CutSmart™ Buffer25505010080°C65°CNot SensitiveBlocked by OverlappingBlocked by OverlappingpBR322 (dcm+) DNA1
    BsmI recombinant timesaver 5minGAATGC(1/-1)CutSmart™ Buffer251001010080°C65°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BsoBI recombinant timesaver 5minC/YCGRGCutSmart™ Buffer2510010010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    Bsp1286I recombinant timesaver 5minGDGCH/CCutSmart™ Buffer25252510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3
    BspCNI recombinant timesaver 5minCTCAG(9/7)CutSmart™ Buffer + SAM100751010080°C25°CNot SensitiveNot SensitiveNot Sensitiveλ DNAb
    BspDI recombinant dam cpgAT/CGATCutSmart™ Buffer25755010080°C37°CBlocked by OverlappingNot SensitiveBlockedλ DNA
    BspEI recombinant timesaver 5min dam cpgT/CCGGANEBuffer 3.110101001080°C37°CBlocked by OverlappingNot SensitiveImpairedλ DNA (dam-)
    BspHI recombinant timesaver 5min damT/CATGACutSmart™ Buffer10502510080°C37°CBlocked by OverlappingNot SensitiveNot Sensitiveλ DNA
    BspMI recombinantACCTGC(4/8)NEBuffer 3.11050*1001065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BspQI recombinant timesaver 5minGCTCTTC(1/4)NEBuffer 3.110010010010080°C50°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3
    BsrBI timesaver 5min cpgCCGCTC(-3/-3)CutSmart™ Buffer5010010010080°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNAd
    BsrDI timesaver 5minGCAATG(2/0)NEBuffer 2.110100752580°C65°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3, d
    BsrFI recombinant cpgR/CCGGYCutSmart™ Buffer10100*100*100No37°CNot SensitiveNot SensitiveBlockedpBR322 (dcm+) DNA1
    BsrGI recombinant timesaver 5minT/GTACANEBuffer 2.1251001002580°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BsrI timesaver 5minACTGG(1/-1)NEBuffer 3.110501001080°C65°CNot SensitiveNot SensitiveNot SensitiveΦX174 RF I DNAb
    BssHII recombinant timesaver 5min cpgG/CGCGCCutSmart™ Buffer10010010010065°C50°CNot SensitiveNot SensitiveBlockedλ DNA
    BssKI recombinant timesaver 5min dcm cpg/CCNGGCutSmart™ Buffer5010010010080°C60°CNot SensitiveBlocked by OverlappingBlocked by Overlappingλ DNAb
    BssSI recombinantCACGAG(-5/-1)NEBuffer 3.1501001005080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BstAPI recombinant cpgGCANNNN/NTGCCutSmart™ Buffer501002510080°C60°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNAb
    BstBI recombinant timesaver 5min cpgTT/CGAACutSmart™ Buffer7510010100No65°CNot SensitiveNot SensitiveBlockedλ DNA
    BstEII  recombinant timesaver 5minG/GTNACCNEBuffer 3.11075*10075*No60°CNot SensitiveNot SensitiveNot Sensitiveλ DNA3
    BstEII-HF® recombinant timesaver 5min engineeredG/GTNACCCutSmart™ Buffer101010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BstEII-HF® RE-Mix® recombinant timesaver 5min engineered re-mix iconG/GTNACC-----No37°C-Not SensitiveNot SensitiveNot Sensitive
    BstNI recombinant timesaver 5minCC/WGGNEBuffer 3.11010010075No60°CNot SensitiveNot SensitiveNot Sensitiveλ DNAa
    BstUI timesaver 5min cpgCG/CGCutSmart™ Buffer5010025100No60°CNot SensitiveNot SensitiveBlockedλ DNAb
    BstXI recombinant timesaver 5min dcmCCANNNNN/NTGGNEBuffer 3.110501002580°C37°CNot SensitiveBlocked by Some Combinations of OverlappingNot Sensitiveλ DNA3
    BstYI recombinant timesaver 5minR/GATCYNEBuffer 2.12510075100No60°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BstZ17I timesaver 5min cpgGTA/TACCutSmart™ Buffer75100100100No37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA3, b
    Bsu36I recombinant timesaver 5minCC/TNAGGCutSmart™ Buffer2510010010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA (HindIII digest)b
    BtgI recombinant timesaver 5minC/CRYGGCutSmart™ Buffer5010010010080°C37°CNot SensitiveNot SensitiveNot SensitivepBR322 (dcm+) DNA
    BtgZI recombinant cpgGCGATG(10/14)CutSmart™ Buffer10251010080°C60°CNot SensitiveNot SensitiveImpairedλ DNA3, b, d
    BtsCI recombinant timesaver 5minGGATG(2/0)CutSmart™ Buffer101002510080°C50°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    BtsI recombinantGCAGTG(2/0)CutSmart™ Buffer100505010080°C55°CNot SensitiveNot SensitiveNot Sensitiveλ DNA1, b
    BtsIMutI recombinantCAGTG(2/0)CutSmart™ Buffer100501010080°C55°CNot SensitiveNot SensitiveNot SensitivepUC19 DNAb
    Cac8I timesaver 5min cpgGCN/NGCCutSmart™ Buffer507510010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNAb
    ClaI recombinant timesaver 5min dam cpgAT/CGATCutSmart™ Buffer10505010065°C37°CBlocked by OverlappingNot SensitiveBlockedλ DNA (dam-)
    CspCI recombinant timesaver 5min(11/13)CAANNNNNGTGG(12/10)CutSmart™ Buffer + SAM101001010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAe
    CviAII recombinant timesaver 5minC/ATGCutSmart™ Buffer50501010065°C25°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    CviKI-1 recombinantRG/CYCutSmart™ Buffer25100100100No37°CNot SensitiveNot SensitiveNot SensitivepBR322 (dcm+) DNA1, b
    CviQI recombinant timesaver 5minG/TACNEBuffer 3.175100*10075*No25°CNot SensitiveNot SensitiveNot Sensitiveλ DNAb
    DdeI recombinant timesaver 5minC/TNAGCutSmart™ Buffer7510010010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    DpnI recombinant timesaver 5min cpgGA/TCCutSmart™ Buffer1001007510080°C37°CNot SensitiveNot SensitiveBlocked by OverlappingpBR322 DNA (dam methylated)b
    DpnII recombinant timesaver 5min dam/GATCNEBuffer DpnII2525100*2565°C37°CBlockedNot SensitiveNot Sensitiveλ DNA (dam-)
    DraI recombinant timesaver 5minTTT/AAACutSmart™ Buffer75755010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    DraIII recombinant cpgCACNNN/GTGNEBuffer 3 + BSA25501002565°C37°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNA
    DraIII-HF® recombinant timesaver 5min engineered cpgCACNNN/GTGCutSmart™ Buffer105010100No37°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNAb
    DrdI timesaver 5min cpgGACNNNN/NNGTCCutSmart™ Buffer25501010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of OverlappingpUC19 DNA3
    DvuIIICACNCAC-N/RN/RN/RN/RNo
    EaeI recombinant dcm cpgY/GGCCRCutSmart™ Buffer10501010065°C37°CNot SensitiveBlocked by OverlappingBlocked by Overlappingλ DNAb
    EagI recombinant timesaver 5min cpgC/GGCCGNEBuffer 3.110251001065°C37°CNot SensitiveNot SensitiveBlockedpXba DNA
    EagI-HF® recombinant timesaver 5min engineered cpgC/GGCCGCutSmart™ Buffer2510010010065°C37°CNot SensitiveNot SensitiveBlockedpXba DNA
    EarI recombinant timesaver 5min cpgCTCTTC(1/4)CutSmart™ Buffer50101010065°C37°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNAb, d
    EciI recombinant cpgGGCGGA(11/9)CutSmart™ Buffer100505010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA2
    Eco53kI recombinant timesaver 5min cpgGAG/CTCCutSmart™ Buffer1001001010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of OverlappingpXba DNA3, b
    EcoNI recombinant timesaver 5minCCTNN/NNNAGGCutSmart™ Buffer501007510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNAb
    EcoO109I recombinant timesaver 5min dcmRG/GNCCYCutSmart™ Buffer501005010065°C37°CNot SensitiveBlocked by OverlappingNot Sensitiveλ DNA (HindIII digest)3
    EcoP15I recombinant timesaver 5minCAGCAG(25/27)NEBuffer 3.1 + ATP7510010010065°C37°CNot SensitiveNot SensitiveNot SensitivepUC19 DNAe
    EcoRI recombinant timesaver 5min cpgG/AATTCNEBuffer EcoRI25100*5050*65°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    EcoRI-HF® recombinant timesaver 5min engineered cpgG/AATTCCutSmart™ Buffer101001010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    EcoRI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix icon cpgG/AATTC-----65°C37°C-Not SensitiveNot SensitiveBlocked by Some Combinations of Overlapping
    EcoRV recombinant timesaver 5min cpgGAT/ATCNEBuffer 3.110501001080°C37°CNot SensitiveNot SensitiveImpaired by Some Combinations of Overlappingλ DNA
    EcoRV-HF® recombinant timesaver 5min engineered cpgGAT/ATCCutSmart™ Buffer2510010010065°C37°CNot SensitiveNot SensitiveImpaired by Some Combinations of Overlappingλ DNA
    EcoRV-HF® RE-Mix® recombinant timesaver 5min engineered re-mix icon cpgGAT/ATC-----65°C37°C-Not SensitiveNot SensitiveImpaired by Some Combinations of Overlapping
    FatI recombinant/CATGNEBuffer 2.110100505080°C55°CNot SensitiveNot SensitiveNot SensitivepUC19 DNA
    FauI recombinant cpgCCCGC(4/6)CutSmart™ Buffer100501010065°C55°CNot SensitiveNot SensitiveBlockedλ DNA3, b, d
    Fnu4HI recombinant timesaver 5min cpgGC/NGCCutSmart™ Buffer101010100No37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNAa
    FokI recombinant dcm cpgGGATG(9/13)CutSmart™ Buffer1001007510065°C37°CNot SensitiveImpaired by Overlapping Impaired by Overlapping λ DNA3, b, d
    FseI recombinant timesaver 5min dcm cpgGGCCGG/CCCutSmart™ Buffer100751010065°C37°CNot SensitiveImpaired by Some Combinations of OverlappingBlockedAdenovirus-2 DNA
    FspEI recombinantCC(12/16)NEBuffer 4 + BSA10101010065°C37°CNot SensitiveNot SensitiveNot SensitivepBR322 (dcm+) DNA2, e
    FspI recombinant timesaver 5min cpgTGC/GCACutSmart™ Buffer1010010100No37°CNot SensitiveNot SensitiveBlockedλ DNAb
    HaeII recombinant timesaver 5min cpgRGCGC/YCutSmart™ Buffer251001010080°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    HaeIII recombinant timesaver 5minGG/CCCutSmart™ Buffer501002510080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    HgaI recombinant cpgGACGC(5/10)NEBuffer 1.11001002510065°C37°CNot SensitiveNot SensitiveBlockedΦX174 DNA1
    HhaI recombinant timesaver 5min cpgGCG/CCutSmart™ Buffer2510010010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    HincII recombinant timesaver 5min cpgGTY/RACNEBuffer 3.12510010010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    HindIII recombinantA/AGCTTNEBuffer 2.125100505080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA2
    HindIII-HF® recombinant timesaver 5min engineeredA/AGCTTCutSmart™ Buffer101001010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    HinfI recombinant timesaver 5min cpgG/ANTCCutSmart™ Buffer5010010010080°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    HinP1I recombinant timesaver 5min cpgG/CGCCutSmart™ Buffer10010010010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    HpaI recombinant cpgGTT/AACCutSmart™ Buffer1075*25100No37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA1
    HpaII recombinant timesaver 5min cpgC/CGGCutSmart™ Buffer100501010080°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    HphI recombinant timesaver 5min damGGTGA(8/7)CutSmart™ Buffer50501010065°C37°CBlocked by OverlappingNot SensitiveNot Sensitiveλ DNAb, d
    Hpy166II recombinant timesaver 5min cpgGTN/NACCutSmart™ Buffer1001005010065°C37°CNot SensitiveNot SensitiveBlocked by OverlappingpBR322 DNA
    Hpy188I recombinant damTCN/GACutSmart™ Buffer251005010065°C37°CBlocked by Some Combinations of OverlappingNot SensitiveNot SensitivepBR322 (dcm+) DNA1, b
    Hpy188III recombinant dam cpgTC/NNGACutSmart™ Buffer1001001010065°C37°CBlocked by OverlappingNot SensitiveBlocked by OverlappingpUC19 DNA
    Hpy99I recombinant cpgCGWCG/CutSmart™ Buffer50101010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    HpyAV recombinant timesaver 5min cpgCCTTC(6/5)CutSmart™ Buffer1001002510065°C37°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNA
    HpyCH4III recombinantACN/GTCutSmart™ Buffer100251010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    HpyCH4IV recombinant timesaver 5min cpgA/CGTCutSmart™ Buffer100502510065°C37°CNot SensitiveNot SensitiveBlockedpUC19 DNA
    HpyCH4V recombinant timesaver 5minTG/CACutSmart™ Buffer50502510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    I-CeuI recombinantTAACTATAACGGTCCTAAGGTAGCGAA(-9/-13)CutSmart™ Buffer10101010065°C37°CpBHS ScaI-linearized Control Plasmid
    I-SceI recombinantTAGGGATAACAGGGTAAT(-9/-13)CutSmart™ Buffer10502510065°C37°CpGPS2 NotI-linearized Control Plasmid
    KasI recombinant cpgG/GCGCCCutSmart™ Buffer501005010065°C37°CNot SensitiveNot SensitiveBlockedpBR322 DNA
    KpnI recombinantGGTAC/CNEBuffer 1.1100751050*No37°CNot SensitiveNot SensitiveNot SensitiveAdenovirus-2 DNA
    KpnI-HF® recombinant timesaver 5min engineeredGGTAC/CCutSmart™ Buffer1002510100No37°CNot SensitiveNot SensitiveNot SensitivepXba DNA
    KpnI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix iconGGTAC/C-----No37°C-Not SensitiveNot SensitiveNot Sensitive
    LpnPI recombinantCCDG(10/14)NEBuffer 4 + BSA1010105065°C37°CNot SensitiveNot SensitiveNot SensitivepBR322 (dcm+) DNA
    MboI recombinant timesaver 5min dam cpg/GATCCutSmart™ Buffer7510010010065°C37°CBlockedNot SensitiveImpaired by Overlapping λ DNA (dam-)
    MboII recombinant damGAAGA(8/7)CutSmart™ Buffer100*1005010065°C37°CBlocked by OverlappingNot SensitiveNot Sensitiveλ DNA (dam-)
    MfeI recombinantC/AATTGCutSmart™ Buffer755010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MfeI-HF® recombinant timesaver 5min engineeredC/AATTGCutSmart™ Buffer752510100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MfeI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix iconC/AATTG-----65°C37°C-Not SensitiveNot SensitiveNot Sensitive
    MluCI recombinant timesaver 5min/AATTCutSmart™ Buffer1001010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MluI recombinant timesaver 5min cpgA/CGCGTNEBuffer 3.110501002580°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    MlyI recombinant timesaver 5minGAGTC(5/5)CutSmart™ Buffer50501010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MmeI recombinant timesaver 5min cpgTCCRAC(20/18)CutSmart™ Buffer + SAM501005010065°C37°CNot SensitiveNot SensitiveBlocked by OverlappingΦX174 DNA
    MnlI recombinant timesaver 5minCCTC(7/6)CutSmart™ Buffer751005010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MscI recombinant dcmTGG/CCACutSmart™ Buffer2510010010080°C37°CNot SensitiveBlocked by OverlappingNot Sensitiveλ DNA
    MseI recombinant timesaver 5minT/TAACutSmart™ Buffer751007510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MslI recombinant timesaver 5minCAYNN/NNRTGCutSmart™ Buffer50501010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MspA1I recombinant timesaver 5min cpgCMG/CKGCutSmart™ Buffer10501010065°C37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNA
    MspI recombinant timesaver 5minC/CGGCutSmart™ Buffer7510050100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    MspJI recombinantCNNR(9/13)NEBuffer 4 + BSA1010105065°C37°CNot SensitiveNot SensitiveNot SensitivepBR322 (dcm+) DNA
    MwoI recombinant timesaver 5min cpgGCNNNNN/NNGCCutSmart™ Buffer10100100100No60°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    NaeI recombinant cpgGCC/GGCCutSmart™ Buffer252510100No37°CNot SensitiveNot SensitiveBlockedpXba DNA
    NarI recombinant cpgGG/CGCCCutSmart™ Buffer1001001010065°C37°CNot SensitiveNot SensitiveBlockedpXba DNA
    Nb.BbvCI recombinantCCTCAGCCutSmart™ Buffer2510010010080°C37°CNot SensitiveNot SensitiveNot Sensitivesupercoiled plasmid DNA
    Nb.BsmI recombinantGAATGCNEBuffer 3.110501001080°C65°CNot SensitiveNot SensitiveNot SensitivepBR322 DNA
    Nb.BsrDI recombinantGCAATGCutSmart™ Buffer2510010010080°C65°CNot SensitiveNot SensitiveNot SensitivepUC19 DNA
    Nb.BtsI recombinantGCAGTGCutSmart™ Buffer751007510080°C37°CNot SensitiveNot SensitiveNot SensitiveΦX174 DNA
    NciI recombinant timesaver 5min cpgCC/SGGCutSmart™ Buffer1002510100No37°CNot SensitiveNot SensitiveImpaired by Overlapping λ DNA
    NcoI recombinant timesaver 5minC/CATGGNEBuffer 3.110010010010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    NcoI-HF® recombinant timesaver 5min engineeredC/CATGGCutSmart™ Buffer501001010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    NcoI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix iconC/CATGG-----80°C37°C-Not SensitiveNot SensitiveNot Sensitive
    NdeI recombinant timesaver 5minCA/TATGCutSmart™ Buffer7510010010065°C37°CNot SensitiveNot SensitiveNot Sensitive
    NgoMIV recombinant timesaver 5min cpgG/CCGGCCutSmart™ Buffer1005010100No37°CNot SensitiveNot SensitiveBlockedAdenovirus-2 DNA
    NheI recombinant timesaver 5min cpgG/CTAGCNEBuffer 2.11001001010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA (HindIII digest)
    NheI-HF® recombinant timesaver 5min engineered cpgG/CTAGCCutSmart™ Buffer100251010080°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA (HindIII digest)
    NheI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix icon cpgG/CTAGC-----80°C37°C-Not SensitiveNot SensitiveBlocked by Some Combinations of Overlapping
    NlaIII recombinant timesaver 5minCATG/CutSmart™ Buffer10101010065°C37°CNot SensitiveNot SensitiveNot SensitiveΦX174 DNA
    NlaIV recombinant dcm cpgGGN/NCCCutSmart™ Buffer10101010065°C37°CNot SensitiveBlocked by OverlappingBlocked by Overlapping
    NmeAIII recombinantGCCGAG(21/19)CutSmart™ Buffer + SAM10101010065°C37°CNot SensitiveNot SensitiveNot SensitiveΦX174 RF I DNA
    NotI recombinant timesaver 5min cpgGC/GGCCGCNEBuffer 3.110501002565°C37°CNot SensitiveNot SensitiveBlockedpBC4 DNA
    NotI-HF® recombinant timesaver 5min engineered cpgGC/GGCCGCCutSmart™ Buffer251002510065°C37°CNot SensitiveNot SensitiveBlockedpBC4 DNA
    NotI-HF® RE-Mix®  recombinant timesaver 5min engineered re-mix icon cpgGC/GGCCGC-----65°C37°C-Not SensitiveNot SensitiveBlocked
    NruI recombinant timesaver 5min dam cpgTCG/CGANEBuffer 3.1101010010No37°CBlocked by OverlappingNot SensitiveBlockedλ DNA
    NsiI recombinant timesaver 5minATGCA/TNEBuffer 3.110751002565°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    NspI recombinant timesaver 5minRCATG/YCutSmart™ Buffer1001001010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    Nt.AlwI recombinant damGGATC(4/-5)CutSmart™ Buffer1010010010080°C37°CBlockedNot SensitiveNot SensitivepUC101 DNA (dam-/dcm-)
    Nt.BbvCI recombinant cpgCCTCAGC(-5/-7)CutSmart™ Buffer501001010080°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingsupercoiled plasmid DNA
    Nt.BsmAI recombinant cpgGTCTC(1/-5)CutSmart™ Buffer100501010065°C37°CNot SensitiveNot SensitiveBlockedsupercoiled plasmid DNA
    Nt.BspQI recombinantGCTCTTC(1/-7)NEBuffer 3.110251001080°C50°CNot SensitiveNot SensitiveNot SensitivepUC19 DNA
    Nt.BstNBI recombinantGAGTC(4/-5)NEBuffer 3.10101001080°C55°CNot SensitiveNot SensitiveNot SensitiveT7 DNA
    Nt.CviPII recombinant cpg(0/-1)CCDCutSmart™ Buffer101002510065°C37°CNot SensitiveNot SensitiveBlockedpUC19 DNA
    PacI recombinant timesaver 5minTTAAT/TAACutSmart™ Buffer100751010065°C37°CNot SensitiveNot SensitiveNot SensitivepNEB193 DNA
    PacI RE-Mix® recombinant timesaver 5min re-mix iconTTAAT/TAA-----65°C37°C-Not SensitiveNot SensitiveNot Sensitive
    PaeR7I recombinant timesaver 5min cpgC/TCGAGCutSmart™ Buffer2510010100No37°CNot SensitiveNot SensitiveBlockedλ DNA (HindIII digest)
    PciI recombinantA/CATGTNEBuffer 3.1507510050*80°C37°CNot SensitiveNot SensitiveNot SensitivepXba DNA
    PflFI timesaver 5minGACN/NNGTCCutSmart™ Buffer251002510065°C37°CNot SensitiveNot SensitiveNot SensitivepBC4 DNA
    PflMI recombinant timesaver 5min dcmCCANNNN/NTGGNEBuffer 3.101001005065°C37°CNot SensitiveBlocked by OverlappingNot Sensitiveλ DNA
    PhoI recombinant dcm cpgGG/CCCutSmart™ Buffer5050100100No75°CNot SensitiveImpaired by Some Combinations of OverlappingImpaired by Some Combinations of Overlappingλ DNA
    PI-PspI recombinantTGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17)NEBuffer PI-PspI + BSA10101010No65°CpAKR7 XmnI-linearized Control Plasmid
    PI-SceI recombinantATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19)NEBuffer PI-SceI + BSA1010101065°C37°CpBSvdeX XmnI-linearized Control Plasmid
    PleI recombinant cpgGAGTC(4/5)CutSmart™ Buffer25502510065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    PluTI recombinant cpgGGCGC/CCutSmart™ Buffer100251010065°C37°CNot SensitiveNot SensitiveBlockedpXba DNA
    PmeI recombinant timesaver 5min cpgGTTT/AAACCutSmart™ Buffer10501010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    PmlI timesaver 5min cpgCAC/GTGCutSmart™ Buffer100501010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA (HindIII digest)
    PpuMI recombinant timesaver 5min dcmRG/GWCCYCutSmart™ Buffer101010100No37°CNot SensitiveBlocked by OverlappingNot Sensitiveλ DNA (HindIII digest)
    PshAI recombinant timesaver 5min cpgGACNN/NNGTCCutSmart™ Buffer25501010065°C37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    PsiI recombinantTTA/TAACutSmart™ Buffer101001010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    PspGI recombinant dcm/CCWGGCutSmart™ Buffer2510050100No75°CNot SensitiveBlockedNot SensitiveT7 DNA
    PspOMI recombinant dcm cpgG/GGCCCCutSmart™ Buffer10101010065°C37°CNot SensitiveImpaired by Some Combinations of OverlappingBlocked by OverlappingpXba DNA
    PspXI recombinant cpgVC/TCGAGBCutSmart™ Buffer1010025100No37°CNot SensitiveNot SensitiveImpairedλ DNA (HindIII digest)
    PstI recombinant timesaver 5minCTGCA/GNEBuffer 3.1757510050*80°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    PstI-HF® recombinant timesaver 5min engineeredCTGCA/GCutSmart™ Buffer107550100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    PvuI recombinant timesaver 5min cpgCGAT/CGNEBuffer 3.1102510010No37°CNot SensitiveNot SensitiveBlockedpXba DNA
    PvuI-HF® recombinant timesaver 5min engineered cpgCGAT/CGCutSmart™ Buffer25100100100No37°CNot SensitiveNot SensitiveBlockedpXba DNA
    PvuII recombinant timesaver 5minCAG/CTGNEBuffer 3.150100100100*No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    PvuII-HF® recombinant timesaver 5min engineeredCAG/CTGCutSmart™ Buffer101010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    RsaI recombinant timesaver 5min cpgGT/ACCutSmart™ Buffer255010100No37°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    RsrII recombinant cpgCG/GWCCGCutSmart™ Buffer25751010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    SacI recombinant timesaver 5minGAGCT/CNEBuffer 1.1100501010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA (HindIII digest)
    SacI-HF® recombinant timesaver 5min engineeredGAGCT/CCutSmart™ Buffer10501010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA (HindIII digest)
    SacII recombinant timesaver 5min cpgCCGC/GGCutSmart™ Buffer101001010065°C37°CNot SensitiveNot SensitiveBlockedpXba DNA
    SalI recombinant timesaver 5min cpgG/TCGACNEBuffer 3.110101001065°C37°CNot SensitiveNot SensitiveBlockedλ DNA (HindIII digest)
    SalI-HF® recombinant timesaver 5min engineered cpgG/TCGACCutSmart™ Buffer1010010010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA (HindIII digest)
    SalI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix icon cpgG/TCGAC-----80°C37°C-Not SensitiveNot SensitiveBlocked
    SapI recombinant timesaver 5minGCTCTTC(1/4)CutSmart™ Buffer75501010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    Sau3AI recombinant cpg/GATCNEBuffer 1.1100501010065°C37°CNot SensitiveNot SensitiveBlocked by Overlappingλ DNA
    Sau96I recombinant dcm cpgG/GNCCCutSmart™ Buffer5010010010065°C37°CNot SensitiveBlocked by OverlappingBlocked by Overlappingλ DNA
    SbfI recombinant timesaver 5minCCTGCA/GGCutSmart™ Buffer50251010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SbfI-HF® recombinant timesaver 5min engineeredCCTGCA/GGCutSmart™ Buffer50251010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    ScaI recombinantAGT/ACTNEBuffer 3N/RN/R100N/R80°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    ScaI-HF® recombinant timesaver 5min engineeredAGT/ACTCutSmart™ Buffer1001001010080°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    ScaI-HF® RE-Mix® recombinant timesaver 5min engineered re-mix iconAGT/ACT-----65°C37°C-Not SensitiveNot SensitiveNot Sensitive
    ScrFI recombinant dcm cpgCC/NGGCutSmart™ Buffer10010010010065°C37°CNot SensitiveBlocked by OverlappingBlocked by Overlappingλ DNA
    SexAI recombinant dcmA/CCWGGTCutSmart™ Buffer100755010065°C37°CNot SensitiveBlockedNot SensitivepBC4 DNA (dcm-)
    SfaNI recombinant cpgGCATC(5/9)NEBuffer 3.110751002565°C37°CNot SensitiveNot SensitiveImpaired by Some Combinations of OverlappingΦX174 RF I DNA
    SfcI recombinantC/TRYAGCutSmart™ Buffer75502510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SfiI recombinant timesaver 5min dcm cpgGGCCNNNN/NGGCCCutSmart™ Buffer2510050100No50°CNot SensitiveImpaired by Overlapping Blocked by Some Combinations of OverlappingAdenovirus-2 DNA
    SfoI recombinant timesaver 5min cpg dcmGGC/GCCCutSmart™ Buffer50100100100No37°CNot SensitiveBlocked by Some Combinations of OverlappingBlockedλ DNA (HindIII digest)
    SgrAI recombinant cpgCR/CCGGYGCutSmart™ Buffer1001001010065°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    SmaI recombinant timesaver 5min cpgCCC/GGGCutSmart™ Buffer10101010065°C25°CNot SensitiveNot SensitiveBlockedλ DNA (HindIII digest)
    SmlI recombinantC/TYRAGCutSmart™ Buffer257525100No55°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SnaBI recombinant cpgTAC/GTACutSmart™ Buffer50*501010080°C37°CNot SensitiveNot SensitiveBlockedT7 DNA
    SpeI recombinant timesaver 5minA/CTAGTCutSmart™ Buffer751002510080°C37°CNot SensitiveNot SensitiveNot SensitiveAdenovirus-2 DNA
    SpeI RE-Mix® recombinant timesaver 5min re-mix iconA/CTAGT-----80°C37°C-Not SensitiveNot SensitiveNot Sensitive
    SpeI-HF® recombinant timesaver 5min engineeredA/CTAGTCutSmart™ Buffer25501010080°C37°CNot SensitiveNot SensitiveNot SensitivepXba DNA
    SphI-HF® recombinant timesaver 5min engineeredGCATG/CCutSmart™ Buffer50251010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SphI recombinantGCATG/CNEBuffer 2.11001005010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SspI recombinant timesaver 5minAAT/ATTNEBuffer SspI50100505065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SspI-HF® recombinant timesaver 5min engineeredAAT/ATTCutSmart™ Buffer2510010100No37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    StuI recombinant timesaver 5min dcmAGG/CCTCutSmart™ Buffer5010050100No37°CNot SensitiveBlocked by OverlappingNot Sensitiveλ DNA
    StyD4I recombinant timesaver 5min dcm cpg/CCNGGCutSmart™ Buffer1010010010065°C37°CNot SensitiveBlocked by OverlappingImpaired by Overlapping λ DNA
    StyI recombinant timesaver 5minC/CWWGGNEBuffer 3.110251001065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    StyI-HF® recombinant timesaver 5min engineeredC/CWWGGCutSmart™ Buffer251002510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    SwaI recombinant timesaver 5minATTT/AAATNEBuffer 3.110101001065°C25°CNot SensitiveNot SensitiveNot SensitiveM13mp19 RF I DNA
    TaqαI recombinant timesaver 5min damT/CGACutSmart™ Buffer507510010080°C65°CBlocked by OverlappingNot SensitiveNot Sensitiveλ DNA
    TfiI recombinant timesaver 5min cpgG/AWTCCutSmart™ Buffer50100100100No65°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    TliI recombinant cpgC/TCGAGNEBuffer 3 + BSA105010050No75°CNot SensitiveNot SensitiveImpairedλ DNA (HindIII digest)
    TseI timesaver 5min cpgG/CWGCCutSmart™ Buffer75100100100No65°CNot SensitiveNot SensitiveBlocked by Some Combinations of Overlappingλ DNA
    Tsp45I/GTSACCutSmart™ Buffer1005010100No65°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    Tsp509I/AATTNEBuffer 1N/RN/RN/RN/RNo65°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    TspMI timesaver 5min cpgC/CCGGGCutSmart™ Buffer50*75*50*100No75°CNot SensitiveNot SensitiveBlockedpUCAdeno plasmid DNA
    TspRI recombinant timesaver 5minNNCASTGNN/CutSmart™ Buffer255025100No65°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    Tth111I recombinant timesaver 5minGACN/NNGTCCutSmart™ Buffer2510025100No65°CNot SensitiveNot SensitiveNot SensitivepBC4 DNA
    XbaI recombinant timesaver 5min damT/CTAGACutSmart™ Buffer101007510065°C37°CBlocked by OverlappingNot SensitiveNot Sensitiveλ DNA (dam-/Hind III digest)
    XbaI RE-Mix® recombinant timesaver 5min re-mix icon damT/CTAGA-----65°C37°C-Blocked by OverlappingNot SensitiveNot Sensitive
    XcmI recombinantCCANNNNN/NNNNTGGNEBuffer 2.1101002510065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    XhoI recombinant timesaver 5min cpgC/TCGAGCutSmart™ Buffer7510010010065°C37°CNot SensitiveNot SensitiveImpairedλ DNA (HindIII digest)
    XhoI RE-Mix® recombinant timesaver 5min re-mix icon cpgC/TCGAG-----80°C37°C-Not SensitiveNot SensitiveImpaired
    XmaI recombinant timesaver 5min cpgC/CCGGGCutSmart™ Buffer25501010065°C37°CNot SensitiveNot SensitiveImpairedAdenovirus-2 DNA
    XmnI recombinant timesaver 5minGAANN/NNTTCCutSmart™ Buffer50751010065°C37°CNot SensitiveNot SensitiveNot Sensitiveλ DNA
    ZraI recombinant cpgGAC/GTCCutSmart™ Buffer100251010080°C37°CNot SensitiveNot SensitiveBlockedλ DNA
    Ligation and Recutting Notes
    a. Ligation is less than 10%.
    b. Ligation is 25% -75%.
    c. Recutting after ligation is less than 5%.
    d. Recutting after ligation is 50% -75%.
    e. Ligation and recutting after ligation is not applicable since the enzyme is either a nicking enzyme, is affected by methylation, or the recognition sequence contains variable sequences.

    Star Activity Notes
    1. Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of > 5%.
    2. Star activity may result from extended digestion.
    3. Star activity may result from a glycerol concentration of > 5%.
    *. May exhibit star activity in this buffer.
    Note: 
    1. The values listed in this table are approximate. They were obtained using each enzyme's specific unit assay substrate DNA.