McrA, McrBC and EcoKI Strain Phenotypes

  Mcr Mcr EcoK Strain Background
Strain A BC R M
1100 + + + +  
AB266 + + + +  
AB1157 + + + + Mrr+
AT2459 - + + +  
BNN93 - - - + C600
BNN102 - - - + C600
CH731* + ? ? ?  
CH1332* - ? ? ? CH731
CH1372* - ? ? ? CH731
C235 - + + +  
C600 *** + + + Y70; Mrr+
CES200 - + - + AB1157
CJ236** (#E4141S) - + + +  
CPB1293 - + + + W3110
CPB1321 - - - + W3110
CR63 - + + +  
CSR603 ? + + +  
c2813 - - - + WA802; Mrr+
DB1316* + - - + Mrr+
DH1 + + - + MM294; Mrr+
DH3 - + - + MM294; Mrr+
DH5 + + - + MM294; Mrr+
DH5aMCR* - Δ Δ Δ DH5; Mrr -
DH10B* - Δ Δ Δ MC1061; Mrr -
DL538* - - - + NM621
DS410* - + + +  
DM800 - + + +  
ED8641 - + - + WA803
ED8654 - + - + WA803
ED8739 - - - - WA803
ED8767 - - - - WA803
ER1370 + + + + NK7254 (JC1552)
ER1381 + + - + ER1370; Mrr+
ER1378 + - - + ER1370; Mrr+
ER1398 + - - + MM294; Mrr+
ER1414 + - - + W3110; Mrr+
ER1458 - - - + Y1084
ER1562 - - - + MM294; Mrr+
ER1563 - + - + MM294; Mrr+
ER1564 - + - + ER1370; Mrr+
ER1565 - - - + ER1370; Mrr+
ER1647* - Δ Δ Δ ER1370; Mrr -
ER1648 - Δ Δ Δ ER1370; Mrr -
ER1793*(#E4101S) - Δ Δ Δ ER1370; Mrr -
ER1821** (#E4102S) - Δ Δ Δ MM294; Mrr -
ER2267** (#E4103S) - Δ Δ Δ MM294; F+; Mrr -
ER2925(#E4109S) - - - + GM271; Mrr +
FS1585 - + + + C600
GM31* - +/- + + Mrr+/-
GM161 ? + + +  
GM271 - - - + Mrr+
GM272 ? + + +  
GM2159** + + + + Mrr+
GM2929** - - - + GM2163 recF
GW1002 - ? + +  
H680 - ? + +  
HB101 + Δ Δ Δ Mrr -
Hfr3000 YA149 - + + + HfrH
Hfr3000 - + + +  
Hfr Cavalli + - + +  
Hfr Hayes** + + + + Mrr+
HfrH thi - - + + +  
HfrP4X6 + + + +  
HfrR4** + + + + Mrr+
HL1228* - - - + NM621
JC7623** - + + + Mrr+
JH132 - Δ Δ Δ K802
JM83 (+) + + +  
JM101 + (+) + + JM83
JM107 - + - + MM294; Mrr+
JM107MA2 - - - + JM107
JM109 (-) (+) - + JM107
5K - + - + C600
K12 + + + +  
K802 see WA802  
K803 see WA803  
K10 + - + + Hfr Cavalli
KMBL1164 - ? + +  
KW251* - - - +  
LE392 - + - + ED8654
MB406* - + - +  
MC1040 (+) (+)  
MC1061 - - - + Mrr+
MM294 + + - + 1100; Mrr+
NM477 - - - - C600
NM494 - + - - pop101
NM514 - &/or + - + pop101
NM519 + + - +  
NM538 (-) (+) - + ED8654
NM539 - + - + NM538
NM554 - - - + MC1061
NM621 - - - + C600
NM646* - - - + WA802
NM664* - Δ Δ Δ WA802; Mrr -
NM670* - + + + WA802
NM673* - - - + WA802
NM674* - Δ Δ Δ WA802; Mrr -
NW1* - - - + AT2459
NW2* - Δ Δ Δ AT2459; Mrr -
P678** + ? ? +  
PA309 + + + +  
PC0543** + - + + Mrr+
PC0950 + - + + Mrr+
Q358 - + - +  
Q359 - + - +  
RL88 - - + +  
RR1 + Δ Δ Δ HB101; Mrr -
SK5022 - + + +  
TC410* - Δ Δ Δ DS410; Mrr -
TC600* - - - + C600B
W6 + + + +  
W677** + ? + +  
W3110 + + + + Mrr+
W4797 + + + +  
WA704 - - + + C600 X HfrCavalli
WA802 - - - + WA704; Mrr+
WA803 - - - - WA704; Mrr+
WW3352 - + + +  
X149 - + + +  
Y10 + + + +  
Y53 + + + + Y10
Y70 + + + + Y53
Y1084 - + + + BNN96; SG1041
Y1088 - + - + LE392; ED8654
Y1090 (-) (+) + + Y1084
Non-K12 strains
E. coli B + + + +  
E. coli B/r ? + + +  
BL21** + + - - restricts l.Sss I
E. coli C - (-) - -  

Mcr phenotypes reported in [Raleigh, E.A. et al. (1988) Nucl. Acids Res., 16, 1563-1575 PMID: 2831502, References 7 in Restriction of Foreign DNA by E. coli K-12] except those denoted with asterisks. Mrr phenotypes determined at NEB except DH10B (11). EcoKI phenotypes sometimes inferred from genotype.

Table Notes:

  • ( ) phenotype inferred from that of ancestral or descendant strains.
  • ? phenotype not tested.
  • Δ mutation present is known to be a large deletion.
  • ‡ These strains were formerly thought to carry the mcrB allele present in E.coli B. They are now known to carry an extensive deletion of mrr -hsd -mcrBC (ref. 6; M. Noyer-Weidner, personal communication; J. Kelleher, personal communication).
  • * Phenotypes reported in one of references 8-12.
  • ** Phenotypes determined at New England Biolabs, Inc.
  • Orange catalog numbers in paretheses indicate strains that are available upon request from New England Biolabs at no charge with an order or for the cost of shipping if ordered separately.
  • *** C600 lines obtained from different sources give different results. The original strain, and that obtained from the E. coli Genetic Stock Center (Yale University) are McrA+; derivatives traceable to the Brenner laboratory are McrA- (21).