The gene encoding this enzyme has been cloned at New England Biolabs, Inc. This enzyme is purified from a recombinant source. This enzyme has been engineered. This enzyme will digest unit substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions. Indicates which reaction NEBuffer is supplied with the enzyme for optimal activity. Enzymes with buffer requirements not met by one of the four standard NEBuffers (1.1, 2.1, 3.1 or CutSmart) are supplied with their own unique NEBuffer (NEBuffer U). NEBuffers are color-coded (NEBuffer 1.1-yellow, NEBuffer 2.1-blue, NEBuffer 3.1-red, CutSmart-green) and supplied as 10X stocks with each enzyme. For more information, consult the NEBuffer Performance Chart.