• My NEB
  • Print
  • PDF
  • Glycobiology Unit Conversion Chart

    Reagent companies differ in how a unit of enzyme is defined. This chart can be used to help determine how a unit of enzyme from one company compares to a unit of enzyme from NEB. All enzymes were assayed using NEB's assay protocols as a means of normalization (NEB Assay).

    Unit Conversion: 1 Company unit = NEB Assay Concentration/Selling Concentration.

    Enzyme Company Selling Conc. (U/ml) Units /Vial µl/Vial NEB Assay (U/ml) NEB Assay Units /Vial Unit Conversion µl Conversion (1 NEB µl = x Company µls)
    PNGase F NEB (NEB #P0704) 500,000 15,000 30 500,000 15,000 1 1
    Prozyme 2 0.1 50 90,000 4,500 45,000 5.5
    QA Bio 5 0.1 22.5 200,000 4,500 40,000 2.5
    Sigma 500 50 50 90,000 4,500 180 5.5
    α1-6 Mannosidase NEB (NEB #P0727) 40,000 800 20 40,000 800 1 1
    Prozyme (GKX-80070) 0.2 .008 40 1,000 40 5,000 40
    Prozyme (GKX 5010, α1-2,3,6 Man) 150 10 67 4,000 268 27 10
    QA Bio (E-AMO1, α1-2,3,6 Man) 10 0.6 60 2,000 120 200 20
    α1-2,3 Mannosidase NEB (NEB #P0729) 32,000 640 20 32,000 640 1 1
    Prozyme (GKX 5010, α1-2,3,6 Man) 150 10 67 4,000 268 27 8
    QA Bio (E-AMO1, α1-2,3,6 Man) 10 0.6 60 700 42 70 45
    β1-4 Galactosidase NEB (NEB #P0730) 8,000 400 50 8,000 400 1 1
    Prozyme (GKX-5014) 2 0.2 100 4,000 400 2,000 2
    Prozyme (GKX 5013, β1-3,4 Gal) 5 0.5 100 1,000 100 200 8
    QA Bio (E-BG07) 3 0.18 60 40,000 2,400 13,300 0.2
    QA Bio (E-BG02, β1-3,4,6 Gal) 2.5 0.5 200 4,000 800 1,600 2
    β1-3 Galactosidase NEB (NEB #P0726) 10,000 500 50 10,000 500 1 1
    Prozyme (GKX 5013, β1-3,4 Gal) 5 0.5 12.5 8,000 100 1,600 1.25
    QA Bio (E-BG02, β1-3,4,6 Gal) 2.5 0.5 200 2,000 400 800 5
    β-N-Acetyl glucosaminidase NEB (NEB #P0732) 4,000 100 25 4,000 100 1 1
    QA Bio (E-GL01) 40 2.4 60 2,000 120 50 2
    α-N-Acetyl galactosaminidase NEB (NEB #P0734) 20,000 3,000 150 20,000 3,000 1 1
    Prozyme (GKX-5001) 5 0.1 20 600 12 120 33
    α1-3,6 Galactosidase NEB (NEB #P0731) 4,000 100 25 4,000 100 1 1
    Prozyme (GKX-5007, α1-3,4,6 Gal) 100 5 50 80,000 4,000 800 0.05
    Neuraminidase NEB (NEB #P0720) 50,000 2,000 40 50,000 2,000 1 1
    Prozyme (GKX-5021) 5 1 200 15,000 3,000 3,000 3.3
    α2-3 Neuraminidase NEB (NEB #P0728) 5,000 2,500 50 50,000 2,500 1 1
    Prozyme (GK80020) 20 1 50 80,000 4,000 4,000 0.625
    β-N-Acetyl hexosaminidasef NEB (NEB #P0721) 5,000 500 100 5,000 500 1 1
    Prozyme (GKX-5003) 50 5 100 500 50 10 10