• My NEB
  • Print
  • PDF
  • Activity of DNA Modifying Enzymes in CutSmart Buffer

    Enzyme Activity in CutSmart Buffer Required Supplements
    Alkaline Phosphatase (CIP) +++  
    Antarctic Phosphatase +++  Requires Zn2+
    Bst DNA Polymerase +++  
    CpG Methyltransferase (M. SssI) +++  
    DNA Polymerase I +++  
    DNA Polymerase I, Large (Klenow) Fragment
    +++  
    DNA Polymerase Klenow Exo- +++  
    DNase I (RNase-free) +++ Requires Ca2+
    E. coli DNA Ligase +++ Requires NAD
    Endonuclease III (Nth), recombinant +++  
    Endonuclease VIII +++  
    Exonuclease III +++  
    GpC Methyltransferase (M. CviPI) + Requires DTT
    McrBC +++  
    Micrococcal Nuclease +++  
    Nuclease BAL-31 +++  
    phi29 DNA Polymerase +++  
    RecJf +++  
    T3 DNA Ligase +++ Requires ATP + PEG
    T4 DNA Ligase +++ Requires ATP
    T4 DNA Polymerase +++  
    T4 Phage β-glucosyltransferase (T4-BGT) +++  
    T4 Polynucleotide Kinase +++ Requires ATP
    T4 PNK (3 ' phosphatase minus) +++ Requires ATP
    T7 DNA Ligase +++ Requires ATP + PEG
    T7 Exonuclease +++  
    USER Enzyme, recombinant
    +++  

    +++ full functional activity
    ++ 50-100% functional activity
    + 0-50% functional activity