Transfection Reagents Selection Chart

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  • NEB offers a broad selection of high efficiency transfection reagents suitable for transfecting DNA, siRNA and protein. For the transfection of DNA and siRNA, choose from a selection of general purpose reagents or reagents optimized for specific cell lines. TransPass™ P delivers intact functional proteins, peptides or antibodies into mammalian cells with exceptional efficiency. The table below lists the transfection reagents available from NEB.

    Transfection Reagent NEB # Number of Transfections* Validated Cell Types
    For DNA
    TransPass™ D1 M2553S 65-200 A549, COS-7, SL2 (Drosophila), HEK293, HeLa, HO23, HMVEC, HUVEC, Lung microvascular endothelial cells, MCF-7, rat, mouse and human primary hepatocytes, NIH 3T3, RBE4, SL2, U20S, ARPE, RBE4, human and bovine aortic endothelial cells
    M2553L 325-1,000
    TransPass™ D2 M2554S 50-100 A549, BAEC, CHO-K1, COS-7, HCT-116, HepG2, HEK293, HUVEC, HeLa, HL60, Jurkat, MDA, NIH 3T3, SL2, U2OS, ARPE, 293T, MCF-7
    M2554L 250-500
    TransPass™ COS/293 M2557S 100-200 COS-7, HEK293
    TransPass™ HUVEC M2558S 150-250 HUVEC cells, Dermal microvascular endothelial cells, Lung microvascular endothelial cells, Human aortic endothelial cells, Bovine aortic endothelial cells, Rat endothelial cells
    TransPass™ V M2561S 75-125 Human: HepG2, Huh-7, MCF-7, Jurkat, IMR-90, A549, T47D, ARPE, MDCK, MEF, mES (mouse embryonic stem cells), SL2 (Drosophila)
    For siRNA
    TransPass™ R2 M2552S 200 Primary Cells: β-lymphoma cells, HMVEC, HUVEC, Human lens epithelial cells, Rat lens epithelial cells, Vascular smooth muscle cells.
    Cell Lines: A549, COS-7, HCT116, HEK293, HeLa, HepG2, NIH 3T3, OVCAR-3, Huh-7, MCF-7, Human cytrophoblasts, Human and rat cardiac stem cells, Rat primary cardiac myoblasts, PTEC, C2C12, RAW 264.7, MDM
    For Protein
    TransPass™ P M2563S 125-250**  

    * Number of reansfections is based on a 6-well plate format.
    ** Number of transfections is determined for 1µg protein.

    As a service to our customers, NEB offers a select group of transfection reagents that are manufactured for NEB. Quality controls are performed by both the suppliers and New England Biolabs. Specific information can be found on the product datacard.

    TransPass™ is a trademark of New England Biolabs, Inc.