SNAP-tag®/CLIP-tag® Cloning Vector and Plasmid Selection Chart

Vectors are available for SNAP-, CLIP-, ACP- and MCP-tag fusion protein expression and labeling in mammalian and bacterial systems. The mammalian SNAPf and CLIPf vectors express faster-reacting variants of the SNAP- and CLIP-tags than previously available vectors. Improved polylinker sequences both upstream and downstream from the tag allow expression of the tag on either end of the protein of interest, under control of the CMV promoter. SNAPf-tag and CLIPf-tag expression vectors contain a neomycin resistance (NeoR) gene for selection of stable transfectants, together with an IRES element for efficient expression of both the fusion protein and NeoR. The ACP-tag and MCP-tag expression vectors are under control of the CMV promoter. The ACP-tag expression vector is suitable for transient or stable expression, but the MCP-tag expression vector is suitable for transient expression only. Codon usage has been optimized for mammalian expression. Control plasmids encoding fusion proteins that are localized to the nucleus (H2B), mitochondria (Cox8A) and cell surface (ADRβ2, NK1R, GPI) are also available (see table below). The pSNAP-tag®(T7)-2 Vector (NEB #N9322) IS an Escherichia coli expression plasmid encoding the SNAP-tag protein. The codon usage of the SNAP26b gene is optimized for expression in E. coli. Expression is under control of the IPTG inducible T7 promoter.

Product NEB# Features Size
pSNAPf Vector N9183 stable and transient mammalian expression 20 μg
pSNAP-tag® (T7)-2 Vector N9181 bacterial expression under T7 control 20 μg
pSNAPf-Cox8A Control Plasmid N9185 mitochondrial localization 20 μg
pSNAPf-ADRβ2 Control Plasmid N9184 cell surface localization 20 μg
pSNAPf-H2B Control Plasmid N9186 nuclear localization 20 μg
pCLIPf Vector N9215 stable and transient mammalian expression 20 μg
pCLIPf-Cox8A Control Plasmid N9217 mitochondrial localization 20 μg
pCLIPf-NK1R Control Plasmid N9216 cell surface localization 20 μg
pCLIPf-H2B Control Plasmid N9218 nuclear localization 20 μg
pACP-tag(m)-2 Vector N9322 stable and transient mammalian expression 20 μg
pACP-ADRβ2 Control Plasmid N9321 cell surface localization 20 μg
pMCP-GPI Control Plasmid N9320 cell surface localization 20 μg
pMCP-tag(m) Vector N9317 transient mammalian expression 20 μg

SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag® is a trademark of New England Biolabs, Inc.