SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart

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  • NEB offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker, while CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. These substrates will label their respective tags without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell® and CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface® and CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.

    ACP/MCP-tag substrates are conjugated to the phosphopantetheinyl moeity of Coenzyme A (CoA) and require a separate enzyme (ACP or SFP Synthase) for labeling. The labeling reaction is specific for fusion proteins expressed on the cell surface. The same substrates are used to label both the ACP- and the MCP-tags; specificity is determined by the synthase used for labeling.

    Self-Labeling Tags

    SNAP-tag Cell-Permeable

    SubstratesNEB #Excitation*Size
    Emission* ✝
    SNAP-Cell® 360#S9101357437
    50nmol
    SNAP-Cell® 505-Star#S9103504532
    50nmol
    SNAP-Cell® Fluorescein#S9107500532
    50nmol
    SNAP-Cell® Oregon Green®#S9104490514
    50nmol
    SNAP-Cell® TMR-Star#S9105554580
    30nmol
    SNAP-Cell® 430#S9109421444485
    50nmol

    Non-cell-permeable

    SubstratesNEB #Excitation*Size
    Emission* ✝
    SNAP-Surface® 425#S9126438489
    50nmol
    SNAP-Surface® 488#S9124506526
    50nmol
    SNAP-Surface® 532#S9131536554
    50nmol
    SNAP-Surface® 549#S9112560575
    50nmol
    SNAP-Surface® 594#S9134606626
    50nmol
    SNAP-Surface® 647#S9137660673
    50nmol
    SNAP-Surface® 649#S91590
    50nmol
    SNAP-Surface® 682#S9139693708
    50nmol
    SNAP-Surface® 782#S9142784789
    50nmol
    SNAP-Surface® Alexa Fluor® 488#S9129496520
    50nmol
    SNAP-Surface® Alexa Fluor® 546#S9132558574
    50nmol
    SNAP-Surface® Alexa Fluor® 647#S9136652670
    50nmol

    CLIP-tag Cell-Permeable

    SubstratesNEB #Excitation*Size
    Emission* ✝
    CLIP-Cell™ 360#S9215357437
    50nmol
    CLIP-Cell™ 430#S9216421444484
    50nmol
    CLIP-Cell™ 505#S9217504532
    50nmol
    CLIP-Cell™ Fluorescein#S9218500532
    50nmol
    CLIP-Cell™ TMR-Star#S9219554580
    30nmol

    Non-cell-permeable

    SubstratesNEB #Excitation*Size
    Emission* ✝
    CLIP-Surface™ 488#S9232506526
    50nmol
    CLIP-Surface™ 547#S9233554568
    50nmol
    CLIP-Surface™ 647#S9234660673
    50nmol

    Enzymatic Tag

    ACP/MCP tag Non-cell-permeable

    SubstratesNEB #Excitation*Size
    Emission* ✝
    CoA 488#S9348506526
    50nmol
    CoA 547#S9349554568
    50nmol
    CoA 647#S9350660673
    50nmol

    * Excitation and emission values determined experimentally for labeled protein tag.
    † Colors are based on the electromagnetic spectrum. Actual color visualization may vary.

    SNAP-tag®, SNAP-Cell®, SNAP-Surface® and NEB® are registered tradmarkes of New England Biolabs, Inc.
    CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.